[PDF] Top 20 Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

... CRISPR–Cas system in LAB except for ...genetic system was not functional in other LAB and still left a lox72 site, a heterologous DNA sequence after the antibiotic selection marker excised from the ... See full document

... during lactic fermentations (Liu et ...2014). Lactic acid bacteria (LAB) play a key role in food fermentations where they not only contribute to the development of the desired sensory ... See full document

... the development of plasmid expression vectors of increased stability (33, 34) and chromosomal integration systems which target specific or random loci and that are based on a variety of non-replicative or unstable ... See full document

... Korterink et al. [78] performed a systematic meta-analysis for the use of probiotics for childhood functional gastrointestinal disorders (abdominal pain, vomiting and aerophagia), which cannot be explained in terms of ... See full document

... in lactic acid bacteria (LAB), including suicide plasmids for generating gene disruptions and the development of inducible expression systems for regulated protein ...in lactic ... See full document

... flavor development take ...of Lactic acid bacteria (LAB) are responsible for the acidification in ...Starter lactic acid bacteria (SLAB) are added to the cheese to speed ... See full document

... “lactic acid”, has been proved to preserve important constituents present in milk, in a relatively more stable (Mehaia, ...human. Lactic acid also improves the usage of minerals like calcium ... See full document

... of bacteria from milk of domestic animals ...probiotic bacteria used in food products to date have been of human or dairy origin as these probiotics possess the immunomodulatory effect and they are able to ... See full document

... The information gathered in this study may be used to enhance the nutritional identity for the Malaysian stingless bee honey, such as for product labelling and marketing along with the possibility of promoting a ... See full document

... the lactic fermentation plays a key role in safety and acceptability of these products in tropical climates (Haggblade and Holzapfel, ...fungi, bacteria and yeasts on plant materials are little known ... See full document

... produce acid during manufacture and also contribute to the ripening ...of acid, but they contribute more during ripening ...flavor development by LAB including metabolism of lactose, lactate and ... See full document

... the development of bio-waste ...high lactic acid yield, high acid tolerance, and their ability to be metabolically engineered ...of lactic acid (> 95%), differences were not ... See full document

... optimum development at temperatures between 25°C and 40°C, the genus Leuconostoc between 18°C-30°C, the Lactococcus between 25°C and 32°C , Lactobacillus mesophilic 30 or 35°C, Lactobacillus thermophilic between ... See full document

... BIMO-CF performance on process fermentation is greatly influenced by time fermentation. The more time fermentation then BIMO CF opportunity to disconnect peptide bond on the protein becomes acidic lactate is getting ... See full document

... The obtained values are in close relation with the induction process of sporulation, the total number of spores in mixed biofilms reaches the value of (0,4 ± 0,0). 10 2 cfu/ml for B. subtilis 170 and E. coli K-12 1655 ... See full document

... LAB identification using kit API CHL 50 (Biomerieux, France) was carried out to determine the type of LAB species. Testing was carried out by isolating one ose of LAB which placed into 10 ml of MRSB medium and then ... See full document

... interspaced short palindromic repeats (CRISPR) and associated sequences (cas) have shown promise for phage resistance. CRISPR-Cas systems consist of arrays of short DNA repeats interspaced by hypervariable sequences, ... See full document

... restored activity (37). Additional studies reported that deletion of ltaS correlates with lower levels of peptidoglycan hydrolases in S. aureus (38) and substantiated direct association between LTA and autolysins in the ... See full document

... The term probiotic is mostly related to lactic acid bacteria ( Lactobacillus and Bifidobacterium Sp.),.. 110.[r] ... See full document

... Lactic acid bacteria also produce metabolites and some of them have antibacterial activity including organic acids (lactic acid, acetic acid, propionic acid, sorbic ... See full document