[PDF] Top 20 Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription.
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Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription.
... HIV-1 RT purification. The procedures developed by Muller et al. (18) were used to extract HIV-1 RT expressed in Escherichia coli containing plasmids coding for the RT subunits (pRT66 and pRT51), with the ... See full document
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Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys).
... from reverse transcription, mutant proviral genomes were constructed in which the PBS region was altered so that proviruses which were generated as a result of reverse transcription would pos- ... See full document
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Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys).
... Characterization of transiently produced virions. All PBS- replacement viruses showed reduced replication rates and could not be stably maintained because of reversion. In order to study the mutant viruses in more ... See full document
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Human Immunodeficiency Virus Type 1 (HIV-1) Viral Protein R (Vpr) Interacts with Lys-tRNA Synthetase: Implications for Priming of HIV-1 Reverse Transcription
... ceptor type II complex (56, 72) are cellular proteins that were previously found to interact with ...the effects of the N-terminal region is not without ... See full document
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Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell.
... mutant virus (HIV D nef) carrying a frameshift mutation in the nef gene (see Materials and Methods) and the wild-type virus ...for virus pro- duction by measuring p24 in culture supernatants ... See full document
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Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Target Distinct Phases of Early Reverse Transcription
... affect reverse transcription; Nef (1, 50) and Vif (11, 12, 42, 51) seem to affect reverse transcription during virus particle formation or uncoating or by affecting RNA structure ... See full document
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Mutations in Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Zinc Fingers Cause Premature Reverse Transcription
... for 1 h at 37°C in a volume of 100 l according to the manufacturer’s ...with 1 l of 25 mg/ml yeast tRNA (a precipitation ...and 1 U/ l RNaseOUT ...with 1 cycle consisting of 15 ... See full document
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Human Immunodeficiency Virus Type 1 N-Terminal Capsid Mutants That Exhibit Aberrant Core Morphology and Are Blocked in Initiation of Reverse Transcription in Infected Cells
... of reverse transcription as well as for two-LTR circular ...during reverse transcription. In contrast, the samples from mutant virus-infected cells showed small amounts of (⫺) SSDNA and ... See full document
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Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription
... in reverse transcription was also seen in endogenous reverse transcription ...of reverse transcription, al- though its exact role in this process has not been ...the ... See full document
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Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription
... endogenous reverse transcription (NERT) (14, 52–55). In this procedure HIV-1 virions are exposed to buffer containing high concentrations of deoxynucleoside triphosphates (dNTPs) and ...early ... See full document
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Association of Human Immunodeficiency Virus Type 1 Vif with RNA and Its Role in Reverse Transcription
... Thus far, it is unclear whether Vif has specificity for the HIV-1 genomic RNA. However, we have provided evidence that Vif affects viral nucleic acid components to influence the initiation of reverse ... See full document
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Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
... HIV-1 reverse transcription. First, viruses with the wild-type RT derived from pHXB2(His-AC-TGT) replicated more slowly than viruses derived from pHXB2(His- AC-GAC) ...reverse ... See full document
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The Role of Nucleocapsid and U5 Stem/A-Rich Loop Sequences in tRNA3Lys Genomic Placement and Initiation of Reverse Transcription in Human Immunodeficiency Virus Type 1
... simian virus 40-based vector which contains full-length wild-type HIV-1 proviral DNA (27, 41) and was a gift from ...(Fig. 1) were verified by direct sequence analysis of the SpeI-SalI ... See full document
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Defective Replication in Human Immunodeficiency Virus Type 1 When Non-Primers Are Used for Reverse Transcription
... prime reverse transcription. We next determined the ability of each type of virion to carry out the initiation of reverse ...primer tRNA/viral RNA template in an in vitro ... See full document
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Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... plasma human immunodeficiency virus type 1 (HIV-1) RNA levels has become an important tool for identifying individuals likely to benefit from antiretroviral ther- apy (12, 15, ... See full document
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Effects of Varying Sequence Similarity on the Frequency of Repeat Deletion during Reverse Transcription of a Human Immunodeficiency Virus Type 1 Vector
... to human immunodeficiency virus type 1 (HIV-1) diversity, with homol- ogous recombination being more frequent than nonhomologous ...the effects of various extents of ... See full document
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Human Immunodeficiency Virus Type 1 Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex
... the virus replication level of the molecular mechanisms that are involved in assembly, maturation, uncoating, and the early stages of reverse transcription remains ...the virus life cycle, the ... See full document
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Molecular Mechanisms by Which Human Immunodeficiency Virus Type 1 Integrase Stimulates the Early Steps of Reverse Transcription
... the initiation mode, we carried out the reaction using 20 nM of RT, which was within the linear range of the assay, in the presence or absence of various concentrations of IN ...5 initiation product ... See full document
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A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription.
... than reverse transcriptase are involved in the control of HIV-1 reverse ...dation, tRNA packaging and annealing at the PBS (6, 46), and strand transfer in cooperation with reverse ... See full document
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Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin
... in virus- specific complexes early after infection, but it was absent in cRTCs analyzed at later time points and in nuclear RTCs (nRTCs) [4,7] The composition of the HIV-1 nPICs is still ...or ... See full document
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