[PDF] Top 20 Expression and characterization of the UL31 protein from duck enteritis virus

... the UL31 gene was amplified from the genome of DEV and successfully expressed in a prokaryotic expression ...the UL31 gene product of DEV. We found that the UL31 gene was transcribed ... See full document

... this protein did not contain a nuclear localiza- tion sign ...362-aa protein. This provided further evidence that the deduced protein is UL16 ...UL16 protein was conserved throughout the ... See full document

... Preimmune serum was collected prior to immunization. New Zealand white rabbits were immunized firstly intra- dermally with a mixture of 1 mg purified recombinant rVP19c or rVP19c(N) mixed with an equal volume of complete ... See full document

... Different intracellular localizations may reflect different functions of envelope proteins, e.g., it has been reported that, HSV gE/gI accumulated in the trans-Golgi network (TGN) at early times and then redistributed to ... See full document

... the expression kinetics of UL53 gene, DEF were either mock or infected with DEV as described above and harvested at 10, 14, 24, 36, and 48 ...specific protein binding was blocked through dealing with PVDF ... See full document

... recombinant protein Recombinant plasmids containing the encoding region of DEV UL55 were constructed for ...KDa protein and it was mainly in the insoluble fraction(Figure 2, Lane ...optimal ... See full document

... and expression kinetics at different time points post-infection using RT-qPCR and western blot analysis, ...a protein synthesis inhibitor, while transcription of E and L genes is suppressed in the presence ... See full document

... US10 protein in a prokaryotic expression system to generate antiserum that recognizes US10 to better investigate its expression levels and subcellular localization in DEV-infected ...US10 ... See full document

... To confirm the intracellular distribution of pUL38, DEF cells were plated on coverslips and infected with DEV at an MOI of 5. The cells were processed at 8 h, 18 h, 30 h, and 56 h.p.i., and pUL38 was detected using ... See full document

... Microtubule-associated protein light chain (LC3) is an autophagy marker protein on the membranes of ...LC3II expression measures the number of autophagosomes [22]. The expression level of ... See full document

... its expression profile provides vital information on what it is doing at any given moment ...Gene expression levels change over time, as proteins inter- fere with gene ...gene expression control, in ... See full document

... immunoreactive protein was visualized by using diamino benzidine (DAB). From the result, we can see the purified tgK, which was recognized by rabbit anti-tgK polyclonal antibody, was apparent on ... See full document

... envelops from the inner lamella of nuclear membrane, which would fuse with the membrane of the endoplasmic ...homologous protein of HSV-1, VZV-1, and PRV [32-34], and suggested that DPV gE pro- tein might ... See full document

... the expression of the full-length gK gene (fgK) in a prokaryotic system is difficult because of the presence of suboptimal exon and transmembrane domains at the ...prokaryotic expression of the gK gene by ... See full document

... a protein of 739 amino acids with an approximate molecular mass of 82 kiloDaltons (kDa), whereas the ...its expression was sensitive to cycloheximide and phosphonoacetic ... See full document

... amplified from the DEV genome and cloned into the pPAL7 vector, generating the recom- binant plasmid pPAL-UL54, which was transformed into ...subsequent protein expression [30, ...and ... See full document

... To identify the precise localization of DEV pUL51 in DEV- infected cells, TIEM was carried out. Immunoelectron microscopy showed that at 6 h p.i., only a little pUL51- specific immuno-labeling was first observed in the ... See full document

... to virus infection, replica- tion and antiviral ...UL55 protein (pUL55) are encoded only among alpha herpes viruses ...simplex virus 2 (HSV-2) UL55 protein revealed that the product of HSV-2 ... See full document

... extracted from purified virus as described previ- ously (24) and was used to construct the DEV fosmid library according to the manufacturer’s instructions for the CopyControl fosmid library production kit ... See full document

... DHBcAg polypeptide exhibits heterogeneity in SDS-PAGE that can be completely removed following treatment of native core particles with alkaline phosphatase Fig.. 3, suggesting that the p[r] ... See full document