[PDF] Top 20 Conditional Mutations in the Mitotic Chromosome Binding Function of the Bovine Papillomavirus Type 1 E2 Protein
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Conditional Mutations in the Mitotic Chromosome Binding Function of the Bovine Papillomavirus Type 1 E2 Protein
... well-spread mitotic chromosomes and demon- strated that the wild-type E2-TA was localized on individual chromosomes as speckles, whereas E2-TR was excluded from ...the E2 protein ... See full document
10
Structural, functional, and protein binding analyses of bovine papillomavirus type 1 exonic splicing enhancers.
... Point mutations and deletions which would be expected to eliminate splicing enhancer function were made in SE1 and SE2 ...exon 1 and exon 2 ... See full document
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Bovine papillomavirus type 1 DNA replication: the transcriptional activator E2 acts in vitro as a specificity factor.
... of bovine papillomavirus type 1 (BPV1) DNA ...initiator protein E1, together with extract from a particular cell source, allowed the synthesis of complete DNA circles through successive ... See full document
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Isolation of an amino-terminal region of bovine papillomavirus type 1 E1 protein that retains origin binding and E2 interaction capacity.
... 1700T protein, we constructed a series of inter- nal, in-frame deletion and insertion mutations by the previ- ously described procedure of Ludes-Meyers and Wilson ...Table 1. Expression of each ... See full document
5
Transcriptional activation function is not required for stimulation of DNA replication by bovine papillomavirus type 1 E2.
... replication, E2 possesses a TAD and a DNA- binding domain which serve to activate transcription from viral promoters and to stimulate replication from the PV ...replication, E2 has an addi- tional ... See full document
6
Characterization of the Functional Activities of the Bovine Papillomavirus Type 1 E2 Protein Single-Chain Heterodimers
... Three papillomavirus proteins encoded by the bovine papillomavirus type 1 E2 open reading frame have a common C-terminal DNA binding and dimerization domain and ... See full document
8
Amino acids critical for the functions of the bovine papillomavirus type 1 E2 transactivator.
... mutated E2 proteins were found to be completely defective for transcriptional activation, and their replication functions were severely ...the E2 protein, and it is possible that these residues are ... See full document
7
Long-Term Episomal Maintenance of Bovine Papillomavirus Type 1 Plasmids Is Determined by Attachment to Host Chromosomes, Which Is Mediated by the Viral E2 Protein and Its Binding Sites
... multimerized E2 protein binding sites determine the chromatin attachment of the plasmids in the ...(Fig. 1) in the same plasmid context, pNeo59, was transfected into the BPV1 E1- and ... See full document
9
Competitive binding of viral E2 protein and mammalian core-binding factor to transcriptional control sequences of human papillomavirus type 8 and bovine papillomavirus type 1.
... The mutations in P2C outside the CBF consensus site had also significantly decreased the basal promoter activity ...PEBP2 protein than P2TATA, pointing to another tran- scription-activating factor affected ... See full document
6
Transcriptional and replicational activation functions in the bovine papillomavirus type 1 E2 protein are encoded by different structural determinants.
... that mutations K111A, K112A, and E176A seem to abolish the DNA binding abilities of these proteins and therefore the functioning of the E2 protein in transcription and replication, or to ... See full document
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The Human Papillomavirus Type 8 E2 Tethering Protein Targets the Ribosomal DNA Loci of Host Mitotic Chromosomes
... HPV-8 E2 binds to the pericentromeric region of a subset of mitotic ...the mitotic binding site of HPV-8 E2, E2-expressing cell lines were derived from human cervical ... See full document
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Effect of Bovine Papillomavirus E2 Protein-Specific Monoclonal Antibodies on Papillomavirus DNA Replication
... The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and ...BPV-1 E2 protein and used them ... See full document
8
A Novel Silencer Element in the Bovine Papillomavirus Type 4 Promoter Represses the Transcriptional Response to Papillomavirus E2 Protein
... sequence-specific binding in both PalK and PalF ...enhanced binding in PalK cells to ...of binding, four copies of PRE-2mt1 were inserted upstream of the SV40 promoter in the pGL3PRO reporter vector, ... See full document
10
Phosphorylation Regulates Binding of the Human Papillomavirus Type 8 E2 Protein to Host Chromosomes
... the E2 protein in S phase and the daughter molecules remain bound throughout ...the chromosome binding regions of EBNA1 and LANA further un- derscores the possibility of a common mechanism ... See full document
12
Amino-terminal domains of the bovine papillomavirus type 1 E1 and E2 proteins participate in complex formation.
... fusion protein containing aa 53 to 161 of BPV-1 E2 had an intrinsic transcriptional activation capacity in yeast cells, suggesting the possible presence of a discrete tran- scription activation ... See full document
9
Dimerization of the Papillomavirus E2 Protein Is Required for Efficient Mitotic Chromosome Association and Brd4 Binding
... The E2 proteins of several papillomaviruses link the viral genome to mitotic chromosomes to ensure retention and the efficient partitioning of genomes into daughter cells following cell ...division. ... See full document
8
Bovine Papillomavirus Type 1 Genomes and the E2 Transactivator Protein Are Closely Associated with Mitotic Chromatin
... Fluorescent in situ hybridization (FISH). Cells were grown on glass slides and treated with colchicine as described above. Cells were fixed for 20 min in meth- anol-acetic acid (3:1). Where indicated, cells were ... See full document
10
Interaction of Bovine Papillomavirus E2 Protein with Brd4 Stabilizes Its Association with Chromatin
... competent E2 proteins colocalize with Brd4 on mitotic ...mutated E2 proteins that are defective in specific E2 functions was previously de- scribed (3, ...E1 protein, interaction with ... See full document
13
E1 Protein of Bovine Papillomavirus Type 1 Interferes with E2 Protein-Mediated Tethering of the Viral DNA to Mitotic Chromosomes
... in E2 at residues 298 and 301 by themselves lead to an increase in E2 trans-acting activity in transient assays (12) and increased E2 protein accumulation and a longer half-life for mutant ... See full document
12
The Mitotic Chromosome Binding Activity of the Papillomavirus E2 Protein Correlates with Interaction with the Cellular Chromosomal Protein, Brd4
... BPV1 E2 protein mediates several key functions in the life cycle of the ...to mitotic chromosomes to enable stable ge- nome ...segregation function requires the in- teraction of the ... See full document
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