[PDF] Top 20 Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay

... quent fusion events and perhaps larger ...E2 proteins that contained the ectodomains of the HCV E1 and E2 proteins and the signal sequences, transmembrane domains, and cytoplasmic tails of the VSV-G ... See full document

... the luciferase reporter gene in which we introduced separately the above-identified determinants of intergenotypic incompat- ibility. To further narrow down the determinants, we excluded some of the conserved fragments ... See full document

... E1E2 proteins of all mutants were expressed on the cell surface as effectively as were the WT proteins, we then determined whether mutations in the E1 fusion pep- tide-like domain affected the ... See full document

... E1 proteins in ...E2 envelope proteins in trans ...2a cell culture-infectious HCV (HCVcc) strain (JFH1 strain) was ...trans-complemented virus was designated HCVΔE1. Reverse ... See full document

... HCV cell culture systems, researchers used recombinant envelope proteins which were immobilized on the surface of a plate to investigate the effect of HCV envelope proteins on the NK ... See full document

... short peptides and translocated into the endoplasmic reticu- lum or loading onto HLA class I molecules (1, 39, 41). The HLA-peptide binding depends on amino acids known as an- chor residues (41). The complex of a peptide ... See full document

... 7. Virus-cell fusion ...BlaM-Vpr fusion protein were pelleted by ultracentrifugation through 25% ...cytometry analysis after 16 h of incubation. (B and C) The ability of the Env ... See full document

... viral envelope proteins and cell surface receptors and ...robust cell culture system to support the replication of various HCV genotypes, surrogate systems have been devel- oped to examine the ... See full document

... T/F virus was likely responsible for productive infection in ...T/F virus E1E2 amino acid sequence was found to be identical to that of a minor variant present among donor DA ...this analysis to ... See full document

... the virus interacts with its receptor represents an important factor in determining both the onset and the progression of viral infection, the first objective of this work was to determine the affinity of the ... See full document

... the hepatitis C virus (HCV) life ...the fusion step between viral and cellular mem- ...HCV cell-to-cell transmission is dependent on the vesicular pH. Using the selective ... See full document

... leukemia virus (MuLV), Mason-Pfizer monkey virus, and equine infectious anemia virus, the envelope TM protein cytoplasmic tail is further processed by the viral pro- tease after virus ... See full document

... HCV envelope proteins recover the ability to infect various mammalian cells, although they have not examined a pseudotype virus bearing both of the envelope proteins ...E2 ... See full document

... and envelope glycoproteins E1 and E2 are the structural components of the viral ...Non-structural proteins are involved in the viral life cycle ...host cell signal peptidases. These proteins ... See full document

... Construction of recombinant cDNA. A cDNA cassette allowing replacement of the ectodomain or transmembrane and cytoplasmic domains of influenza A virus HA was constructed. Unique restriction sites were introduced ... See full document

... mutational analysis of this region and found that the conserved tryptophans play a crucial role in the membrane fusion ...env-mediated fusion at a post lipid mixing stage (Kliger et ...second ... See full document

... Hepatitis C virus (HCV) RNA replication occurs in the cytosol of infected cells within a specialised membranous ...(NS) proteins are associated and organised within these structures remains ... See full document

... measles virus (MV) isolated in B95a cells could be adapted to Vero cells after several blind ...Vero cell-adapted (T11Ve-23) MV strain and identified amino acid substitutions R516G, E271K, D439E and G464W ... See full document

... the cell-cell binding experiments described above, the fluorescence measured at the temperatures at which fusion takes place could come both from bound fluorescent SupT1 cells and from SupT1 cells ... See full document

... Cells coinfected with a recombinant adenovirus which contained the large envelope gene and with either HS2.HP or HS.HP expressed similar quantities of the large, middle, and major envelo[r] ... See full document