[PDF] Top 20 Functional Analysis of Cell Surface-Expressed Hepatitis C Virus E2 Glycoprotein
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Functional Analysis of Cell Surface-Expressed Hepatitis C Virus E2 Glycoprotein
... and E2 localize to the ER in infected cells (4, 6, ...flaviviruses, virus morphogenesis may involve budding into the ER and subse- quent transport of viral particles through the host cell secretory ... See full document
9
Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry
... alters E2 binding to SR-BI ...other cell lines coexpressing CD81 and SR-BI that are nonpermissive to HCVpp infection and which are of nonhepatic origin (5, ...molecule(s), expressed in hepatic cells ... See full document
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Definition of a Conserved Immunodominant Domain on Hepatitis C Virus E2 Glycoprotein by Neutralizing Human Monoclonal Antibodies
... to E2 and high neutralizing activity ( ⬎ 1: 10,000 titer) against genotype 1a ...Epstein-Barr virus and used to produce human hybridomas, as described previously ...recombinant E2 proteins ... See full document
6
Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay
... single C-terminal transmembrane (TM) domain and contain an N-terminal ectodomain (36) ...and E2 also encode the signal sequence of the downstream proteins (E2 and p7, respectively), and the signal ... See full document
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Hepatitis C Virus Envelope Glycoprotein E1 Forms Trimers at the Surface of the Virion
... E1 glycoprotein is detected as a trimer on HCV ...the surface of the viral par- ticle, showing some oligomeric changes during the assembly pro- cess ...imentation analysis in sucrose gradients under ... See full document
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Folding of Hepatitis C Virus E1 Glycoprotein in a Cell-Free System
... The hepatitis C virus (HCV) envelope proteins, E1 and E2, form noncovalent heterodimers and are leading candidate antigens for a vaccine against ...mammalian cell expression systems ... See full document
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A systematic analysis of a broadly neutralizing antibody AR3C epitopes on Hepatitis C virus E2 envelope glycoprotein and their cross-reactivity
... The neutralizing motif indicates that conservative repla- cements at positions 430 (N®Q), 431 (D®E) and 438 (L®I) will likely not affect binding affinities suffi- ciently to abolish neutralization. In addition, position ... See full document
9
Identification of Amino Acid Residues in CD81 Critical for Interaction with Hepatitis C Virus Envelope Glycoprotein E2
... in cell acti- vation, proliferation, motility, and metastasis, as well as in cell fusion ...widely expressed, its level of ex- pression varies in specific cell lineages and during differentia- ... See full document
8
Binding of Hepatitis C Virus E2 Glycoprotein to CD81 Does Not Correlate with Species Permissiveness to Infection
... Hepatitis C virus (HCV) is the major etiologic agent of non-A non-B hepatitis, infecting an estimated 400 million peo- ple worldwide ...chronic hepatitis, cirrhosis, and hepatocellular ... See full document
6
Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
... the cell surface by associating with other tetraspanins, lineage-specific proteins, integrins, major histo- compatibility complex molecules, and signaling proteins in a cell type-dependent manner ... See full document
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Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding
... Hepatitis C virus (HCV) cell entry involves interaction between the viral envelope glycoprotein E2 and the cell surface receptor ...conserved E2 determinants ... See full document
10
Hepatitis C Virus Envelope Glycoprotein E2 Glycans Modulate Entry, CD81 Binding, and Neutralization
... of E2 glycan re- moval on antibody ...important functional do- mains on ...of E2, indicating that this do- main is exposed on the virion ...soluble E2 and virions to target cells, suggesting ... See full document
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Conservation of the Conformation and Positive Charges of Hepatitis C Virus E2 Envelope Glycoprotein Hypervariable Region 1 Points to a Role in Cell Attachment
... an analysis of the largest possible number of nonredundant HVR1 sequences of natural HCV variants collected from the EMBL ...quasispecies analysis (see reference 35 for further ... See full document
8
Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells
... the E2 core structure (Fig. 5). This analysis highlighted the differential glycan sizes observed in the two expression systems, particularly those proximal to key epitopes such as N6 ...protein ... See full document
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A Retention Signal Necessary and Sufficient for Endoplasmic Reticulum Localization Maps to the Transmembrane Domain of Hepatitis C Virus Glycoprotein E2
... of E2 maps to its TMD. Replace- ment of the TMD of E2 with the anchor sequence of CD4 or a GPI moiety was sufficient for its export to the cell ...of E2 was retained in the ER. This indicates ... See full document
9
Identification and Characterization of Broadly Neutralizing Human Monoclonal Antibodies Directed against the E2 Envelope Glycoprotein of Hepatitis C Virus
... and E2. Additionally, any changes found in the NIH HCV database for E2 amino acids 413, 418, and 420, critical residues according to alanine scan- ning mutagenesis, were also engineered into the full-length ... See full document
10
Formation of native hepatitis C virus glycoprotein complexes.
... were expressed separately, MAb H2 was shown to recognize E2 but not E1 ...the E2 precursor, E2-NS2 ...and E2 by MAb H2 were very similar when these proteins were coexpressed ...is ... See full document
8
Mutations within a Conserved Region of the Hepatitis C Virus E2 Glycoprotein That Influence Virus-Receptor Interactions and Sensitivity to Neutralizing Antibodies
... mutant virus. The cell culture adaptive E2 mutations characterized above oc- curred in viral variants that emerged after prolonged passaging of infected cells under standard tissue culture ... See full document
14
Identification of Conserved Residues in Hepatitis C Virus Envelope Glycoprotein E2 That Modulate Virus Dependence on CD81 and SRB1 Entry Factors
... the surface of E2 protein ...on virus assembly also suggests that these res- idues are not involved in interactions with HCV glycoprotein E1, and the lack of effect on virus entry and ... See full document
14
Structural Flexibility of a Conserved Antigenic Region in Hepatitis C Virus Glycoprotein E2 Recognized by Broadly Neutralizing Antibodies
... SPR analysis of Fab binding to aa 412 to ...and expressed as the occupancy of binding sites. (A) Real-time SPR analysis of the interaction of the epitope peptides derived from genotype 1a strain ... See full document
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