[PDF] Top 20 DEPS 1 promotes P granule assembly and RNA interference in C elegans germ cells
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DEPS 1 promotes P granule assembly and RNA interference in C elegans germ cells
... in deps-1 germ lines (9/32) are also upregulated in mixed-stage mRNA preparations made from rde- 3(ne298) worms (Table 2), and many of these are strongly upregulated in rde-3 worms (5/9 increased ... See full document
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GLH 1, the C elegans P granule protein, is controlled by the JNK KGB 1 and by the COP9 subunit CSN 5
... In C. elegans, regulation of protein degradation has been elegantly examined in relation to embryonic patterning and the transition from oocyte to embryo (DeRenzo et ...The C. elegans germline ... See full document
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Insulin signaling promotes germline proliferation in C elegans
... glp- 1(ar202) Pro phenotype and reduction of germ cell numbers (see Tables S3, S6 in the supplementary ...on germ cell number and mitotic index were daf-18 and daf-16 dependent ...proliferative ... See full document
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A conserved RNA protein complex component involved in physiological germline apoptosis regulation in C elegans
... CAR- 1, an apparent RNA-binding protein, results in two phenotypes that are consistent with cytoskeletal or membrane abnormalities (ACS production and a cytokinesis ...spindle assembly (Blower et ... See full document
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A germ cell determinant reveals parallel pathways for germ line development in Caenorhabditis elegans
... food. Germ cells enter meiosis midway through the third larval stage (L3) and, in the hermaphrodite, the first germ cells differentiate into sperm before switching to oogenesis in L4 (Kimble ... See full document
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The P granule component PGL 1 promotes the localization and silencing activity of the PUF protein FBF 2 in germline stem cells
... bound RNA was eluted with 100 mM glycine pH ...Before RNA isolation, an aliquot was removed from each eluate to quantify the target protein by quantitative western ...Total RNA was isolated from ... See full document
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Less is more: specification of the germline by transcriptional repression
... the germ granules are perinuclear and are often located close to nuclear pores, raising the possibility that many, if not all, germline mRNAs interact with germ granules upon exit from the nucleus (Eddy, ... See full document
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An antiviral role for the RNA interference machinery in Caenorhabditis elegans
... protects cells against viral infection, mutants that have an enhanced RNAi response (23–25) would be expected to be more resistant to virus than wild ...tant cells with VSV. eri-1 encodes an ... See full document
6
Trans-generational epigenetic regulation of C. elegans primordial germ cells
... the P blas- tomeres and its appearance in Z2/Z3 soon after their birth [13] (Figures 1B, Figure ...to RNA interference (RNAi) directed towards ama-1 (encoding the largest subunit of Pol II), ... See full document
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Perinuclear P granules are the principal sites of mRNA export in adult C elegans germ cells
... through P granules As viewed by electron microscopy, the cytoplasmic fibrils of NPCs are approximately 50 nm in length (Stoffler et ...the P granule begins approximately 70 nm from the nuclear ...the ... See full document
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The C elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules
... Several studies have implicated nucleoporins in activities beyond nuclear trafficking, including roles in chromosome segregation, spindle assembly and the regulation of transcription (reviewed by Lim et al., 2008; ... See full document
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Cell Biology of the Caenorhabditis elegans Nucleus
... embryos, P granules form large perinuclear structures, which, at least in gonads, are adjacent to clusters of NPCs (Figure 10) (Pitt et ...perinuclear P granules, electron-dense material accumulates in the ... See full document
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Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus
... 18 cells, the five fields selected from the FIV Gag MACA contained 15 cells, and the five fields selected from the FIV Gag G2A group contained 21 ...five 1- m slices centered on the focal point for the ... See full document
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Characterization of Virus-Encoded RNA Interference Suppressors in Caenorhabditis elegans
... in C. elegans exhibits several distinct ...on C. elegans RDVI suggest that the worm RDVI is initiated upon the processing of viral dsRNAs into primary siRNAs by the single worm Dicer, ... See full document
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FGF9 promotes survival of germ cells in the fetal testis
... somatic cells of the XY ...somatic cells is sufficient to induce the differentiation of Sertoli cells (Koopman et ...Sertoli cells are a male-specific somatic cell type that drive testis ... See full document
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Multiple maternal proteins coordinate to restrict the translation of C elegans nanos 2 to primordial germ cells
... all cells and stronger expression in a few cells at the posterior of the embryo – a pattern identical to the spn-4(RNAi) ...all cells of the embryo when both direct repeats were simultaneously ... See full document
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A V0 ATPase dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane
... To identify new common regulators of polarity and brush border maintenance in vivo, we performed a two-step RNAi screen targeting 408 genes essential for survival, conserved in humans and implicated in membrane ... See full document
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AKT signaling promotes derivation of embryonic germ cells from primordial germ cells
... serine/threonine kinase AKT, one of the major downstream effectors of PI3K, on the developmental potency of PGCs. We used transgenic mice that expressed an AKT-MER fusion protein, the kinase activity of which could be ... See full document
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Effectiveness of specific RNA mediated interference through ingested double stranded RNA in Caenorhabditis elegans
... as RNA can be absorbed through the gut and distributed to somatic tissues and the germ line ...identify C. elegans genes involved in the RNAi mechanism ... See full document
10
Cortical granule exocytosis in C elegans is regulated by cell cycle components including separase
... cortical granule trafficking was not ...cortical granule population (see Fig. S2A in the supplementary material). CAV-1::GFP appeared normal in oocytes depleted in ...cortical granule ... See full document
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