[PDF] Top 20 A conditional lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis
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A conditional lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis
... A conditional-lethal recombinant virus was constructed in which the expression of the vaccinia virus I7L gene is under the control of the tetracycline operator/repressor ... See full document
6
Vaccinia virus A17L gene product is essential for an early step in virion morphogenesis.
... D13L gene product (p65 membrane protein), which is the target of rifampin (18, 37), is required at an early stage of virion morphogenesis prior to formation of immature viral envelopes ... See full document
9
Cell Biological and Functional Characterization of the Vaccinia Virus F10 Kinase: Implications for the Mechanism of Virion Morphogenesis
... first lethal phenotype is ...F10, morphogenesis arrests prior to the ap- pearance of any discernible viral ...of virion assembly or infectivity has remained ...wt virus or ts F10 mutants and ... See full document
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Vaccinia Virus Nonstructural Protein Encoded by the A11R Gene Is Required for Formation of the Virion Membrane
... dead-end product and thereby arresting morpho- ...arrest morphogenesis by some other mech- anism, resulting in the inappropriate insertion of viral mem- brane proteins into the ER, causing as a secondary ... See full document
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Vaccinia virus morphogenesis is blocked by temperature-sensitive mutations in the F10 gene, which encodes protein kinase 2.
... Several vaccinia virus-encoded proteins are phosphorylated in ...F17 gene [16]) that is encapsidated within the infectious ...the virion and is phosphory- lated in vivo at two different serine ... See full document
13
Vaccinia Virus A21 Virion Membrane Protein Is Required for Cell Entry and Fusion
... a conditional lethal VACV mutant vE10i (42) in the absence of inducer so that expression of the E10L gene product did not ...parental virus vT7LacOI were transfected in parallel; ... See full document
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Vaccinia Virus A6L Encodes a Virion Core Protein Required for Formation of Mature Virion
... total virion, along with virion membrane proteins L1 and A27 (19, ...the virion core with deoxycholate and DTT also failed to re- lease any A6, indicating that A6 is tightly packaged in the ... See full document
11
Vaccinia Virus F9 Virion Membrane Protein Is Required for Entry but Not Virus Assembly, in Contrast to the Related L1 Protein
... 41); virion maturation, e.g., A9 and L1 (24, 43); virus attachment to glycosaminoglycans, ...and virus entry, ...in virus entry have been considered previously because they are targets of ... See full document
10
A Glutaredoxin, Encoded by the G4L Gene of Vaccinia Virus, Is Essential for Virion Morphogenesis
... Vaccinia virus encodes two glutaredoxins, O2L and G4L, both of which exhibit thioltransferase and dehy- droascorbate reductase activities in ...for virus replication, we now show that G4L is ... See full document
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The Conserved Poxvirus L3 Virion Protein Is Required for Transcription of Vaccinia Virus Early Genes
... conditionally lethal mutant VACVs that produce virions with normal appearance and DNA content have been described elsewhere (8, 14, 18, 27, 29, 33, ...in virus entry (26, 27) or early transcription ... See full document
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Clustered Charge-to-Alanine Mutagenesis of the Vaccinia Virus H5 Gene: Isolation of a Dominant, Temperature-Sensitive Mutant with a Profound Defect in Morphogenesis
... of morphogenesis at the nonpermissive ...viral morphogenesis, including the ap- pearance of electron-dense virosomes, the emergence of mem- brane crescents near the virosome, and the formation of immature, ... See full document
13
Analysis of a Vaccinia Virus Mutant Expressing a Nonpalmitylated Form of p37, a Mediator of Virion Envelopment
... the culture medium by repeatedly pipetting the medium over the cell monolayer. The cell suspensions were subjected to centrifugation at 12,000 3 g and 4°C for 30 min to pellet cells and virus particles. The ... See full document
13
Analysis of the Role of Vaccinia Virus H7 in Virion Membrane Biogenesis with an H7-Deletion Mutant
... accinia virus (VACV) is a member of the Poxviridae family of large cytoplasmic DNA viruses ...place. Virion assembly goes through a series of inter- mediate stages discernible by electron microscopy (EM) ... See full document
7
Structure/Function Analysis of the Vaccinia Virus F18 Phosphoprotein, an Abundant Core Component Required for Virion Maturation and Infectivity
... these virion preparations for transcription in situ ...the virion core or severely alter the accessibility of the genome to the encapsidated transcriptional ... See full document
15
The A2L intermediate gene product is required for in vitro transcription from a vaccinia virus late promoter.
... the gene for VLTF-X has not yet been ...was required for in vitro transcription when traditional preparations of VLTF-X (obtained from late ex- tracts) were employed ... See full document
5
Replication of a Nuclear Polyhedrosis Virus in a Continuous Cell Culture of Spodoptera frugiperda: Microscopy Study of the Sequence of Events of the Virus Infection
... Basically, there are nine significant morphogenic events: entry, uncoating, virion morphogenesis, envelope morphogenesis, release, polyhedron formation, occlusion of enveloped virus, pol[r] ... See full document
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Vaccinia Virus Mutations in the L4R Gene Encoding a Virion Structural Protein Produce Abnormal Mature Particles Lacking a Nucleocapsid
... the vaccinia virion core. Recently, we showed that packaging of virus transcription enzymes is necessary for the formation of the tubular structure, suggesting that the structure is equivalent to a ... See full document
13
The Vaccinia Virus B5 Protein Requires A34 for Efficient Intracellular Trafficking from the Endoplasmic Reticulum to the Site of Wrapping and Incorporation into Progeny Virions
... The lumenal domain of A34 interacts with B5-GFP. The most straightforward explanation for our results is that A34 and B5 interact through their lumenal domains and that this interaction is required for the proper ... See full document
9
The Vaccinia Virus A14.5L Gene Encodes a Hydrophobic 53-Amino-Acid Virion Membrane Protein That Enhances Virulence in Mice and Is Conserved among Vertebrate Poxviruses
... a gene with a T7 promoter in vaccinia ...cinia virus was constructed in which the HA tag was inserted between the C-terminal and stop codons of the endogenous ...the gene remained under its ... See full document
8
Use of a cell-free system to identify the vaccinia virus L1R gene product as the major late myristylated virion protein M25.
... The identity of the 25-kDa polypeptide labeled in vivo by [3H]myristic acid M25 as the gene product of ORF LiR was further established by immunoprecipitation of either extracts of purifi[r] ... See full document
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