[PDF] Top 20 Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle
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Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle
... Serum Ig ELISA with M. bovis LAM-enriched antigen. An ELISA with lipo- arabinomannan (LAM)-enriched antigen was performed as described previously (18, 19). Briefly, Immulon II 96-well microtiter plates (Dynatech, ... See full document
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Development of an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Ringworm Infection in Cattle
... in-house enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of ringworm infection in ...135 cattle with ringworm infection, as confirmed by direct microscopy, ... See full document
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Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows
... ovine tuberculosis (TB) caused by Mycobacterium bovis is a major economic problem in many countries, and control of this chronic disease in domestic livestock has often proved diffi- ...from cattle in a ... See full document
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Evaluation of an IgG Enzyme Linked Immunosorbent Assay as a Serological Assay for Detection of Mycoplasma bovis Infection in Feedlot Cattle
... dairy cattle in North America and Europe (19–21), but this is the first study to our knowledge looking at the seroepidemiology of ...dairy cattle but no analysis of the prevalence of infec- tion in beef ... See full document
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Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis.
... the detection of anti-Neospora antibodies in experimentally inoculated ...the evaluation of a serodiagnostic test, since antibody responses of experimentally inoculated animals may differ from those of ... See full document
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Development of a New Indirect ELISA Method for Detection of Anti-Tuberculosis Antibodies in Human Serum
... Indirect Enzyme-linked immunosorbent Assay (ELISA) was established to detect serum antibodies against Mycobacterium ...M. tuberculosis Bacilli, and Mycobacterium bovis Bacillus Calmette ... See full document
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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle
... competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a ... See full document
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Interpretation of a Commercial Bovine Paratuberculosis Enzyme-Linked Immunosorbent Assay by Using Likelihood Ratios
... same cattle have been used in the evaluation of other diagnostic tests for paratuberculosis (22– ...infected cattle and do not include M. paratuberculosis-infected cattle that test negative by ... See full document
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Use of Antigen-Specific Interleukin-2 To Differentiate between Cattle Vaccinated with Mycobacterium bovis BCG and Cattle Infected with M. bovis
... novel bovine interleukin-2 (IL-2) enzyme-linked immunosorbent assay (ELISA) for the measurement of antigen-specific IL-2 in cattle naturally infected with Mycobacterium bovis and ... See full document
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Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme linked immunosorbent spot assay for detection of mycobacteria
... spot assay. The capture spot ELISA was adapted from the assay of Spira and Scharff (15) for the purpose of capturing and visualizing a single ...and development product) was ...M. tuberculosis ... See full document
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Development of an Indirect Tams1 Enzyme-Linked Immunosorbent Assay for Diagnosis of Theileria annulata Infection in Cattle
... ELISA using sheep anti-bovine IgG1 and IgG2 and mouse anti-bovine IgG1. Sheep anti-bovine IgG1 and IgG2 were purchased from ICN (Costa Mesa, Calif.). Horseradish peroxidase was purchased from ... See full document
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Humoral immune response in human tuberculosis: immunoglobulins G, A, and M directed against the purified P32 protein antigen of Mycobacterium bovis bacillus Calmette Guérin
... Serodiagnosis of pulmonary tuberculosis in Argentina by enzyme-linked immunosorbent assay ELISA of IgG antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein de[r] ... See full document
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Immunoglobulin G, A, and M Responses in Serum and Circulating Immune Complexes Elicited by the 16-Kilodalton Antigen of Mycobacterium tuberculosis
... The cytosol fraction of the sonicate antigen of M. tuberculosis H37Rv was passed through gel filtration chromatography using Ultrogel ACA34 matrix (Pharmacia, Uppsala, Sweden). Initially, 150 mg of the cytosol ... See full document
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Use of an enzyme linked immunosorbent assay for detection of Treponema hyodysenteriae infection in swine
... The mean ELISA titer of naturally infected pigs without clinical signs was not significantly P < 0.05 different from the mean ELISA titers of two groups of experimentally infected pigs T[r] ... See full document
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Sensitive Enzyme Immunoassay for Hepatitis B Virus Core Related Antigens and Their Correlation to Virus Load
... this assay, HCV core antigen was released from virus virion by SDS pretreatment prior to ...this detection system to HBV core and e ...the assay to detect HBcrAg with mutations at one or more ... See full document
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Enzyme linked immunosorbent assay for detection of serum antibodies to Pasteurella haemolytica cytotoxin (leukotoxin) in cattle
... The strongest correlation was between the ELISA cytotoxin antibody response and cytotoxin neutralization titers, whereas the lowest was between the ELISA cytotoxin antibody response and [r] ... See full document
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Enzyme linked immunosorbent assay for detection of measles antibody
... Detection of er, the mean value for a particular titer was IgG antibodies for measles virus by enzyme-linked immunosorbent assay ELISA.. This variation is due in part to the discrete two[r] ... See full document
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Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus.
... the detection of DEN antibodies are not ...dipstick enzyme-linked immu- nosorbent assay (ELISA) was ...enhancer, enzyme-conjugated anti-human IgG and IgM antibody, and ...Total ... See full document
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Evaluation of enzyme linked immunosorbent assay for the serodiagnosis of amebiasis
... When the results of ELISA were compared with those of the IFA test performed on a total of 514 sera from patients with clinically proven and suspected amebiasis, positive findings were 3[r] ... See full document
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Use of Enzyme Linked Immunosorbent Assay and Dipstick Assay for Detection of Strongyloides stercoralis Infection in Humans
... the enzyme-linked im- munosorbent assay (ELISA), which detects serum immuno- globulin G (IgG) against a crude extract of infective ...dipstick assay (4, 13, ... See full document
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