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[PDF] Top 20 Evaluation of solubilized herpes simplex virus membrane antigen by enzyme linked immunosorbent assay

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Evaluation of solubilized herpes simplex virus membrane antigen by enzyme linked immunosorbent assay

Evaluation of solubilized herpes simplex virus membrane antigen by enzyme linked immunosorbent assay

... Two panels of 50 and 94 blood donor serum samples were used for evaluation of the ELISA, as were paired serum samples from 37 patients with diseases compatible with primary type 1 or 2 i[r] ... See full document

7

Evaluation of a Novel Aspergillus Antigen Enzyme Linked Immunosorbent Assay

Evaluation of a Novel Aspergillus Antigen Enzyme Linked Immunosorbent Assay

... Aspergillus antigen ELISA, we identified 45 and four cases that meet the EORTC/MSG criteria of proven and probable IA, ...GP assay, respectively (Table ...of antigen degradation during storage ... See full document

10

Evaluation of a commercially available enzyme linked immunosorbent assay for Giardia lamblia antigen in stool

Evaluation of a commercially available enzyme linked immunosorbent assay for Giardia lamblia antigen in stool

... d The because two children had specimens that were ELISA positive but microscopically negative on initial sensitivity of microscopy did not reach examination; on repeat examination of th[r] ... See full document

6

Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

... BC134 antigen are thus necessary and should use a large number of horse sera in order to ascertain which region of the BC134 antigen is specific to the serological diagnosis of ... See full document

5

Evaluation of a Commercial Glycoprotein Enzyme Linked Immunosorbent Assay for Measuring Vaccine Immunity to Varicella

Evaluation of a Commercial Glycoprotein Enzyme Linked Immunosorbent Assay for Measuring Vaccine Immunity to Varicella

... Fig. 1. Microphotograph images of VZV infected MGLu cell in FAMA test. The images were taken by Axioscope fluorescent microscope (A-F) and Leica SP2 confocal microscope (G and H). Reading criteria for FAMA using WHO ... See full document

8

Comparison of enzyme linked immunosorbent assay with enzyme linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus

Comparison of enzyme linked immunosorbent assay with enzyme linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus

... The enzyme-linked immunosorbent assay ELISA was compared with the enzyme-linked fluorescence assay ELFA for the detection of rubella antibody and herpes simplex virus antigen.. Test para[r] ... See full document

5

Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin streptavidin enzyme linked immunosorbent assay

Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin streptavidin enzyme linked immunosorbent assay

... The presence of HSV antigen in clinical specimens and stock virus preparations was confirmed by blocking the detection with rabbit and human HSV-1- and -2-specific antisera.. Preimmune s[r] ... See full document

6

Biotin avidin amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens

Biotin avidin amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens

... A biotin-avidin-amplified enzyme-linked immunosorbent assay B-A ELISA has been developed to detect herpes simplex virus type 1 HSV-1 and HSV-2 antigens in clinical specimens.. The test w[r] ... See full document

6

Rapid diagnosis of herpes simplex virus infections by enzyme linked immunosorbent assay

Rapid diagnosis of herpes simplex virus infections by enzyme linked immunosorbent assay

... A total of 457 clinical specimens were tested for the presence of herpes simplex virus HSV by isolation in cell culture and by enzyme-linked immunosorbent assay.. HSV antigen was detecte[r] ... See full document

5

Determination of herpes simplex virus type specific antibodies by enzyme linked immunosorbent assay

Determination of herpes simplex virus type specific antibodies by enzyme linked immunosorbent assay

... We determined type-specific antibodies to herpes simplex virus type 1 HSV-1 and type 2 HSV-2 by an indirect enzyme-linked immunosorbent assay, using as antigens HSV-1 glycoprotein gC-1 a[r] ... See full document

5

Herpes simplex virus type selective enzyme linked immunosorbent assay with Helix pomatia lectin purified antigens

Herpes simplex virus type selective enzyme linked immunosorbent assay with Helix pomatia lectin purified antigens

... Helix pomatia lectin-purified antigens with specific reactivity to herpes simplex virus type 1 HSV-1 and HSV-2 antibodies in human sera were used in an enzyme-linked immunosorbent assay.[r] ... See full document

5

Use of a New Dual Antigen Enzyme Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

Use of a New Dual Antigen Enzyme Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

... All 95 HIV-1 samples analyzed with ELISA-HIV2 gave neg- ative results with rpC2-C3. Thirty-one (32.6%) samples cross- reacted with rgp36, but the reactivity was significantly weaker than that of HIV-2 samples (mean S/CO ... See full document

5

Laboratory Diagnosis of Dengue Infection: Current Techniques and Future Strategies

Laboratory Diagnosis of Dengue Infection: Current Techniques and Future Strategies

... Dengue Virus, a member of the Flaviviridae ...the virus can cause the full spectrum of disease from asymptomatic infection to life-threatening ...of virus in specimens collected from clinically ... See full document

8

Low Sensitivity of NS1 Protein Tests Evidenced during a Dengue Type 2 Virus Outbreak in Santos, Brazil, in 2010

Low Sensitivity of NS1 Protein Tests Evidenced during a Dengue Type 2 Virus Outbreak in Santos, Brazil, in 2010

... NS1 tests are fast and easy to perform, becoming a valuable tool for the diagnosis of dengue acute infection. However, our data indicate that current NS1 assays should not be used in the Brazil- ian population before ... See full document

5

Comparison of enzyme linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens

Comparison of enzyme linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens

... plasmids. The wild type virus was cultured at the Kenya Medical Research Institute for more than 5 passages. RNAs from RVFV infected culture fluid of Vero cells were extracted by using the QIAamp viral mini kit ... See full document

8

Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme linked immunosorbent assay

Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme linked immunosorbent assay

... Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus SIV p27 core protein were developed for the isol[r] ... See full document

6

Usefulness of dengue  ns1 antigen as an early marker of dengue virus infection in a tertiary care hospital

Usefulness of dengue ns1 antigen as an early marker of dengue virus infection in a tertiary care hospital

... Out of the 184 patients who were positive for either NS1 Ag or IgM Ab or both, 49.46% belonged to the age group 21 – 40 years and 23.37% to the age group ≤10 years. The results of our study are similar to that of a study ... See full document

6

Detection of Herpes Simplex Virus Type 2 Specific Immunoglobulin G Antibodies in African Sera by Using Recombinant gG2, Western Blotting, and gG2 Inhibition

Detection of Herpes Simplex Virus Type 2 Specific Immunoglobulin G Antibodies in African Sera by Using Recombinant gG2, Western Blotting, and gG2 Inhibition

... cordant samples from the Uganda and Kenya panels. Since the rgG2 ELISA uses a purified baculovirus-derived recombinant protein, the use of native HSV antigens to inhibit the binding of gG2 antibodies to the recombinant ... See full document

6

Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus

Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus

... CHIKV assay, suggesting it may detect low levels of the antibodies ...Mayaro virus and other alphaviruses analyzed by the CHIKV immunosorbend assay, suggesting it is a CHIKV-specific ...the ... See full document

7

Sensitive Enzyme Immunoassay for Hepatitis B Virus Core Related Antigens and Their Correlation to Virus Load

Sensitive Enzyme Immunoassay for Hepatitis B Virus Core Related Antigens and Their Correlation to Virus Load

... inexpensive assay for determining HBV virus load, we targeted HBcrAg, which is comprised of HBcAg and HBeAg, products of precore/core gene and under the control of the same ...specific enzyme ... See full document

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