[PDF] Top 20 Folding and Dimerization of Tick-Borne Encephalitis Virus Envelope Proteins prM and E in the Endoplasmic Reticulum
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Folding and Dimerization of Tick-Borne Encephalitis Virus Envelope Proteins prM and E in the Endoplasmic Reticulum
... major virus-derived bands could be detected in the reduced samples, i.e., prM, E, and NS1, all of which migrated with the expected mobilities (25, 52, and 45 kDa, ...the E and NS1 bands was ... See full document
12
Resuscitating Mutations in a Furin Cleavage-Deficient Mutant of the Flavivirus Tick-Borne Encephalitis Virus
... mutant prM( ⌬ R88) in this system, three litters of suckling mice were inoculated intracranially with aliquots of supernatant de- rived from BHK-21 cells transfected with prM( ⌬ R88) ...wild-type ... See full document
11
Proteolytic activation of tick-borne encephalitis virus by furin.
... two envelope proteins, E and ...cell, prM is cleaved by a cellular enzyme, and this processing step can be blocked by treatment with agents that raise the pH of exocytic ...with ... See full document
7
Intracellular Assembly and Secretion of Recombinant Subviral Particles from Tick-Borne Encephalitis Virus
... with E in the ER of cells, we were not able to detect any interaction between the TBE virus prM and E and calnexin or calreticulin by immunoprecipita- ...the E protein from Japanese ... See full document
13
Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form.
... expressing prM and E proteins from Japanese encephalitis virus, and to particles derived from other mosquito-borne flaviviruses which have since been produced by using ... See full document
5
Japanese Encephalitis Virus Infection Initiates Endoplasmic Reticulum Stress and an Unfolded Protein Response
... for virus replication (5, 47, 55, ...ER-resident proteins and lipid components for the ...the virus-induced ...viral proteins are gly- cosylated and accumulated in the ER lumen, namely, the ... See full document
10
Mapping of Functional Elements in the Stem-Anchor Region of Tick-Borne Encephalitis Virus Envelope Protein E
... protein E stem-anchor region, we engineered a series of C-terminal deletions into wild-type ex- pression plasmid SV-PEwt (1), which has been used previously for correct coexpression of prM and E ... See full document
8
Recombinant subviral particles from tick-borne encephalitis virus are fusogenic and provide a model system for studying flavivirus envelope glycoprotein functions.
... of prM in ...uncleaved prM proteins (2, 16). To see whether the presence of prM in RSPs would have a similar inhibitory effect, prM-containing RSPs obtained from ammonium ... See full document
9
Identification of linear human B cell epitopes of tick borne encephalitis virus
... The envelope protein E is a class II viral fusion ...other envelope protein, prM, is cleaved by furin during viral maturation and the pr moiety is released as a result of conformational ... See full document
9
Viperin Restricts Zika Virus and Tick-Borne Encephalitis Virus Replication by Targeting NS3 for Proteasomal Degradation
... and virus particle formation (20, ...TBEV E, prM, NS2A, and NS2B were expressed in HEK293T cells along with hemagglutinin (HA)-tagged or nontagged NS3 and/or ...viral proteins (prM, ... See full document
14
Oligomeric rearrangement of tick-borne encephalitis virus envelope proteins induced by an acidic pH.
... the envelope protein E, mature flaviviruses contain a capsid protein, C (15 kDa), which is associated with the positive-stranded RNA genome in an isometric nucleocap- sid structure, and a second membrane ... See full document
6
The Transmembrane Domains of the prM and E Proteins of Yellow Fever Virus Are Endoplasmic Reticulum Localization Signals
... of prM and E in the absence of other viral components suggests that lateral interactions between these envelope proteins are a major driving force leading to particle assembly ...nonstructural ... See full document
12
Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum
... limited folding rate in the ER of ...plex proteins, such as human ...of folding factors in their maturing pro- cess ...and folding enzymes, like protein disulfide isomerases (PDIs) and ... See full document
18
Differences in the Postfusion Conformations of Full-Length and Truncated Class II Fusion Protein E of Tick-Borne Encephalitis Virus
... the E protein. For this purpose preparations of E trimers and sE trimers were incubated for 10 min at room temperature, 50, 55, 60, 65, and 70°C, and then their oligo- meric state was analyzed by rate zonal ... See full document
5
The expression of the chemokine receptor CCR5 in tick-borne encephalitis
... viral encephalitis, the role of CXCR3 and CCR5 has been stud- ied mainly in animal models and shows differences de- pending on virus type and strain virulence [15, 20, ...gitis virus [23] and ... See full document
17
Variation of the Specificity of the Human Antibody Responses after Tick-Borne Encephalitis Virus Infection and Vaccination
... TBE virus infection and vaccination, and depletion of DIII-spe- cific antibodies from the serum pools had no measurable effect on virion binding and neutralization activities ...of E dimer-specific epitopes ... See full document
13
Role of Metastability and Acidic pH in Membrane Fusion by Tick-Borne Encephalitis Virus
... Conversion of dimers to trimers. Although heat treatment did not result in functional activity, we were interested in finding out whether heat treatment can induce the character- istic dimer-trimer transition normally ... See full document
7
IDENTIFICATION OF THE SIBERIAN TYPE TICK-BORNE ENCEPHALITIS VIRUS AND SEROLOGICAL SURVEILLANCE IN MONGOLIA
... of tick-borne encephalitis virus in Mongolia was performed by Russian scientist ...the virus was first isolated from the liver of the marmot hunted in Dornod ...against ... See full document
8
Association of poliovirus proteins with the endoplasmic reticulum.
... Even though proteins such as P3-4a, P2-Sb, P2-X, and P3-9 are more tenaciously associated with cellular membranes than other virus-specific proteins, only P3-7c was completely released d[r] ... See full document
7
Molecular Basis of the Divergent Immunogenicity of Two Pediatric Tick-Borne Encephalitis Virus Vaccines
... Nd virus. Virus neutralization titers were cal- culated according to the statistical approximation method of Spearman- ...with virus neutralization titers of ⱖ ...Neudörfl E protein was done ... See full document
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