[PDF] Top 20 Loop Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species

... acid amplification method termed loop-mediated isothermal amplification (LAMP) has been de- veloped ...This method amplifies DNA with high specificity, sensitivity, and ... See full document

... T he genus Capripoxvirus, in the family Poxviridae, comprises three closely related species, i.e., goatpox virus (GPV), sheep- pox virus (SPV), and lumpy skin disease virus (LSDV), so named after the animal ... See full document

... In China, PED was firstly occurred in Shanghai in 1973. So far, PEDV has been observed on most swine breeding farms in most provinces since late 2010 in China [5].The economic losses caused by PEDV infec- tion have been ... See full document

... excellent detection limit, taking into account the simplicity and the rapidity of the assay (⬃35 min) compared to other DNA ...this method requires rigorous evalu- ...acid amplification assays in ... See full document

... a titrated EV71 or CVA16 strain. The specific assay showed there were no cross-reactions with Coxsackievirus A (CVA) viruses (CVA 2, 4, 5, 7, 9, 10, 14, and 25), Coxsackievirus B (CVB) viruses (CVB 1, 2, 3, 4, and 5) or ... See full document

... A loop-mediated isothermal ampli�cation (LAMP) assay targeting the arcC gene of ...PCR method that targets spa and arcC ...a detection limit of ... See full document

... In comparison with the traditional technique based on virus isolation in embryonated chicken eggs, LAMP can directly detect NDV from samples and would be relatively inexpensive and efficient. Virus isolation in ... See full document

... Acanthamoeba species may act as carriers of Cryptosporidium and Toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these ...a loop mediated isothermal ... See full document

... Theoretically, Bst polymerase used in LAMP has a higher tolerance of inhibitors than Taq polymerase used in qPCR [63, 64]. This suggests that the sensitivity of LAMP is slightly higher than that of qPCR, which is use- ... See full document

... nostic method for the detection of AIVs, the results are accurate and reliable, but the procedure is time consuming labor intensive to inoculate embryonated chicken egg for 2–5 days and harvest allantoic ... See full document

... LAMP method, such as visual detection of amplified product by add- ing calcein (14) or hydroxynaphthol blue (HNB) (15), which still require no other equipment and avoid aerosolized nucleic acid pollution, ... See full document

... forward loop pri- mers [15]. The method has been a valuable tool for the rapid diagnosis of infectious diseases in hospital labora- tories and for the rapid detection of pathogenic ... See full document

... To evaluate the specificity of the LAMPAuris primer set toward C. auris, a panel of 63 strains consisting of 39 species, including 21 filamentous fungi and 18 yeasts, were tested (Tables 2 and 3). For each of the ... See full document

... A rapid field diagnosis of KHV in common and koi carp was developed using loop- mediated isothermal amplification ...under isothermal conditions using a simple water ...two ... See full document

... Methods: We used DNA barcode-based loop-mediated isothermal amplification (LAMP) 20.. method with species-specific LAMP primers targeting the internal transcribed space[r] ... See full document

... Pseudomonas aeruginosa is a major opportu- nistic pathogen found in field trauma hospitals. It has a high prevalence of antibiotic-resistant strains, which can lead to sepsis and even death if not treated with ... See full document

... the detection limit of LAMP was approxi- mately 100 times lower than that of conventional ...for detection, even using the crude DNA obtained from boiled ...LAMP detection, since this ... See full document

... loaloop-mediated isothermal amplification (LAMP) assay compared to the standard quantitative PCR (qPCR) method, 93 midday dried blood spots on filter paper (Whatman ... See full document

... LAMP method is based on autocycling strand displace- ment DNA synthesis performed by the Bst DNA polymerase enzyme and was first described by Notomi et ...requires isothermal strand displace- ment and the ... See full document

... A total of 81 tissue samples were collected from chick- ens suspected of ALV infection, which had died from hemangioma or dramatically weight loss. These samples were detected by the LAMP assay and by PCR and virus ... See full document