[PDF] Top 20 Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics.
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Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics.
... 23, the pestivirus plO protein may be the functional homolog of HCV NS4A, because sequences in this region of the pestivirus polyprotein appear to be required for the serine proteinase-d[r] ... See full document
11
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation.
... of NS3 via NS4A might have a dual function: to increase the proteinase concentration close to the substrate and to sequester the proteinase from rapid cel- lular ...that cleavage at the ... See full document
10
Hepatitis C virus-encoded NS2-3 protease: cleavage-site mutagenesis and requirements for bimolecular cleavage.
... of hepatitis C virus (HCV) is thought to be mediated by a virus-encoded protease composed of the region of the polyprotein encoding NS2 and the N-terminal one-third of ...the NS3 ... See full document
10
Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease
... whereas processing at the NS5A/5B site occurs in its absence but is significantly increased by this cofactor (3, 13, 26, ...sites, processing at the NS3/4A site is an intramolecular reaction, as ... See full document
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Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease.
... other serine proteases belonging to the chymotrypsin family ...catalytic serine 1165 of the polyprotein was mutated to alanine, cleavage at the NS3/4A, NS4A/4B, NS4B/5A, and NS5A/5B sites was ... See full document
6
Probing the substrate specificity of hepatitis C virus NS3 serine protease by using synthetic peptides.
... steady-state kinetics were established by using substrate concentrations two to three orders of magni- tude higher than the HCV NS3/4A protease concentration and allowing less than 10% cleavage of ... See full document
6
Specificity of the hepatitis C virus NS3 serine protease: effects of substitutions at the 3/4A, 4A/4B, 4B/5A, and 5A/5B cleavage sites on polyprotein processing.
... Since mutations blocking cleavage at the 4A/4B site do not affect trans processing at other sites as discussed below, the degree of inhibition of cleavage at the 4A/4B site could be moni[r] ... See full document
9
NS3-4A of hepatitis C virus is a chymotrypsin-like protease.
... Polypeptide NS3 D C-4 D C was chosen as a protease source in protease inhibitor assays because of its high trans- cleavage ...activity. NS3 D N-4 D C was processed by ... See full document
6
A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro.
... A virus-encoded serine proteinase mediates four site-specific cleavages in the hepatitis C virus ...protein NS3, the 54-residue NS4A protein is required for ... See full document
8
Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.
... (G40; NS3 specific), 2133 to 2480 (B10; reacts with NS3, helicase region), 2396 to 2414 (a2396; NS4A specific), 2890 to 3192 (62D; NS5A specific), and 3409 to 3692 (B3B; NS5B ...(57). Processing ... See full document
11
Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
... Amino acids substitutions to asparagine at both the P1 and P1' positions of the putative 3/4A, 4A/4B, 4B/5A cleavage sites of the nonstructural precursor polyprotein were independently i[r] ... See full document
5
Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase.
... complete NS3-4A-4B-5A-5B open reading frame, was described recently ...site serine residue within the NS3 proteinase domain by alanine ...the NS3/4A cleavage site, ... See full document
8
Chimeric Sindbis viruses dependent on the NS3 protease of hepatitis C virus.
... a C protein inactivated by alanine substitution of the catalytic serine (21) and containing eight additional amino acid substi- tutions resulting in the transformation of the Sindbis virus ... See full document
11
Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... purified NS3 helicases containing an Ala substitution for either R393 or F438 do not unwind RNA, even though they retain some ability to unwind DNA and retain the same level of nucleic acid stimulated- ATPase ... See full document
8
Activity of purified hepatitis C virus protease NS3 on peptide substrates.
... 8 C within 20 to 30 min, and cells were grown at 22 8 C for a further 3 h, collected by centrifugation, washed with phosphate-buffered saline (20 mM sodium phosphate [pH ...8 C; 10 ml of resin ... See full document
7
Expression and identification of hepatitis C virus polyprotein cleavage products.
... The predicted sequences of the HCV H strain El polyprotein residues 192 to 383 [30] and E2 polyprotein residues 384 to -750 [30] proteins contain 5 and 9 potential acceptor sites, respec[r] ... See full document
11
Subcellular Localization, Stability, andtrans-Cleavage Competence of the Hepatitis C Virus NS3-NS4A Complex Expressed in Tetracycline-Regulated Cell Lines
... against NS3 was revealed by TXR-conjugated streptavidin, and the reactivity of antibodies against cellular marker proteins was revealed by FITC-conju- gated secondary antibodies, as shown in ...expected, ... See full document
12
Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
... vaccinia virus NPH-II heli- case, or HCV NS3 helicase (38), for which there is no evidence for dimerization or ...HCV NS3 helicase (19, 25), support the published structure of the NS3 ... See full document
10
Multiple Enzymatic Activities Associated with Recombinant NS3 Protein of Hepatitis C Virus
... full-length NS3 protein. For the production of large amounts of FL NS3 protein (amino acids 1027 to 1657), we devised a method involving expression and purification from ... See full document
12
Nuclear localization of the NS3 protein of hepatitis C virus and factors affecting the localization.
... vaccinia virus (vTF7-3) (9). To express carboxy-truncated NS3 alone (NS3DC1459; aa 1027 to 1459), the corresponding genomic sequence was amplified by PCR from pLns23/810-1463 with primers NS3-Eco-1 ... See full document
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