[PDF] Top 20 Functional Analysis of PA Binding by Influenza A Virus PB1: Effects on Polymerase Activity and Viral Infectivity
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Functional Analysis of PA Binding by Influenza A Virus PB1: Effects on Polymerase Activity and Viral Infectivity
... the PA-binding site of PB1. PB1 and PA interact to assemble influenza virus transcription and replica- tion complexes, which also include ...of PB1 was shown ... See full document
10
Low Polymerase Activity Attributed to PA Drives the Acquisition of the PB2 E627K Mutation of H7N9 Avian Influenza Virus in Mammals
... H7N9 PA determines the sensitivity of PB2 627E polymerase to variations in human ANP32A ...in viral genome transcription and replication of influenza virus ...avian-like viral ... See full document
16
DnaJA1/Hsp40 Is Co-Opted by Influenza A Virus To Enhance Its Viral RNA Polymerase Activity
... enhances viral RNA synthesis in an RNP reconstitu- tion ...and PA subunits specifically, it can be deduced that DnaJA1 may play a role in viral RNA synthesis ...the effects of DnaJA1 on ... See full document
12
A 48-amino-acid region of influenza A virus PB1 protein is sufficient for complex formation with PA.
... the polymerase complex, being responsible for RNA chain ...putative functional domains have been identified in PB1. Influenza A virus PB1 expressed in the absence of other ... See full document
8
Involvement of Influenza Virus PA Subunit in Assembly of Functional RNA Polymerase Complexes
... model viral genome containing a reporter gene (26, 42, ...ts53 PA caused temperature-sensitive expression (data not ...wild-type PA and ts53 PA, ts53 PA did not efficiently inhibit the ... See full document
Functional Genomics Reveals Linkers Critical for Influenza Virus Polymerase
... the PB1, PB2, and PA gene segments of influenza A/WSN/1933 H1N1 (WSN) using a Mu-transposon mutagenesis method previously described ...of influenza virus to transfect mutant plasmid ... See full document
10
Mutational Analysis of the Binding Pockets of the Diketo Acid Inhibitor L-742,001 in the Influenza Virus PA Endonuclease
... of PA allowed us to estimate the impact of the PA mutations on the overall polymerase activity of the vRNP com- ...the activity of the reconstituted vRNPs (G81K, G81D and G81V, I120K, ... See full document
15
Mutational Analyses of Packaging Signals in Influenza Virus PA, PB1, and PB2 Genomic RNA Segments
... various effects on reporter vRNA packaging ...the PA protein and hence of the viral polymerase (which mediates vRNA replication and transcription), we tested the abilities of these PA ... See full document
8
Characterization of Influenza Virus PB1 Protein Binding to Viral RNA: Two Separate Regions of the Protein Contribute to the Interaction Domain
... PB2 binding domain and the C-terminal RNA-binding sequences ...the PA or PB2 subunits could modify the vRNA-binding activity of PB1 protein in the poly- merase complex remains to ... See full document
7
Reassortment and Mutation of the Avian Influenza Virus Polymerase PA Subunit Overcome Species Barriers
... Polymerase activity assays were performed with human 293T cells expressing a luciferase-based viral reporter construct, the vi- ral nucleoprotein (NP), and the polymerase subunits PB1, ... See full document
8
Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit.
... vaccinia virus encoding T7 RNA ...biological activity of these mutants was tested by determining their capacity to (i) reconstitute RNA polymerase activity in vivo by cotransfection with ... See full document
9
The Replication Activity of Influenza Virus Polymerase Is Linked to the Capacity of the PA Subunit To Induce Proteolysis
... nonproteolytic PA proteins are able to transcribe but unable to ...CAT activity obtained by reconstitution of viral polymerase with nonproteolytic PA mutants could be the con- sequence ... See full document
6
PA Residues in the 2009 H1N1 Pandemic Influenza Virus Enhance Avian Influenza Virus Polymerase Activity in Mammalian Cells
... pandemic influenza virus (pH1N1) is a swine-origin reassortant containing human, avian, and swine influenza ...the polymerase complex of the pH1N1 strain A/Cali- fornia/04/2009 (Cal) is highly ... See full document
9
The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA.
... endonuclease activity (29) in vitro, and mutations in the PB2 gene alter the usage of capped primers in transcription reactions in vitro ...either PB1 or PA proteins or may affect their inter- ...the ... See full document
5
Temperature-Sensitive Mutants in the Influenza A Virus RNA Polymerase: Alterations in the PA Linker Reduce Nuclear Targeting of the PB1-PA Dimer and Result in Viral Attenuation
... containing PA mutations. We assessed the effects of PA linker mutations on the replication/transcription activity of the viral polymerase, us- ing a minireplicon ...modified ... See full document
15
Mutational and Metal Binding Analysis of the Endonuclease Domain of the Influenza Virus Polymerase PA Subunit
... of PA-Nter in the pres- ence of 100 mM MgCl 2 and observed a single metal ion only in position M1, even in the presence of mononucleotide phos- phates ...low-affinity binding site for Mg 2⫹ lies outside the ... See full document
9
In Vitro Assembly of PB2 with a PB1-PA Dimer Supports a New Model of Assembly of Influenza A Virus Polymerase Subunits into a Functional Trimeric Complex
... for activity by the ApG-primed and globin-primed transcription assays in vitro, without the addition of further promoter RNAs ...No activity was obtained with the PB1-PA dimer assem- bled with ... See full document
6
Amino Acid Residues in the N-Terminal Region of the PA Subunit of Influenza A Virus RNA Polymerase Play a Critical Role in Protein Stability, Endonuclease Activity, Cap Binding, and Virion RNA Promoter Binding
... inhibited polymerase activity severely ...these PA mutants ...the functional assembly of the trimeric complex of PB1, PB2, and PA, a TAP-tagged PB2 polymerase complex was ... See full document
10
HDAC6 Restricts Influenza A Virus by Deacetylation of the RNA Polymerase PA Subunit
... IAV polymerase function (27); phosphorylation of IAV NS1 impairs its interferon- antagonistic activity (28), and phosphorylation of M1 is crucial for its nuclear import (29); the acetylation of IAV HA is ... See full document
13
Investigation of Influenza Virus Polymerase Activity in Pig Cells
... for influenza virus host range restriction and adaptation to a new species is not fully ...the viral glycoprotein hemagglutinin (HA) for differently linked sialic acid receptors (NeuAc␣2,3Gal or ... See full document
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