[PDF] Top 20 Microbial DNA Typing by Automated Repetitive Sequence Based PCR
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Microbial DNA Typing by Automated Repetitive Sequence Based PCR
... the automated system con- serves work space, while the web-based interface enables re- mote access and data sharing for the assessment of population profiles within and among ...molecular typing is ... See full document
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Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed Field Gel Electrophoresis for Clonal Characterization of Methicillin Resistant Staphylococcus aureus
... DL typing is very rapid and relatively easy to perform (24, 26, 31, ...DL typing has a high throughput and provides results within 1 day. Based on the kit format and web-based software, the DL ... See full document
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Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes
... days. DNA was extracted from a spore and mycelial mass (a minimum of 2 square centimeters) by using an UltraClean Microbial DNA isolation kit (Mo Bio Laboratories, Solana Beach, CA) in accordance ... See full document
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Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR
... rep-PCR DNA fingerprinting. The extracted DNA was amplified using the DiversiLab Aspergillus DNA fingerprinting kit (Bacterial Barcodes, ...genomic DNA, 2.5 U of AmpliTaq, and 1.5 l of ... See full document
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Repetitive Sequence Based PCR versus Pulsed Field Gel Electrophoresis for Typing of Enterococcus faecalis at the Subspecies Level
... genomic DNA of the organ- isms by visual inspection without complicated mathematical calculation ...their PCR patterns differed by no more than two bands ...by PCR than by PFGE and the fact that some ... See full document
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Suitability of Repetitive DNA Sequence Based PCR Fingerprinting for Characterizing Epidemic Isolates of Salmonella enterica Serovar Saintpaul
... of microbial characterization are still useful, newer methods based on the molecular analysis of the genome have become important for the epidemiological characterization of patho- ...primed PCR ... See full document
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Comparison of Five Repetitive Sequence Based PCR Typing Methods for Molecular Discrimination of Salmonella enterica Isolates
... difference in one high-intensity band at 1,200 bp was observed among the serotype Typhimurium strains tested, which, how- ever, did not correlate with the two varieties in this serotype. The genetic diversity (stability) ... See full document
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Typing of Nonencapsulated Haemophilus Strains by Repetitive Element Sequence Based PCR Using Intergenic Dyad Sequences
... This method was rapid (6 h for amplification and electro- phoresis) and easy to perform. It did not require specific and expensive equipment. The banding patterns obtained were simple to read and to compare visually or ... See full document
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Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak
... rep-PCR DNA fingerprinting. DNA was extracted from a 10-l loopful of a Serratia marcescens colony using an UltraClean Microbial DNA isolation kit (Mo Bio Laboratories, Carlsbad, CA) ... See full document
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Staphylococcus aureus Genotyping Using Novel Real Time PCR Formats
... to microbial genotyping is to make use of sets of single-nucleotide polymorphisms (SNPs) in combination with binary ...multilocus sequence typing database and define 61 genotypes and the major clonal ... See full document
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Comparison of a Semiautomated Commercial Repetitive Sequence Based PCR Method with Spoligotyping, 24 Locus Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat Typing, and Restriction Fragment Length Polymorphism Based Analysis of IS6110 for Mycobacterium tuberculosis Typing
... strain typing (5, 6). Two PCR-based methods, spoligotyping and mycobacterial in- terspersed repetitive-unit–variable-number tandem-repeat typ- ing (MIRU-VNTR), which can be performed with very ... See full document
5
Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species
... days. DNA was extracted from a 10- l loop of each fungal mass using the UltraClean microbial DNA isolation kit (Mo Bio Laboratories, Solona Beach, CA), extending the bead beat step to 30 min as ... See full document
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Molecular Genotyping of Staphylococcus aureus Strains: Comparison of Repetitive Element Sequence Based PCR with Various Typing Methods and Isolation of a Novel Epidemicity Marker
... Rep-PCR typing appears to be superior to AP-PCR, since the rep-PCR results obtained in this study are highly repro- ducible due to the high annealing conditions of the ...rep-PCR ... See full document
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Principles and Applications of Methods for DNA Based Typing of Microbial Organisms
... technique based on the selective amplification of a subset of DNA fragments gener- ated by restriction enzyme digestion ...bacterial typing (21, 26, 35, 36, ...bacterial DNA is extracted, ... See full document
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Comparison of Different PCR Approaches for Typing of Francisella tularensis Strains
... species. Repetitive element sequence-based PCR (rep-PCR) is a group of methods which generate DNA fingerprints that allow discrimination between bacterial ...of repetitive ... See full document
7
Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System to Pulsed Field Gel Electrophoresis for Analysis of Outbreaks of Methicillin Resistant Staphylococcus aureus
... strain typing have been sought by clinical laboratories. PCR-based methods are attractive because many laboratories with molecular capa- bility already have the necessary equipment, trained ... See full document
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Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System with Pulsed Field Gel Electrophoresis for Molecular Typing of Vancomycin Resistant Enterococcus faecium
... Molecular typing methods are needed to distinguish prevalent strains from ep- idemic ...molecular typing of VRE chro- mosomes because of its high discriminative power and estab- lished interpretative ... See full document
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Efficacy of Pulsed Field Gel Electrophoresis and Repetitive Element Sequence Based PCR in Typing of Salmonella Isolates from Assam, India
... PFGE. For PFGE, the CDC (Centers for Disease Control and Prevention) PulseNet protocol (www.cdc.gov/ pulsenet/pathogens/protocols.html) was followed. Restriction enzyme XbaI (Fermentas Life Sciences) 40U was used for ... See full document
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Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive Sequence Based PCR for Characterization of Staphylococcus aureus Strains
... rep-PCR. DNA from a 10-l loopful of a ...Clean microbial DNA isolation kit (Mo Bio Laboratories, Solana Beach, ...Genomics. DNA genomic integrity was visualized using agarose gel ... See full document
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Typing of Listeria monocytogenes Strains by Repetitive Element Sequence Based PCR
... (1). DNA bands detected by computer were carefully verified by visual examination to correct unsatisfactory ...between DNA fingerprints were calculated with the band-matching Jaccard coefficient (S J ) ... See full document
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