[PDF] Top 20 Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase
Has 10000 "Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase" found on our website. Below are the top 20 most common "Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase".
Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase
... and C-terminal trunca- tions in NS3 were made, and the fragments were cloned into ...the helicase domain, which includes conserved residues important for NTP-bind- ing, NTPase, and helicase ... See full document
10
Hepatitis C Virus Core Protein Interacts with Cellular Putative RNA Helicase
... the virus, such as translation of HCV polyprotein or replication of the HCV RNA ...simplex virus or adenovirus, redistribution of small nuclear ribonucleoprotein particle or SR proteins occurs in ... See full document
13
Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... that affect RNA helicase activity and the ...ATPase activity is abol- ished will also impede RNA helicase ...ATPase activity (6 to 16% of the wild-type helicase) still ... See full document
9
NS3 Helicase Domains Involved in Infectious Intracellular Hepatitis C Virus Particle Assembly
... in virus assembly has not been suspected previously for HCV, several studies have suggested that the related NS3 proteins of classical flaviviruses, including both KUN and YFV (13, 20, 27), as well as pestiviruses ... See full document
16
Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
... bined helicase and nucleoside triphosphatase (NTPase), and an RNA-dependent RNA polymerase, are believed to be com- ponents of a complex responsible for viral RNA ...The C-terminal 450 amino acids of the ... See full document
10
The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase
... NS3 helicase activity and ATP-coupled RNA binding to NS3 are essential for the function of ...NS3 helicase activity during viral replication (1, 8, 19), no studies have directly linked RNA ... See full document
8
The NS3 Helicase and NS5B-to-3′X Regions Are Important for Efficient Hepatitis C Virus Strain JFH-1 Replication in Huh7 Cells
... colony formation as- says are time-consuming to perform and it is difficult to eval- uate precise replication levels using this ...colony formation efficiency ...luciferase activity of approxi- ... See full document
11
Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... NS3 helicase region of a hepatitis C virus (HCV) Con1 subgenomic replicon to ascertain the role of the helicase in viral ...NS3 helicase ( ⌬ hel), and six others contained one of ... See full document
8
Roles of the AX4GKS and Arginine-Rich Motifs of Hepatitis C Virus RNA Helicase in ATP- and Viral RNA-Binding Activity
... GR-1493), respectively (Fig. 1A; data not shown), and no ad- ditional mutations were generated during the cloning proce- dures. The overexpressed NS3 proteins were found predomi- nantly in the insoluble fractions ... See full document
6
Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone
... with formation of infectious virus production and ...protease, helicase and polymerase) of HCV ...protease, helicase and poly- merase activity and thereby inhibit intracellular HCV ... See full document
12
Mutational analysis of the hepatitis C virus RNA helicase.
... unwanted mutations in unsequenced ...not affect the enzymatic activ- ity of the NS3H ...zymatic activity, and the enzyme activity of the purified NS3H protein was inhibited by NS3-specific ... See full document
10
Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
... NTPase activity. NS3FL was found to exhibit a substan- tially higher helicase activity than NS3H, but it did not affect the level of its NTPase activity ...the helicase without ... See full document
11
Mutations in the helicase-like domain of protein 1a alter the sites of RNA-RNA recombination in brome mosaic virus.
... might affect the incidence of nontemplated nucleotide addition, which could occur while the polymerase waits for an opportunity to progress through downstream regions of the ...influenza virus RNA ... See full document
10
Regulating Intracellular Antiviral Defense and Permissiveness to Hepatitis C Virus RNA Replication through a Cellular RNA Helicase, RIG-I
... adaptive mutations and replicate autono- mously, albeit with different efficiencies, when introduced into cultured human hepatoma (Huh7) cells (L2198S, HP, and K2040; ...promoter activity, whereas only ... See full document
11
Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... to helicase trig- gers the close of the intercleft domain between domains 1 and 2 and thereby translocates domain 1 in the 3⬘-to-5⬘ direction along with a bound substrate ...the helicase as a ...would ... See full document
12
DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication
... might affect the function of HIV-1 Rev when both proteins were ...luciferase activity in 293FT cells transfected with this reporter plasmid was stimulated by Rev, which induced a fourfold increase in the ... See full document
5
Identification of Inhibitor against Zika Virus NS3 Helicase
... the virus wherein antibodies against ZIKV have been detected in numerous other animal species including water buffalo, elephants, goats, hippos, impala, lions, sheep, rodents, and ...Zika virus, which are: ... See full document
9
Structural studies of Helicase NS3 variants from Hepatitis C virus genotype 3 in virological sustained responder and non responder patients
... Natural occurrence of amino acids substitution in non- responder HCV patients has been explored for impor- tant sites in relation to the perfect excise of helicase function. Correlations of the variants L210E and ... See full document
8
NTPase and 5′ to 3′ RNA Duplex-Unwinding Activities of the Hepatitis E Virus Helicase Domain
... NTPase/ helicase domain and its mutants. Full-length HEV NTPase/ helicase (amino acids 960 to 1204 of the ORF1, 245 amino acids long) (24) was expressed as an N-terminal histidine tagged fusion protein in a ... See full document
8
Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3′-Terminal Sequences of Viral Positive- and Negative-Strand RNA
... large open reading frame that encodes a polyprotein of ap- proximately 3,000 amino acids, which is subsequently proteo- lytically processed by cellular signal peptidases and two HCV- encoded proteases to produce mature ... See full document
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