[PDF] Top 20 Expression and characterization of duck enteritis virus gI gene

... The gI gene is located in unique short region (Us) within the herpesviral genome, its homolog almost existed in all ...The gI gene encoding membrane protein glycoprotein I(gI) is ... See full document

... For immunofluorescence assays, monolayers of duck embryo fibroblast (DEF) cells were infected (MOI 5) with the DEV strain Chv and then incubated for 30 h at 37°C. Cells were fixed with 4% paraformaldehyde for 30 ... See full document

... for expression the UL31 ...optimize expression, the bacterial culture was grown at 37°C until the optical density at 595 nm was ...tein expression was induced by the addition of ... See full document

... gE gene in DPV-infected cells The total RNA isolated from mock-infected and DPV- infected cells was verified by ...gE gene was analyzed by real-time quantitative PCR with SYBR Green I and reverse ... See full document

... UL16 gene of DEV was composed of 1089 nucleotides, which encoded 362 amino ...UL16 gene was highly conserved in herpesvirus family. The UL16 gene was cloned into a pET prokaryotic expression ... See full document

... To study the expression kinetics of UL53 gene, DEF were either mock or infected with DEV as described above and harvested at 10, 14, 24, 36, and 48 h.p.i. Cell lysates were in SDS sample buffer, which were ... See full document

... for expression in prokaryo- tic ...for characterization of pUL55 by Western blotting assay and indrect ...the expression of this gene appeared at the late stage of infection in infected DEFs ... See full document

... Methods: Duck enteritis virus US10 was amplified and cloned into the recombinant vector ...the expression level and subcellular localization of US10 in infected cells at different time ... See full document

... gC gene from the genomic DNA was amplified and cloned into a T/A cloning vector pMD18-T, gen- erating a recombinant cloning plasmid pMD18-T/gC (Figure ...gC gene fragment, which was obtained by digestion of ... See full document

... Intracellular viral protein localization is associated with viral protein function, but intracellular DEV pUL41 localization is undocumented. We analyzed DEV pUL41 intracellular localization by IFA using the mouse anti- ... See full document

... the expression of miR-30a-5p was downreg- ulated and that Beclin-1 was upregulated after CHv in- fection of DEF ...Beclin-1 gene is recognized as a critical regulatory gene during autophagosome ... See full document

... HA expression cassette con- taining a pMCMV IE promoter and a consensus HA gene (GenBank: KP019932) was synthesized and cloned into T- Vector pMD19 (Simple; Takara, Otsu, Japan) with slight modification to ... See full document

... Results: DEV UL15 consists of two exons with a 3.5 kilobases (kb) inron and transcribes into two transcripts: the full-length UL15 and an N-terminally truncated UL15.5. The 2.9 kb UL15 transcript encodes a protein of 739 ... See full document

... curve of Ct(threshold cycle) against initial target copy number [30]. In our reaserch, the plasmids pMD18-T/ UL55 and pMD18-T/b-actin were particularly con- structed to generate standard curves. The copy numbers of ... See full document

... disease. Because glycoproteins are the major antigens recognized by the infected host ’ s immune system and play an important role in mediating target cell infection, cellular entry of free viruses, and the maturation or ... See full document

... sequencing, gene and protein expression analysis, protein structure and interaction analysis, and pathway or network analysis ...the expression and prepare the polyclonal antibody against the ... See full document

... Duck enteritis virus (DEV) is a natural pathogen of ducks and causes duck viral enteritis, an acute, conta- gious, and lethal disease affecting waterfowl belonging to the family ... See full document

... Results: The DEV UL51 gene product was identified as an approximate 34 kDa protein in DEV- infected cells analyzed by western blotting. Computer aided analysis suggested that DEV pUL51 is not targeted to the ... See full document

... The DEV UL54 protein showed nuclear localization The intracellular distribution of DEV UL54 protein was confirmed through IFA using rabbit anti-UL54 serum. As shown in Fig. 5, UL54 protein-specific fluorescence was ... See full document

... (EGFP) expression was observed in cells transfected with pEGFP and pVP1-EGFP but not with pVP2-EGFP ...VP2 expression in the eukaryotic expression system ...diminished expression of the VP2 ... See full document