[PDF] Top 20 A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid resistant Aedes aegypti
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A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid resistant Aedes aegypti
... DNA PCR product before gel electrophoresis and thus reduced product ...this multiplex PCR method, the results were still interpretable by using these oversized bands, instead of true ...for ... See full document
9
Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand
... permethrin resistance when they are effectively homozygote, ...three mutations in the homozy- gote as computer models have indicated that pyrethroids preferentially bind to the open state of VGSC by inter- ... See full document
7
First report of V1016G and S989P knockdown resistant (kdr) mutations in pyrethroid resistant Sri Lankan Aedes aegypti mosquitoes
... of resistance was observed in the present study for ...real resistance to rec- ommended dosages at the current study ...kdr mutations in pyrethroid-resistant ... See full document
6
Larval density mediates knockdown resistance to pyrethroid insecticides in adult Aedes aegypti
... Ae. aegypti population dynamics ...Ae. aegypti life-cycle, which in- volves four free-living aquatic larval stages and a pupal stage that does not feed, occurs mainly in man-made con- tainers such as ... See full document
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Knockdown resistance in the dengue vector Aedes aegypti and its implications for Wolbachia Aedes aegypti trials
... in resistant group, ...of pyrethroid insecticides to control mosquitoes and other insects of public health importance, both at a large scale as authorized by local government and in household usage, is ... See full document
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Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia
... of resistance to insecticides in the two major dengue vectors in Malaysia, this study has provided suitable information for the design and implementation of successful resistance management strategies ... See full document
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Detection of the V1016G mutation in the voltage gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand
... in resistant or susceptible ...Ae. aegypti harbor either the 1016G mutation, or the more common and widespread 1534C ...intensive pyrethroid usage through- out ...Ae. aegypti, especially using ... See full document
10
Combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of Aedes aegypti from Saudi Arabia
... Ae. aegypti strain from Vietnam [40], which, together with overexpression of the strongly pyrethroid-metabolising gene CYP9J32 [14] relative to the susceptible Bora Bora strain, was interpreted as evidence ... See full document
10
Combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of Aedes aegypti from Saudi Arabia
... Ae. aegypti strain from Vietnam [40], which, together with overexpression of the strongly pyrethroid-metabolising gene CYP9J32 [14] relative to the susceptible Bora Bora strain, was interpreted as evidence ... See full document
10
Pyrethroid resistance in the dengue vector Aedes aegypti in Southeast Asia: present situation and prospects for management
... of pyrethroid susceptibility in Aedes aegypti in Southeast Asia Published literature with regards to the insecticide resist- ance profiles of ...Ae. aegypti against pyrethroids in most of the ... See full document
17
Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin
... a multiplex PCR format, it has inherent capacity for expansion to detect other antibiotic resistant ...significant mutations with alternative fluorescent Taqman ...the resistant and ... See full document
12
Pyrethroid Resistance in Malaysian Populations of Dengue Vector Aedes aegypti Is Mediated by CYP9 Family of Cytochrome P450 Genes
... Summary Aedes aegypti is the major vector of emerging or re-emerging arboviruses such as dengue, chikungunya and Zika viruses ...ing resistant to commonly used insecticides in several regions across ... See full document
20
Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China
... Ae. aegypti is present in many residential areas of Southern ...Ae. aegypti have become resistant to pyrethroid insecticides [18, 19], but the underlying mechanism responsible for this re- ... See full document
9
Detection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
... EMB resistant clinical isolates of ...several mutations in these genes, especially in embB, and up to 90% of them were found in codon 306 ...its resistance development is not generally very high, ... See full document
5
Gene Amplification, ABC Transporters and Cytochrome P450s: Unraveling the Molecular Basis of Pyrethroid Resistance in the Dengue Vector, Aedes aegypti
... Ae. aegypti genome was used to compare gene expression in the two Caribbean populations with a standard lab susceptible ...Quantitative PCR analysis of selected genes from the microarray ...the ... See full document
10
Knockdown resistance (kdr) like mutations in the voltage gated sodium channel of a malaria vector Anopheles stephensi and PCR assays for their detection
... Background: Knockdown resistance (kdr) in insects, resulting from mutation(s) in the voltage-gated sodium channel (vgsc) gene is one of the mechanisms of resistance against DDT and ... See full document
7
Insecticide resistance status of Aedes aegypti and Aedes albopictus mosquitoes in Papua New Guinea
... assessing Aedes populations for insecticide resistance in Madang and Port Moresby, located on the north and south coasts of PNG, ...Ae. aegypti and 768 ...Ae. aegypti and 48 Ae. albopictus) ... See full document
8
Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... Rapid detection of the drug resistance of ...detect-resistant mutations, real-time PCR has the advantages of rapidity, high sensitivity, reproducibility, and a low risk of ...real-time ... See full document
7
Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids
... ACKGROUND: Aedes aegypti is a vector of several pathogens including dengue ...Ae. aegypti in Yogyakarta Province, ...with pyrethroid resistance constitute one of the main causative ... See full document
6
Characterisation of mechanisms of insecticide resistance in Malaysian populations of the arbovirus vectors Aedes aegypti and Aedes albopictus
... kdr mutations in ...kdr mutations in ...The PCR amplification successfully generated a PCR product of 2586bp as expected for 12 tested cDNA samples all DDT ... See full document
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