[PDF] Top 20 Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
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Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
... rapid PCR-based diagnostics, has led to greater pres- sure for diagnostic laboratories to adopt molecular biology- based diagnostic ...The LightCycler instrument is a recently developed, commercially ... See full document
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Rapid and Sensitive Method Using Multiplex Real Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
... viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent ...assays. Reverse transcriptase PCR is now recognized as a sensitive and ... See full document
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MultiCode RTx Real Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples
... clinical samples from 13 treatment-experienced patients with viral loads between 112 and 26,841 copies/ml were also tested for K65R (Table ...20 samples contained a polymorphic mutation at the binding site ... See full document
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Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms
... the samples, vor- texed and processed routinely followed by centrifuged at 2000 rpm for 7 ...DFA Respiratory Virus Screening & ID Kit, Diagnostic Hybrids, ... See full document
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Direct Detection of Respiratory Syncytial Virus, Parainfluenza Virus, and Adenovirus in Clinical Respiratory Specimens by a Multiplex Reverse Transcription PCR Assay
... RT-PCR detection of RSV in respiratory specimens closely paralleled direct antigen detection, while detection of PIV3 and detection of adenovirus were much more dissimilar from ... See full document
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... (15.58%) virus co-infections were found, including 36 ...in detection of RSV, HRV and HMPV were more than 95% and the sensitivities of the mOTNRT-PCR assay were ...three samples that were ... See full document
7
Detection of Rhinovirus, Respiratory Syncytial Virus, and Coronavirus Infections in Acute Otitis Media by Reverse Transcriptase Polymerase Chain Reaction
... NPA samples were obtained at each child’s initial ...NPA samples were obtained with a suction catheter inserted through a nostril to a depth of 4 to 8 cm, followed by gentle application of suction with an ... See full document
7
Respiratory Syncytial Virus Regulates Human MicroRNAs by Using Mechanisms Involving Beta Interferon and NF κB
... real-time PCR using miRNA-specific primer probe sets for let-7b, let-7i, or miR-30b (Applied ...by reverse transcription from 50 ng RNA with the TaqMan microRNA RT kit and sequence-specific primers (Applied ... See full document
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Unbiased Detection of Respiratory Viruses by Use of RNA Sequencing Based Metagenomics: a Systematic Comparison to a Commercial PCR Panel
... A virus-positive specimens were typed as 2009 H1N1 ...rhinovirus-positive samples, coverage of the viral genome was suf- ficient to generate full-length viral consensus ...respiratory virus. ... See full document
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Diagnostic Accuracy of Rapid Antigen Detection Tests for Respiratory Syncytial Virus Infection: Systematic Review and Meta analysis
... Respiratory syncytial virus (RSV) rapid antigen detection tests (RADT) are extensively used in clinical ...(reverse transcriptase PCR [RT-PCR], immunofluo- ... See full document
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Molecular Quantification of Respiratory Syncytial Virus in Respiratory Samples: Reliable Detection during the Initial Phase of Infection
... concentrations, samples were assayed in duplicate in a 50-l reaction mixture containing 20 l of cDNA, 25 l of 2⫻ TaqMan universal PCR master mix (PE Applied Biosystems, Foster City, CA), 900 nM ... See full document
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Comparison of Viral Isolation and Multiplex Real Time Reverse Transcription PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays
... of samples from patients with a wide age range, the mean age of the influenza virus-positive patients was 42 ...study, PCR was the only method which provided results both rapid and accurate enough ... See full document
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Viral respiratory infections in a nursing home: a six month prospective study
... residents (only one with ARI) were tested for the pres- ence of viruses in a nasopharyngeal swab. The predom- inant virus, detected in participants with ARI as well as in healthy participants, was hRV/EV (Table ... See full document
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Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... Accurate detection of respiratory viruses is important to guide antiviral therapy, prevent nosocomial spread, provide surveillance, and in some cases, decrease hospital costs and lengths of stay (1, 2, 11, ... See full document
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Bioconjugated nanoparticle detection of respiratory syncytial virus infection
... detect respiratory syncytial virus in vitro and in ...sensitive detection of viruses and thereby bridge the gap between current cumbersome virus detection assays and the ... See full document
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Respiratory Syncytial Virus (RSV) Infection in Preterm Infants and the Protective Effects of RSV Immune Globulin (RSVIG)
... To evaluate the safety and ef- ficacy of respiratory syncytial virus immune globulin (RSVIG) in the prevention of severe respiratory syncytial virus (RSV) lower respiratory tract infecti[r] ... See full document
7
microRNAs in viral acute respiratory infections: immune regulation, biomarkers, therapy, and vaccines
... the human genome, allow- ing the generation of 2588 mature miRNAs according to miRBase ...the respiratory tract and regulation of anti-viral host defense ...acute respiratory symptoms ...chronic ... See full document
7
Human rhinovirus infection in young African children with acute wheezing
... A prospective study of children aged 2 months to 5 years presenting with acute wheezing to Red Cross War Mem- orial Children’s Hospital (RCCH) from May 2004 to November 2005 (2 winter seasons) was undertaken. RCCH is a ... See full document
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Respiratory viral infection: a potential “missing link” in the pathogenesis of COPD
... acute respiratory virus infection in disease ...underlying virus-induced exacerbations remain relatively poorly elucidated and current anti-viral therapies are ... See full document
14
Comparison of Commercial Real Time Reverse Transcription PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
... field samples rather than a group of sham-inoculated boars were included in this study to determine the specificity of the PCR ...independent samples as opposed to a few dependent samples from ... See full document
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