[PDF] Top 20 Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
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Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... of human immunodeficiency virus type 1 (HIV-1) infection, such as the CD4 1 lymphocyte count, p24 antigenemia, and quantitative plasma and peripheral blood ... See full document
7
Multicenter Comparison of Roche COBAS AMPLICOR MONITOR Version 1 5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex Version 3 0 for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... a multicenter compara- tive evaluation of COBAS AMPLICOR HIV-1 MONITOR version ...HIV-1 RNA QT with Extractor (NucliSens) (Organon Tek- nika, Durham, ...HIV RNA version ...the ... See full document
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Intra Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... HIV-1 RNA concen- trations to panel materials. In a previous comparison of the AMPLICOR HIV-1 Monitor and NASBA assays, both meth- ods produced estimates below the nominal copy numbers ... See full document
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Quantification of Genital Human Immunodeficiency Virus Type 1 (HIV 1) DNA in Specimens from Women with Low Plasma HIV 1 RNA Levels Typical of HIV 1 Nontransmitters
... PCR methods described here, we examined HIV-1 DNA levels in endocervical and vaginal secretions from antiretroviral-naı¨ve ...low plasma HIV-1 RNA levels, would exhibit lower levels of ... See full document
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Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... therapy, human immunodeficiency virus type 1 (HIV-1) RNA levels in plasma have been dramatically reduced, frequently to below the limit of quantitation (400 ... See full document
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An enhanced sensitivity branched DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma
... HIV-1 RNA levels as statistically significant for tests in batch and real-time modes, ...between quantification values mea- sured with the Quantiplex HIV RNA ...the quantification ... See full document
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Comparative evaluation of NASBA HIV 1 RNA QT, AMPLICOR HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... HIV-1 RNA assay can be ...viral RNA isolation procedure is less time-consuming than the gua- nidinium extraction method used by NASBA and AMPLICOR but uses a large amount of plasma, which is a ... See full document
7
Comparison of Two Amplification Technologies for Detection and Quantitation of Human Immunodeficiency Virus Type 1 RNA in the Female Genital Tract
... tion methods can be used to arrive at comparable determinations of HIV-1 RNA copy number in the female genital ...blood plasma (3), we observed differences in estimated RNA copy number ... See full document
5
Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... HIV-1 RNA sample ...The RNA extraction reagent was prepared by mixing ...of plasma was added to each ...of plasma) of aqueous phase was mixed with 450 m l of ...the RNA and ... See full document
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Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV 1) RNA bDNA v3 0, Cobas AmpliPrep/Cobas TaqMan HIV 1, and NucliSens HIV 1 EasyQ v1 2, Testing HIV 1 Non B Subtypes and Recombinant Variants
... that human immunodeficiency virus type 1 (HIV-1) viremia assays are applicable to all distinct ...of three commercial viral load assays—Versant HIV-1 ... See full document
6
Human Immunodeficiency Virus Type 1 Drug Resistance Testing: a Comparison of Three Sequence Based Methods
... Two major molecular phenotypic assays are commercially available and have been used in clinical trials and outcome studies. Antivirogram (Virco, Mechelen, Belgium) and Pheno- Sense (ViroLogic, South San Francisco, ... See full document
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Detection and Quantification of Human Immunodeficiency Virus Type 1 p24 Antigen in Dried Whole Blood and Plasma on Filter Paper Stored under Various Conditions
... and quantification of HIV-1 in plasma were similar when samples were stored on filter paper and submitted to an HD p24 Ag assay and when they were stored frozen in polypropylene tubes and evaluated ... See full document
5
Highly Sensitive Methods for Quantitation of Human Immunodeficiency Virus Type 1 RNA from Plasma, Cells, and Tissues
... in plasma (3, ...of human immunodeficiency virus (HIV) RNA in plasma is widely used to monitor HIV type 1 (HIV-1) levels ...in plasma (7, ...the ... See full document
5
Ten Years of External Quality Assessment of Human Immunodeficiency Virus Type 1 RNA Quantification
... with human immunodefi- ciency virus type 1 ...molecular quantification of HIV-1 RNA was intro- duced by the United Kingdom National External Quality Assessment Service for ... See full document
6
Impact of Human Immunodeficiency Virus Type 1 (HIV 1) Genetic Diversity on Performance of Four Commercial Viral Load Assays: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR v1 5, VERSANT HIV 1 RNA 3 0, and NucliSens HIV 1 QT
... Human immunodeficiency virus type 1 (HIV-1) evolution and changing strain distribution present a chal- lenge to nucleic acid-based ...accurate quantification of ... See full document
9
Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
... viral RNA in DPSs collected under severe tropical ...HIV-1 RNA has been successfully quanti- fied from DPSs collected in the Middle East, stored for 2 to 3 days at ambient temperatures, and then ... See full document
7
Multicenter Evaluation of Methods To Quantitate Human Immunodeficiency Virus Type 1 RNA in Seminal Plasma
... (Fig. 1 [panels for laboratories C, E, and ...of type of body fluid matrix on the measurement of HIV-1 ...HIV-1 RNA copies per milliliter obtained from the spiked seminal plasma ... See full document
6
Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing
... that RNA quan- tifications from DPS and DBS were still possible after 7 to 15 days at 37°C (1, 11, 15) and after 3 weeks to 1 year at room temperature (10, ...periods. Plasma viral loads for a ... See full document
12
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... TaqMan human immunodeficiency virus (HIV) (HPS-CTM HIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in ... See full document
5
Design and Evaluation of a Human Immunodeficiency Virus Type 1 RNA Assay Using Nucleic Acid Sequence Based Amplification Technology Able To Quantify Both Group M and O Viruses by Using the Long Terminal Repeat as Target
... The human immunodeficiency virus type 1 (HIV-1) RNA level in plasma or serum has become one of the most impor- tant markers for monitoring HIV-1-infected ... See full document
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