[PDF] Top 20 The gene encoding the nonstructural protein of B19 (human) parvovirus may be lethal in transfected cells.
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The gene encoding the nonstructural protein of B19 (human) parvovirus may be lethal in transfected cells.
... The observation of transient expression of viral proteins including capsid proteins when nearly full-length B19-conwas used [data not shown] indicated that the effect of B19 gene product[r] ... See full document
6
A cytotoxic nonstructural protein, NS1, of human parvovirus B19 induces activation of interleukin-6 gene expression.
... oma cells or after the resection of the hyperplastic lymph node, resulting in dramatic reduction of IL-6 production ...IL-6 may contrib- ute, presumably as a result of enhancement of production of ... See full document
7
The 11-Kilodalton Nonstructural Protein of Human Parvovirus B19 Facilitates Viral DNA Replication by Interacting with Grb2 through Its Proline-Rich Motifs
... 11-kDa protein associates with Grb2 during ...(S1) cells or B19V-infected ...Grb2 protein (at 2 M) with WT 11-kDa at different concentrations, as ... See full document
15
Genome Replication and Postencapsidation Functions Mapping to the Nonstructural Gene Restrict the Host Range of a Murine Parvovirus in Human Cells
... range may be an exclusive phenom- enon of the particular system of transformed nervous cells used in this report and are thus of poor biological interest for other viral-host interactions in the ...in ... See full document
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Transcriptional transactivation of parvovirus B19 promoters in nonpermissive human cells by adenovirus type 2.
... E1A protein has also been known to play a vital role in AAV replication (7, ...E1A protein transactivation include those of the herpes simplex virus type 1 gpD and thymidine kinase genes (13, 54), the ... See full document
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Human parvovirus B19 nonstructural protein NS1 enhanced the expression of cleavage of 70 kDa U1-snRNP autoantigen
... competent cells, which were obtained from Life Technologies (Life Technologies, Carlsbad, California, ...of B19- NS1 was abolished by single substitutions of amino acids within the NTP-binding domain ...of ... See full document
10
Human Parvovirus B19 Nonstructural Protein (NS1) Induces Cell Cycle Arrest at G1 Phase
... 293T cells were performed with FuGene 6 (Roche Diagnostics, Indianapolis, ...UT7/Epo-S1 cells were performed with “amaxa” electroporation gene transfer tool (Amaxa GmbH, Berlin, ...brief, ... See full document
7
Human Parvovirus B19 Nonstructural (NS1) Protein Induces Apoptosis in Erythroid Lineage Cells
... erythroid-lineage cells by human parvovirus B19 is characterized by a gradual cytocidal ...the nonstructural (NS1) protein of the virus in cytotoxicity, but the mechanism underlying the ... See full document
11
The 3' untranslated region of the B19 parvovirus capsid protein mRNAs inhibits its own mRNA translation in nonpermissive cells.
... the B19 virus genome con- tains the sequence coding for the NS1 nonstructural protein, and the 39 end harbors the sequences coding for the two struc- tural proteins, VP1 and its ... See full document
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Apoptosis of Liver-Derived Cells Induced by Parvovirus B19 Nonstructural Protein
... fusion protein with ...the gene of interest is cloned and expressed under the control of the ecdysone response element and the plasmid pVGRXR, which expresses the modified ecdysone receptor and the ... See full document
8
Parvovirus B19 Infection of Human Primary Erythroid Progenitor Cells Triggers ATR-Chk1 Signaling, Which Promotes B19 Virus Replication
... uninfected cells, they localized to the B19V replication center in infected cells ...to cells in the mock group, those infected with B19V expressed ⬃ 4-fold more p-Chk1 and p-Chk2, as determined ... See full document
10
Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells
... in transfected cells appeared differ- ent from its distribution in viral infected ...recombinant protein expressed in transfected cells, all the cell lysates were subjected to SDS-PAGE, ... See full document
9
Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments.
... However, when anti-IgM antibodies were added to plasma taken during the convalescent phase and the two were used together to precipitate particulate material from plasma taken during the[r] ... See full document
10
Role of Erythropoietin Receptor Signaling in Parvovirus B19 Replication in Human Erythroid Progenitor Cells
... GFP-expressing cells were assessed by flow cytometry ...GFP-expressing cells of the Retro-GFP and Retro-EpoR groups were selectively ...in cells of the indicated treatment groups were quantified by ... See full document
12
Caspase Cleavage of the Nonstructural Protein NS1 Mediates Replication of Aleutian Mink Disease Parvovirus
... of parvovirus nonstructural ...and may have active roles in viral ...ADV may be ...infection may constrain fully permissive replication, and this may allow viral persistence at a ... See full document
8
Structural and nonstructural proteins of a rabbit parvovirus.
... In the case of RPV, the F and G polypeptides do not seem to be the products of proteolytic cleavage of the structural proteins, because i the molecular weights of the F and G polypeptide[r] ... See full document
7
Parvovirus B19: A Cause of Sepsislike Syndrome in an Infant
... evidence of atypical lymphocytes or abnormal red blood cell morphology. Biochemistry testing revealed an aspartate aminotransferase level of 57 U/L (normal range: 22–63 U/L), an alanine aminotransferase level of 38 U/L ... See full document
6
Characterization of the Early Steps of Human Parvovirus B19 Infection
... pH, CQ has the ability to destabilize endocytic membranes, lead- ing to vesicle swelling. The intense lysosomal dysfunction caused by CQ results in a deficient transfer of cargo to the degradative lysosomes, promoting ... See full document
11
Purification and characterization of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus.
... RAD3 protein, which, like the ADV NS-1 polypeptide, has a type A consensus nucleotide binding sequence and possesses helicase and ssDNA-depen- dent ATPase ...RAD3 protein, replacing the invariant positively ... See full document
8
Chronic hepatitis caused by persistent parvovirus B19 infection
... which parvovirus B19 induces liver pathology and failure remains ...virus B19 is able to enter hepatocytes through binding to the P antigen (globoside) [19], although hepatocytes are assumed to be ... See full document
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