[PDF] Top 20 Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles.
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Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles.
... 6929 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest The Li genes of two human papillomavirus type 16 HPV16 isolates derived from condylomata acuminata used to express t[r] ... See full document
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Nasal Immunization of Mice with Human Papillomavirus Type 16 (HPV-16) Virus-Like Particles or with the HPV-16 L1 Gene Elicits Specific Cytotoxic T Lymphocytes in Vaginal Draining Lymph Nodes
... HPV-16 L1 VLPs under anesthesia ...(titer, 16) was observed in vaginal secretions from the anesthetized mice immunized with HPV-16 L1 DNA combined with CT, but the titer obtained was ... See full document
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Interactions between Papillomavirus L1 and L2 Capsid Proteins
... The human papillomavirus (HPV) capsid consists of 360 copies of the major capsid protein, L1, arranged as 72 pentamers on a T ⴝ 7 icosahedral lattice, with substoichiometric amounts of the minor ... See full document
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Identification of Human Papillomavirus Type 16 L1 Surface Loops Required for Neutralization by Human Sera
... on human papillomavirus (HPV) virions required for type-specific neutralization by human sera remain poorly ...hybrid virus-like particles (VLPs) were used to adsorb ... See full document
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Analysis of Modified Human Papillomavirus Type 16 L1 Capsomeres: the Ability To Assemble into Larger Particles Correlates with Higher Immunogenicity
... launched virus-like particle (VLP)-based prophylactic vaccines against infection with human papillomavirus types 16 and 18 (HPV-16 and HPV-18), which are causative agents of ... See full document
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Self-assembly of in vitro-translated human papillomavirus type 16 L1 capsid protein into virus-like particles and antigenic reactivity of the protein.
... the L1 protein alone is sufficient for formation of ...the L1 protein of HPV, can assemble into VLPs in vitro in simple buffer solutions containing low concentrations of cal- cium and magnesium salts (17, ... See full document
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Cyclophilins Facilitate Dissociation of the Human Papillomavirus Type 16 Capsid Protein L1 from the L2/DNA Complex following Virus Entry
... C virus (HCV), depend on cyclophilin function for comple- tion of their life ...for efficient RNA replication (21, 65, ...to efficient entry into host ... See full document
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Characterization of a Major Neutralizing Epitope on Human Papillomavirus Type 16 L1
... with human papillomavirus type 16 (HPV-16) is strongly associated with the develop- ment of cervical ...protein, L1, have not been well characterized, although three neutralizing ... See full document
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The L1 Major Capsid Protein of Human Papillomavirus Type 16 Variants Affects Yield of Virus Like Particles Produced in an Insect Cell Expression System
... this virus ge- notype, as has been observed for HPV-5 ...from virus strains isolated in Senegal, Algeria, and the ...the L1 proteins from these three ... See full document
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Human papillomavirus type 16 variant lineages in United States populations characterized by nucleotide sequence analysis of the E6, L2, and L1 coding segments.
... the L1 ORF, is the target of neutralizing antibody responses (20, 40, 45, 57, 58, 59), and naturally occurring L1 antibodies react almost exclu- sively with conformation-dependent epitopes ...which ... See full document
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Gene Transfer Using Recombinant Rabbit Hemorrhagic Disease Virus Capsids with Genetically Modified DNA Encapsidation Capacity by Addition of Packaging Sequences from the L1 or L2 Protein of Human Papillomavirus Type 16
... into virus-like particles (VLPs) with different cell ...disease virus (RHDV) with sequences which are expected to be sufficient to confer DNA packaging and gene transfer properties to the ... See full document
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Prevalence of antibodies to human papillomavirus (HPV) type 16 virus-like particles in relation to cervical HPV infection among college women.
... a self-administered questionnaire that elicited informa- tion on demographic characteristics and sexual ...consensus L1 primers ...biotin-labeled, type-specific HPV oligonucleotide ...the ... See full document
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Enhancement of Capsid Gene Expression: Preparing the Human Papillomavirus Type 16 Major Structural Gene L1 for DNA Vaccination Purposes
... 6b L1 gene, as well as the L1 (and L2) genes of other papillomaviruses, can be expressed in cells for which HPV L1 expression is not ...of papillomavirus capsid gene ...the ... See full document
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Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells.
... producing virus-like particles (VLPs) void of DNA by synthesis of the capsid proteins in eukaryotic expres- sion systems (17, 21, 30, 37, ...protein L1 alone or L1 and the minor capsid ... See full document
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Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions.
... HPV 16 VLP of L1 proteins synthesized in ...HPV 16 VLP, while anti-HPV 16 antibodies (Camvir 6 and Camvir 7) reacted to HPV 16 VLP but not to HPV ...HPV 16, and HPV 18 virions ... See full document
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Nasal Immunization of Mice with Human Papillomavirus Type 16 Virus-Like Particles Elicits Neutralizing Antibodies in Mucosal Secretions
... comprising L1 and L2, surrounding the bovine papilloma- virus type 1 genome, designated HPV16 {BPV1}, were generated as previously described ...B virus: HBc antigen [25, 43]) were used ... See full document
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A Direct Comparison of Human Papillomavirus Type 16 L1 Particles Reveals a Lower Immunogenicity of Capsomeres than Viruslike Particles with Respect to the Induced Antibody Response
... (PyV)-like particles and HPV16 VLPs has also been reported previously (22, 52, ...different L1 assembly forms can presumably be explained by the fact that T cells are not directly dependent on ... See full document
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Self-assembly of human papillomavirus type 1 capsids by expression of the L1 protein alone or by coexpression of the L1 and L2 capsid proteins.
... Cells infected with vaccinia virus vectors expressing only the major capsid protein Li were also analyzed by cesium chloride centrifugation.. vac-MJlLl produced a large amount of Li prot[r] ... See full document
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Chimeric L1-L2 Virus-Like Particles as Potential Broad-Spectrum Human Papillomavirus Vaccines
... of L1 was chosen for insertion to display the L2 peptide on the surface of the assembled chimeric BPV1 VLP ...anti-HPV L1 antisera that might obscure detection of low-titer anti-HPV16 L2 ... See full document
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Mechanisms of Human Papillomavirus Type 16 Neutralization by L2 Cross-Neutralizing and L1 Type-Specific Antibodies
... the L2 MAb had too low an avidity to immunoprecipitate L1/L2 com- plexes, we also examined the ability of these two MAb to immunoprecipitate pseudovirion assembly intermediates and/or immature ... See full document
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