[PDF] Top 20 Evaluation of the Xpert® MTB/RIF assay and microscopy for the diagnosis of Mycobacterium tuberculosis in Namibia

... that MTB infection is distributed throughout Namibia, with males slightly more infected than ...of MTB cases, whereas Zambezi and Omaheke had the lowest numbers of MTB cases ...over ... See full document

... and Mycobacterium tuberculosis infections can be very high (4, ...7). Microscopy has a low sensitivity for the diagnosis of EPTB, and reliance on culture-based detection methods can lead to ... See full document

... The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in ... See full document

... and Xpert MTB/RIF ...nary tuberculosis 6 months prior to presenting with seizures and an enlarging brain ...clinical diagnosis was a brain tuberculoma, which was treated and ... See full document

... study: Tuberculosis is deadliest communicable disease which infects populations globally and it is most common cause of pleural ...Gene Xpert among suspected tuberculous pleural effusion ...smear ... See full document

... nary tuberculosis, but its diagnosis remains ...cell-free Mycobacterium tuberculosis DNA in pleural ef- fusion and an evaluation of a newly developed molecular assay for the ... See full document

... The Xpert MTB/RIF assay (Cepheid, Sunnyvale, CA) (1, 3–5, 9, 12, 13, 15, 19, 25) for the diagnosis of Mycobacterium tuberculosis has recently been endorsed by the WHO ... See full document

... the Xpert MTB/RIF test was unable to iden- tify nearly 21% of the culture positive cases, the median time to TB detection was significantly shorter than that for LJ and MGIT culture ...The ... See full document

... Sputum sample collection and processing. Participants provided sputum samples at time of enrollment (pretreatment) and at weeks 2, 4, 6, 8, and 12 of anti-TB therapy. Per protocol, a single specimen was obtained at all ... See full document

... accurate diagnosis of tuberculosis (TB) and fast de- tection of drug resistance are essential to ensure early initia- tion of appropriate antituberculotic treatment, adequately man- age the disease, and ... See full document

... the assay to detect 16 CFU/ml, in contrast to the 114-CFU/ml limit of detection of Xpert MTB/RIF ...the assay, such as a larger PCR chamber in the cartridge, appear to have improved its ... See full document

... making diagnosis by the conventional method of smear microscopy less ...ties, diagnosis of extrapulmonary TB is often made on the grounds of clinical suspicion alone, and many people receive the ... See full document

... probe assay (LPA) and Xpert MTB/RIF have been endorsed by the World Health Organization for the rapid diagnosis of drug-resistant ...the Xpert MTB/RIF on samples ... See full document

... smear microscopy, culture, Xpert and MTBDRplus ...in Xpert assay among the Beijing and non-Beijing MDR-TB isolates, we included only the RR isolates detected by Xpert ... See full document

... using Xpert MTB/RIF ...accurate diagnosis of RIF resistance in MTB strains ...of RIF-resistant MTB strains in specimens collected from TB presumptive patients in ... See full document

... Mycobacterium tuberculosis remains one of the most significant causes of death from an infectious ...of tuberculosis (TB), accounting for one-forth (24%) of the global TB ...resistance, Xpert ... See full document

... of tuberculosis (TB) cases in India are estimated to have extrapulmonary disease, and due to a lack of diagnostic means, they often remain ...of Mycobacterium tuberculosis and multidrug resistance is ... See full document

... smear microscopy (either Ziehl-Neelsen or auramine O staining) and solid (Löwenstein Jensen media) and liquid (mycobacterial growth indicator tubes [MGIT]; Becton, Dickinson, Sparks, MD) cultures of each sample, ... See full document

... TB: Xpert MTB/RIF assay, Cepheid, USA; TB: tuberculosis; MTB: Mycobac- terium tuberculosis; KJ: Kenneth-Jones; GA: gastric aspirates; Spm: sputum; TRS: treatment response ... See full document

... pairing Xpert with liquid culture (MGIT where available or MODS as an al- ...addition, Xpert MTB/RIF is able to rapidly detect RIF ...with RIF resistant strains and no robust ... See full document