[PDF] Top 20 Enzyme linked immunosorbent assay for Escherichia coli heat stable enterotoxin

... The sensitivity of an enzyme-linked immunosorbent assay ELISA to detect pure native Escherichia coli heat-stable toxin ST and to identify ST-producing strains among clinical isolates was[r] ... See full document

... The gene for Escherichia coli heat-stable enterotoxin type Il ST11 was fused to the genes for protein A from Staphylococcus aureus and Pi-galactosidase in two different expression system[r] ... See full document

... Thus, although the measured concentration of STa obtained by ELISA could depend upon the monoclonal antibody used in the assay, any one of these antibodies was suitable for screening iso[r] ... See full document

... Heat-stable enterotoxin ST-producing enterotoxigenic Escherichia coli ETEC can be identified by a variety of assays, including the suckling mouse assay SMA, radioimmunoassay RIA, polyclo[r] ... See full document

... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ... See full document

... The specificity of the reaction was determined by using controls consisting of i wells not coated with antigen, ii human serum containing no detectable anti-CFA/I antibody, and iii wells[r] ... See full document

... control, Escherichia coli cells extract was di- luted in same dilution and added to the other half of the ...each assay step with 150 μl of PBS-T (Phosphate-buffered saline (PBS) with ... See full document

... Similarly, in outbreak C Table 4, associated with a VTEC 0157:H7 strain expressing VT1 and VT2, no antibody was detected in a serum specimen from the index patient with HUS obtained 13 d[r] ... See full document

... Serum immunoglobulin G antibodies to purified heat-labile enterotoxin LT from human LTh and porcine LTp Escherichia coli strains and cholera enterotoxin CT were measured by an enzyme-lin[r] ... See full document

... The recombinant DNA-derived Treponema pallidum membrane protein TmpA, purified from Escherichia coli K-12, was used in an enzyme-linked immunosorbent assay ELISA to evaluate its suitabil[r] ... See full document

... A convenient and sensitive enzyme-linked immunosorbent assay ELISA for the STb heat-stable enterotoxin of Escherichia coli was developed and used to quantify STb production by strains wi[r] ... See full document

... It is of interest that the strains of Shigella sonnei and Shigella flexneri used in this study were negative in both the RELISA and the cytotoxicity assay Table 2, but other investigator[r] ... See full document

... coli LT in whole In comparing the GM1 ELISA with the previcultures of bacteriaa ously described antiserum ELISA 14 and the Y, adrenal cells Y1 adrenal cells 9, all seem to be acceptable [r] ... See full document

... The straih previously reported to produce STaT that was negative ih the GM1-ELISA was also negative in the infant mouse test even when concentrated culture filtrate was tested.. enteroco[r] ... See full document

... Previously described GM1 ganglioside enzyme-linked immunosorbent assays GM1-ELISA for the detection of Escherichia coli heat-labile enterotoxin LT showed sensitivity equal to the Y-1 adr[r] ... See full document

... We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli ETEC expressing colonization factor a[r] ... See full document

... The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E.. coli enterotoxin but requires neither radioactive reagents n[r] ... See full document

... Of affected babies, 15 had toxigenic Escherichia coli or heat-labile E coli toxin in feces de-.. tected by enzyme-linked immunosorbent assay.[r] ... See full document

... All the cultures of the strains previously shown to produce LT had a significant enterotoxin titer as determined with the GM1-AP-ELISA and also.. the cultures to a final concentration of[r] ... See full document

... A modification of the enzyme-linked immunosorbent assay for a sensitive and rapid visual detection of heat-labile enterotoxins from Escherichia coli and Vibrio cholerae is described.. Sm[r] ... See full document