[PDF] Top 20 Function of Rta Is Essential for Lytic Replication of Murine Gammaherpesvirus 68

... the Rta proteins of Epstein Barr virus (EBV), Kaposi’s sarcoma-associated herpesvirus (KSHV, or HHV-8), and murine gammaherpesvirus 68 (MHV-68; also referred to as ␥ HV68) play an ... See full document

... 2 RTA activates the ORF48 promoter in the reporter ...48pRRE function. The fold activation by RTA for the vector is significantly different from that for each of the ORF48 promoter reporter plasmids ... See full document

... The replication and transcription activator (RTA), mainly encoded by open reading frame 50, is an imme- diate-early gene product that is conserved among all characterized ...that RTA proteins of ... See full document

... viral lytic replication in which newly synthesized ORF45 may function under BAC transfection con- ditions, we first attempted to identify a near-single-round viral replication time point ... See full document

... the replication of cer- tain viruses, including HIV-1 and Ebola ...and murine gammaherpesvirus 68 (MHV-68), whose life cycle has latent and lytic ...the lytic phase and is ... See full document

... MHV-68 Rta alone was sufficient to reactivate the virus in B ...an Rta expression plasmid induced the expression of early and late viral RNAs and proteins, activated lytic replication ... See full document

... that RTA expression is sufficient to induce the ubiquitina- tion and degradation of these transcriptional regulators, it re- mains an open question whether RTA possesses intrinsic E3 ligase activity toward ... See full document

... of lytic replication and reactivation from ...50-encoded Rta is the only immediate-early gene product that appears to be conserved among all characterized ...and gammaherpesvirus 68 ( ␥ ... See full document

... All mammalian and avian herpesviruses encode conserved glycoproteins—glycoprotein B (gB), gH, gL, gM, and gN—that mediate the assembly of infectious virions, their exit from the cell, and their subsequent fusion with new ... See full document

... of Rta in ...EBV replication was previously assessed using a cotransfection replication assay employing a plasmid containing oriLyt and expression vectors for replication proteins (7, 12, 13, ... See full document

... A disruption of ORF45 blocked viral immediate-early and early gene expression, suggesting that ORF45 plays an essen- tial role in viral infection immediately after the viral genome enters the nucleus. It seemed that the ... See full document

... to function in transcriptional activation of the adjacent viral ...the lytic rep- lication ...a replication-incompe- tent virus, although replication was attenuated ... See full document

... a function of time postinfection in C57BL/6 and B-cell-deficient mice reveals that B cells are important for controlling the frequency of cells reactivating latent g ...detected lytic gHV68 ... See full document

... Murine gammaherpesvirus 68 (MHV-68), Kaposi’s sarcoma-associated herpesvirus (HHV-8), and Epstein- Barr virus (EBV) are all members of the gammaherpesvirus family, characterized by ... See full document

... MHV68 lytic replication, the precise mecha- nisms by which this is accomplished remains to be ...viral replication (58, 74, ...during lytic replication are not known, we speculate that ... See full document

... enhancers, function to remodel chromatin structure, and mediate communication between sequence-spe- cific transcriptional factors and the general transcription ma- ...during lytic infection of HSV-1, ... See full document

... virus replication, BHK-21 cells were infected with MHV-68 at an MOI of 5 in the presence or absence of cyclohexi- mide (CHX; a protein synthesis inhibitor) (400 ␮g/ml) or phos- phonoacetic acid (PAA; a ... See full document

... the function of several MHV-68-specific genes has been elucidated, the function of M10a-c has not been investi- gated so ...during lytic and latent infections of mice with MHV-68 by ... See full document

... The lytic cycle functions of gammaherpesviruses have received relatively little attention to date, at least in part due to the lack of a convenient experimental ...The murine gammaherpesvirus ... See full document

... and murine gammaherpesvirus 68 ...viral replication, including interactions with cellular and viral proteins, are not ...for lytic viral replication consisting of 15 viral ... See full document