[PDF] Top 20 Use of Multiple Competitors for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
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Use of Multiple Competitors for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... of RNA competitors with different copy numbers covering the common interval in HIV-1 viremias (19) were ...of RNA competitors were designed to have a minor overlap in copy ... See full document
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Comparative evaluation of NASBA HIV 1 RNA QT, AMPLICOR HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... HIV-1 RNA assay can be ...to use; ...viral RNA isolation procedure is less time-consuming than the gua- nidinium extraction method used by NASBA and AMPLICOR but uses a large amount of ... See full document
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Intra Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... Table 1 are the differences among the five assays. The ES-bDNA assay for HIV-1 RNA is a second- generation assay developed by the Chiron Corporation (Em- eryville, ...the use of four different ... See full document
5
An enhanced sensitivity branched DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma
... The quantification of human immunodeficiency virus type 1 (HIV-1) RNA has facilitated clinical research and expedited the development of antiretroviral ...the ... See full document
7
Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... the use of reconstructed HIV-1-infected plasma, 21 cross-sectionally collected clinical samples obtained from HIV-1-infected individuals, and longitudinally collected samples obtained from ... See full document
7
Ten Years of External Quality Assessment of Human Immunodeficiency Virus Type 1 RNA Quantification
... with human immunodefi- ciency virus type 1 ...molecular quantification of HIV-1 RNA was intro- duced by the United Kingdom National External Quality Assessment Service for ... See full document
6
Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
... HIV-1 RNA on filter paper has far-reaching epidemiological and public health im- ...plications. Quantification of RNA levels with DPSs will be particularly valuable in the neonatal setting, in ... See full document
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Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... HIV-1 RNA sample ...The RNA extraction reagent was prepared by mixing ...of plasma was added to each ...of plasma) of aqueous phase was mixed with 450 m l of ...the RNA and ... See full document
5
Multicenter Evaluation of Methods To Quantitate Human Immunodeficiency Virus Type 1 RNA in Seminal Plasma
... HIV-1 RNA isola- tion. Instead, viral RNA was isolated using the silica bead procedure of Boom et ...seminal plasma specimens inhibited RT-PCR amplification and that these inhibitors were ... See full document
6
Detection and Quantification of Human Immunodeficiency Virus Type 1 p24 Antigen in Dried Whole Blood and Plasma on Filter Paper Stored under Various Conditions
... the plasma HIV-1 RNA load was relatively weak, for reasons not fully elucidated in our ...receptor 1 (CD35), also called the immune adherence receptor, on primate erythrocytes ...HIV-1 ... See full document
5
Evaluation of a quality assurance program for quantitation of human immunodeficiency virus type 1 RNA in plasma by the AIDS Clinical Trials Group virology laboratories
... in plasma HIV-1 RNA levels averaged over the dy- namic range of the ...patient plasma specimens. An accurate measurement of HIV-1 RNA copy number is also necessary for defining ... See full document
7
Genetic Characterization of Rebounding Human Immunodeficiency Virus Type 1 in Plasma during Multiple Interruptions of Highly Active Antiretroviral Therapy
... rebound plasma HIV by ...on plasma HIV env (V3-V5) se- quences obtained from the 4th week of each sequential treat- ment ...HIV plasma RNA to perform the necessary amplification during each ... See full document
9
Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... of plasma human immunodeficiency virus type 1 (HIV-1) RNA levels has become an important tool for identifying individuals likely to benefit from antiretroviral ... See full document
6
Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... man immunodeficiency virus (HIV)-infected patients ...HIV RNA in plasma, including (i) the Cobas TaqMan HIV type 1 (HIV-1) test (Roche Molecular Systems, Basel, Swit- ... See full document
6
Genotypic Changes in Human Immunodeficiency Virus Type 1 Associated with Loss of Suppression of Plasma Viral RNA Levels in Subjects Treated with Ritonavir (Norvir) Monotherapy
... in plasma virus RNA levels and rapid development of the V82A mutation in the protease ...require multiple, independent ge- netic changes in a single genome in order to completely escape viral ... See full document
11
Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1 1)
... the use of a first ultracen- trifugation step with a large volume of ...HIV-2 RNA recognized in the few patients carrying subtype B inversely correlated with their CD4 cell counts and clinical symptoms, ... See full document
5
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... practice. Quantification of HIV-1 RNA with this assay requires two sample preparation procedures: in the first, ultrasensitive method, 500 l of plasma is centrifuged to increase the input of ... See full document
5
Quantification of Genital Human Immunodeficiency Virus Type 1 (HIV 1) DNA in Specimens from Women with Low Plasma HIV 1 RNA Levels Typical of HIV 1 Nontransmitters
... For genital specimens and for mock swabs, HIV-1 pol real-time reactions were performed in duplicate. For genital specimens, HIV-1 pol duplicate reactions were considered acceptable only if the readout ... See full document
6
Design and Evaluation of a Human Immunodeficiency Virus Type 1 RNA Assay Using Nucleic Acid Sequence Based Amplification Technology Able To Quantify Both Group M and O Viruses by Using the Long Terminal Repeat as Target
... available human immunodeficiency virus type 1 (HIV-1) RNA quantification assays can detect most viruses of the group M subtypes, but a substantial number are missed ... See full document
6
Lentivirus-Specific Cytotoxic T-Lymphocyte Responses Are Rapidly Lost in Thymectomized Cats Infected with Feline Immunodeficiency Virus
... example, immunodeficiency-causing lentiviruses, such as human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus, and feline immuno- ... See full document
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