[PDF] Top 20 Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation
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Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation
... and gag was cloned from pSVR into pGEM7Zf1 (Promega), creating ...HXBc2 p2 and NC domain with 15 bp of HIV-2 sequence at the 59 and 39 ...NC domain flanked by 30 bp of HIV-2 ... See full document
9
A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... homologous RNA molecules that are tightly associated through numerous interactions (7, 38, ...of RNA extracted from virions revealed that these RNA molecules are joined noncovalently at a discrete ... See full document
7
Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
... produce Gag proteins, they only transcribed viral RNA available for packaging by the wild-type Gag pro- teins from ...the possible preferential synthesis of the shorter ⌬DIS ... See full document
14
Cytoplasmic Utilization of Human Immunodeficiency Virus Type 1 Genomic RNA Is Not Dependent on a Nuclear Interaction with Gag
... in Gag for gRNA encapsidation. The codon-optimized gag/gag-pol expression plasmid Hgp syn allows the production of constant high levels of Gag/Gag-Pol, irrespec- tive of whether ... See full document
13
Roles of Matrix, p2, and N-Terminal Myristoylation in Human Immunodeficiency Virus Type 1 Gag Assembly
... produce Gag VLP from the cell surface (4, 16, ...in Gag-Gag interaction, however, as non- myristoylated Gag protein may be rescued into budding Gag VLP by a relatively low level of ... See full document
8
The Major Human Immunodeficiency Virus Type 2 (HIV-2) Packaging Signal Is Present on All HIV-2 RNA Species: Cotranslational RNA Encapsidation and Limitation of Gag Protein Confer Specificity
... cotranslational packaging mechanism by wild- type HIV-2 would seemingly preclude it from use as a gene vector ...mutant RNA to efficiently capture newly made cognate Gag ...The ... See full document
12
Effects of Mutations in the Human Immunodeficiency Virus Type 1 gag Gene on RNA Packaging and Recombination
... genomic RNA is selectively encap- sidated into virions through interactions between RNA and the Gag polyproteins (15, 20, ...38). Human immunodeficiency virus type ... See full document
7
Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2.
... the role in HIV-2 packaging of sequences located immediately upstream and downstream of the 59 major splice ...the gag gene of HIV-2 make only a minor contribution to packag- ing and ... See full document
5
Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.
... its role in packaging and dimer ...c RNA by sequence analysis and nuclease accessibility mapping (2, 10, 19, 34); based on such data, we have proposed (10) a working model of the c locus that ... See full document
8
Binding of the Human Immunodeficiency Virus Type 1 Gag Protein to the Viral RNA Encapsidation Signal in the Yeast Three-Hybrid System
... HIV Gag with HIVC reflects binding in vivo, the response should be affected by mutations in both Gag and ...the Gag polyprotein is sufficient for binding in vitro (reviewed in reference ...whether ... See full document
6
Possible Role of Dimerization in Human Immunodeficiency Virus Type 1 Genome RNA Packaging
... the packaging and dimerization functions (Fig. 2 and ...for packaging, whereas we observed re- gions functional for packaging but not dimerization ...dimerized RNA strands do not need ... See full document
10
Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging
... major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA ...the role of NC in the selective recognition of ... See full document
11
Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RNA
... of Gag and HIV-1 RNA association in vivo A previously described protocol with modifications was used ...containing 1% formaldehyde and incubated for 10 min at room tem- perature to cross-link ... See full document
12
RNA Structure and Packaging Signals in the 5′ Leader Region of the Human Immunodeficiency Virus Type 1 Genome
... target RNA into HIV-1 virions ...mous packaging activity spans the first 350 to 400 contiguous bases of the genome, and thus includes not only SL1 to SL4 but also 242 bases upstream (22, 25, 31, ... See full document
7
The human immunodeficiency virus type 1 encapsidation site is a multipartite RNA element composed of functional hairpin structures.
... p D 42 was created by PCR amplification of a fragment of pMSMBA using sense primer 275-298 (5 9 -CCTAGCATTTCGTCACATGGCCCG) and mis- match antisense primer 688-660 MluI (5 9 -CGAGAGATCTCCTCACGCGTT ACTTTCGC) to create an ... See full document
11
The Nucleocapsid Region of Human Immunodeficiency Virus Type 1 Gag Assists in the Coordination of Assembly and Gag Processing: Role for RNA-Gag Binding in the Early Stages of Assembly
... NX Gag expression or its overt stability in the cell, consistent with a severe assembly defect (43, ...NC-mediated RNA binding per se, is somehow responsible for this NX assembly ...the RNA scaffold ... See full document
10
A Heterologous, High-Affinity RNA Ligand for Human Immunodeficiency Virus Gag Protein Has RNA Packaging Activity
... subgenomic RNA species within the virions ...Table 1). HIV-gpt virions contained almost exclusively genomic RNA, indicating a high degree of ...Table 1). Virions in which SL3 was replaced by ... See full document
6
The Conformation of the Mature Dimeric Human Immunodeficiency Virus Type 1 RNA Genome Requires Packaging of Pol Protein
... of RNA packaging and dimerization is directly linked to Gag, whether in its precursor form as the Gag polyprotein or following processing by viral PR to its constit- uent proteins (2, ... See full document
10
Randomization and In Vivo Selection Reveal a GGRG Motif Essential for Packaging Human Immunodeficiency Virus Type 2 RNA
... tional role for the 3 ⬘ side of pal in vitro and in vivo. To understand the role of the 5 ⬘ side of pal, we used an in vivo randomization and selection methodology similar to one first used by Berkhout and ... See full document
9
Association of Human Immunodeficiency Virus Type 1 Vif with RNA and Its Role in Reverse Transcription
... regulatory role in reverse transcription during the entry phase of the virus’s life cycle (6, 8, 30, 35, 40, ...uncoating, virus core stability, or reverse transcription itself is being ...cell type- ... See full document
8
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