[PDF] Top 20 Diagnosis of Extrapulmonary Tuberculosis by Smear, Culture, and PCR Using Universal Sample Processing Technology
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Diagnosis of Extrapulmonary Tuberculosis by Smear, Culture, and PCR Using Universal Sample Processing Technology
... AFB smear-positive but culture-negative speci- mens were determined to be negative by PCR; we have no explanation for this except for the nonuniform distribution of bacilli in the aliquots ... See full document
6
A comprehensive comparision of mpb64 based pcr assay versus microscopy and culture in the diagnosis of clinically suspected cases of extrapulmonary tuberculosis
... Pulmonary tuberculosis is increasing worldwide. The diagnosis of EPTB is always ...early diagnosis and prompt initiation of appropriate ...the PCR using MPB64 primer for early ... See full document
5
Utility of Universal Sample Processing Methodology, Combining Smear Microscopy, Culture, and PCR, for Diagnosis of Pulmonary Tuberculosis
... inhibited PCR, and with culture as the gold standard, the sensitivity of IS6110 PCR was ...the culture positivity rate of ⬃ 56% (322/571) to be lower than that of USP smears, at 60% (343/571), ... See full document
6
Early Diagnosis of Extrapulmonary Tuberculosis by a New Procedure Combining Broth Culture and PCR
... The diagnosis of extrapulmonary tuberculosis is difficult because of the paucibacillary nature of these ...a culture-enhanced PCR assay combining a preliminary step of broth ... See full document
6
Xpert MTB/RIF: a New Pillar in Diagnosis of Extrapulmonary Tuberculosis?
... of tuberculosis (TB) cases in India are estimated to have extrapulmonary disease, and due to a lack of diagnostic means, they often remain ...Mycobacterium tuberculosis and multidrug resistance is a ... See full document
6
Rapid Diagnosis of Extrapulmonary Tuberculosis by Ligase Chain Reaction Amplification
... the diagnosis of extrapulmonary tuberculosis, the LCx Mycobacterium tuberculosis Assay from Abbott Laboratories, was ...M. tuberculosis Assay were compared with those from ... See full document
6
Assessment by Meta Analysis of PCR for Diagnosis of Smear Negative Pulmonary Tuberculosis
... of PCR for the diagnosis of smear-negative pulmonary tuberculosis (SPT) and to identify factors that account for differences in the diagnostic accuracy of different ...of PCR in ... See full document
8
Evaluation of in house PCR for diagnosis of smear negative pulmonary tuberculosis in Kampala, Uganda
... confirm diagnosis of TB, follow-up at 2 months (window 8 to 16 weeks) was done for PCR-positive/culture negative as well as PCR- negative/culture negative study participants and the ... See full document
8
Comparative Evaluation of Several Gene Targets for Designing a Multiplex PCR for an Early Diagnosis of Extrapulmonary Tuberculosis
... plex PCR, with the exception of the different primer pair con- ...2) using 1 μL of purified M. tuberculosis DNA (1 ng/mL) to choose an appropriate ratio for mpb64:IS6110, which showed the clear ... See full document
9
Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience
... than culture-based identification. There- fore, PCR-based methods promise to be very effective for the diagnosis of fungal ocular infections in the clinical ...the diagnosis. PCR may be ... See full document
8
Novel Multipurpose Methodology for Detection of Mycobacteria in Pulmonary and Extrapulmonary Specimens by Smear Microscopy, Culture, and PCR
... easy universal sample processing and diagnostic method- ology applicable to any type of clinical specimen for the labo- ratory diagnosis of ...sensitive smear microscopy for AFB ... See full document
6
Accuracy of a new rapid antigen detection test for pulmonary tuberculosis
... Objectives: Tuberculosis (TB) is a major problem in the ...Mycobacterium tuberculosis (MT) in the proper samples. While smear doesn’t have enough sensitivity, culture and PCR are ... See full document
5
Poor Performance of Universal Sample Processing Method for Diagnosis of Pulmonary Tuberculosis by Smear Microscopy and Culture in Uganda
... direct smear microscopy ...the universal sample processing (USP) method for preparation of pulmonary and extrapulmo- nary specimens for smear microscopy, mycobacterial culture, ... See full document
5
Rapid Diagnosis of Extrapulmonary Tuberculosis by PCR: Impact of Sample Preparation and DNA Extraction
... M. tuberculosis culture negative but PCR positive are truly false positive or are actu- ally detecting ...conventional culture, there were five PCR- positive, culture-negative ... See full document
7
Diagnosis of Tuberculosis by GeneXpert MTB/RIF Assay Technology: A Short Review
... and extrapulmonary samples of the patients showing symptoms of tuberculosis ...and extrapulmonary samples include pleural fluid, lymph node biopsy, disc material, ascitic fluid, cerebrospinal fluid, ... See full document
5
Diagnosis of extrapulmonary tuberculosis by Gen Probe amplified Mycobacterium tuberculosis direct test
... M. tuberculosis complex rose to 100% in the case of body fluids and aspirates and to ...inhibitors. Using the NALC-NaOH decontam- ination protocol, we were able to reduce inhibition by approx- imately 80% ... See full document
5
New synthetic lipid antigens for rapid serological diagnosis of tuberculosis
... assay using these sets, we validated it through blinded testing of a further 249 samples pro- vided from TDR without any accompanying data (Table ...analysed using cut-off values set for the first 100 ... See full document
28
Occurrence of Overlooked Zoonotic Tuberculosis: Detection of Mycobacterium bovis in Human Cerebrospinal Fluid
... by smear microscopy and the prolonged time taken by culture have been overcome by molecular techniques (4, 7, 9, 20, 22, ...M. tuberculosis and ...M. tuberculosis from ...a sample would ... See full document
7
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... of using a cough of 2 weeks or more for screening and microscopy for ...screening using symptoms and X-ray results; and diagnosis by smear and culturing with the GeneXpert assay to confirm ... See full document
8
Recurrent Intestinal Obstruction in a Patient with Selective IgA Deficiency
... bacilli smear, Mycobacteria tuberculosis culture and Mycobacteria tuberculosis polymerase chain reaction were once again ...ileum tuberculosis culture, stool for Mycobacteria ... See full document
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