[PDF] Top 20 Epstein-Barr Virus LF2: an Antagonist to Type I Interferon

... inhibit type I IFN production, 293T cells were trans- fected with IRF7, IFN- ␣ 6 promoter-directed luciferase re- porter, and a transfectional control Renilla luciferase reporter along with individual EBV ... See full document

... of I ORF deletion mutant. A mutant virus lacking the I ORF within the N gene was constructed by whole-plasmid PCR using high-fidelity Pfu polymerase ...the I ORF was eliminated by a T-to-C ... See full document

... Upon virus infection, the IFN induction pathway is triggered by dsRNA molecules (intermedi- ates of viral replication) that can be sensed by cellular retinoic acid-inducible gene I product ... See full document

... EBV LF2, a gene deleted from the B95-8 reference strain (59, 61), encodes an Rta binding protein ...(6). LF2 was also identified as an IRF7 antagonist in a screen for EBV-encoded inhibitors of ... See full document

... during type I and II EBV latency to mediate viral DNA replication (25, ...through interferon- dependent and -independent pathways and present direct ev- idence that IRF-1 and IRF-2 activate ... See full document

... by Epstein-Barr virus (EBV) generates several types of latency with different profiles of gene expression but with expression of Epstein-Barr nuclear antigen 1 (EBNA-1) in ...in ... See full document

... Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1) is the principal oncogenic protein in the EBV transformation ...of interferon regulatory factor 7 (IRF-7) and activates IRF-7 protein ... See full document

... (wild type or S339A), which contains aa 57 to 427 of IRF3, and the Gal4- luciferase reporter system (60) were used to determine whether BGLF4 adapts a Pin1-mediated mechanism to degrade ... See full document

... during type I latency has received intensive ...an interferon-stimulated response element (ISRE) which lies up- stream of the Qp transcriptional start site and is bound by members of the ... See full document

... Cell Signaling) (data not shown). Comparable activation of PKR was observed in three other EBV-positive BL lines ana- lyzed (Daudi, KemI, and MutuI), as shown in Fig. 4, each of which expresses EBER-1 and EBER-2 at ... See full document

... To determine if the association of RecQL1 with Zta and OriLyt was functionally relevant for lytic replication, we gen- erated two different shRNAs specific for RecQL1 (Fig. 7). The shRNAs were expressed from lentivirus ... See full document

... pstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus as- sociated with a wide variety of neoplasms, including Burkitt’s lymphoma (BL), nasopharyngeal carcinoma, posttransplant lym- phoproliferative ... See full document

... EBV transforms adult primary B cells into continually grow- ing lymphoblastoid cell lines (LCLs) and concomitantly estab- lishes type III latency in vitro. LMP-1 is required for the trans- formation process: the ... See full document

... FIG 9 IFI16 recognition of the EBV genome is the sensor trigger for inflammasome induction in EBV latently infected LCL and Raji cells. (A and B) EBV latency (I, II, and III)-associated EBNA1 is not involved in ... See full document

... Adsorption of FITC-EBV and unlabeled EBV with SV-treated cells EBNA induction" in BJA-B cells by residual EBV' % EBNA positive Residual FITC-EBVa binding EBV adsorption with: to Raji cel[r] ... See full document

... The discoveries that integration sites occasionally co-localize with regions containing cancer associated genes JAK2 , PD-L1 and PD-L2 in gastric carcinomas and BACH2 , REL [r] ... See full document

... Because LF2 induces Rta SUMOylation, its repressive effects may extend beyond inhibition of DNA ...Interestingly, LF2 coexpression strongly repressed Gal4-Rta activation ...of LF2 on the Gal4 DBD ... See full document

... However, for the following reasons we presume they represent proteolytic degradation products generated in vivo: i immunoprecipitates of COS cells electroporated with pSV2CAT a plasmid s[r] ... See full document

... the Epstein-Barr virus (EBV) genome in the BL cell line Akata led to loss of tumori- genicity in SCID mice, suggesting an important oncogenic activity of EBV in B ... See full document

... infection. Epstein-Barr vi- rus (EBV), one of eight human herpesviruses, undergoes a primary, productive infection in epithelial tissues, followed by the establishment of latent infection in B lymphocytes ... See full document