[PDF] Top 20 Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples

... Background: Hookworm infection is a major concern in sub-Saharan Africa, particularly in children and pregnant ...condition. Hookworm disease is one of the Neglected tropical diseases (NTDs) that are ... See full document

... Chaichontat Sriworarat 1 , Atchara Phumee 2 , Mathirut Mungthin 3 , Saovanee Leelayoova 3 and Padet Siriyasatien 2,4* Abstract Background: Leishmaniasis is a neglected tropical disease that is caused by an obligate ... See full document

... Conclusions A visualized closed-tube LAMP method was established utilising microcrystalline wax-dye capsules, which can deliver highly sensitive DNA fluorescence dye to the reaction mixture following amplification ... See full document

... the development of a loop-mediated isother- mal amplification (LAMP) assay for histoplasmosis, which pro- vides an affordable method of molecular identification that can be performed and ... See full document

... suspected infection are too weak to undergo invasive ...qPCR detection on clinical samples showed that they had good concordance; besides simplicity, however, the detection limit and speed of ... See full document

... Blood samples were collected from the vena orbitalis posterior plexus blood at 1-9 day post infection ...blood samples DNA was extracted by using the three above-mentioned DNA extraction ...blood ... See full document

... novel technique that can possibly be used for rapid diagnosis of BKV infection not only in laboratories but also in an outpatient clinic ...BKV detection, which might take 2 to 3 days or longer to ... See full document

... However, compared to EB with strong mutagen, Gold- view has relatively fewer mutations and higher safety. Particularly, similar clinical symptoms in infections caused by EV71 and CVA16 with high similarity in nucleotide ... See full document

... The high specificity of the RT-LAMP assay was also exhibited in 40 clinical samples, including seven CVA16-positive sam- ples. The RT-LAMP assay is simple and easy to perform. The assay employs regular primers ... See full document

... ISO technique for the detection of the ...the detection limit assessed in dairy substrates was higher if compared to ...the samples was identified, while after 24 h, only few sam- ples ... See full document

... To assess the quality of LAMP assay, a panel of nucleic acid extracts and test kits were dispatched to 28 separate testing health agency laboratories in the endemic areas at the provincial and county levels where ... See full document

... earlier detection of infection results in earlier treatment and consequently, earlier recovery ...extraction technique that requires min- imal equipment and can produce sufficient DNA within a short ... See full document

... thermal amplification (RT-LAMP) assay for the detection of norovirus (NoV) genomes targeting from the C terminus of the RNA-dependent RNA polymerase gene to the capsid N-terminal/shell domain ...the ... See full document

... 1.2.8. Loop-mediated isothermal amplification (LAMP) Molecular amplification-based techniques are one of the most precious implements in all life science fields like in diagnosis of ... See full document

... transcription loop-mediated isothermal amplification (RT-LAMP) assay for human enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) infection was further ...The detection limits ... See full document

... Methods: LAMP specificity was evaluated using a panel containing DNA from a range of different organisms. Sensitivity was evaluated by preparing 10-fold serial dilutions of N. caninum tachyzoites and comparing the ... See full document

... the evaluation test with clinical specimens obtained from the outbreak in Angola, the RT-LAMP assay using Musoke lineage-specific primers showed good performance with regard to concordance and specificity, even ... See full document

... the detection of prostate-specific antigen mRNA in K562 cells ...of infection by using throat washes and combined throat and nasal swab ...The evaluation of the RT-LAMP assay for detection of ... See full document

... loa infection has had a severely negative impact on control (MDA) programs for onchocerciasis and lym- phatic filariasis in West and Central Africa, there is a consensus that point-of-care diagnostics are needed ... See full document

... (35). In addition, LAMP has been shown to be tolerant against naturally occurring inhibitors in biological samples (16). The capripox LAMP primers used in this study were designed using the most commonly used ... See full document