[PDF] Top 20 The Nucleoprotein Is Required for Efficient Coronavirus Genome Replication
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The Nucleoprotein Is Required for Efficient Coronavirus Genome Replication
... TGEV replication level was about threefold lower in 293T cells than in BHK- pAPN cells (data not shown), this could explain the fact that TGEV replicons were cytopathic in BHK-pAPN cells and not in 293T ... See full document
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The N-Terminal Extension of the Influenza B Virus Nucleoprotein Is Not Required for Nuclear Accumulation or the Expression and Replication of a Model RNA
... The nucleoprotein (NP) of influenza B virus is 50 amino acids longer at the N-terminus than influenza A virus NP and lacks homology to the A virus protein over the first 69 ...and replication of a model ... See full document
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Coronavirus Nucleocapsid Protein Facilitates Template Switching and Is Required for Efficient Transcription
... 1. Almazan, F., C. Galan, and L. Enjuanes. 2004. The nucleoprotein is required for efficient coronavirus genome replication. J. Virol. 78:12683–12688. 2. Chang, C. K., S. C. Sue, ... See full document
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Replication-Coupled and Host Factor-Mediated Encapsidation of the Influenza Virus Genome by Viral Nucleoprotein
... are required for the viral polymerase prior to its movement along a 12- to 19-nt-long vRNA template from the 3 ⬘ terminus of vRNA, where the hairpin loop and double-stranded promoter region are ...virus ... See full document
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The N Terminus of the Influenza B Virus Nucleoprotein Is Essential for Virus Viability, Nuclear Localization, and Optimal Transcription and Replication of the Viral Genome
... is required for optimal replication of a virus-like ...is required for optimal viral replication, constructs were created that expressed serially truncated versions of BNP ...support ... See full document
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The Surface-Exposed PA51-72-Loop of the Influenza A Virus Polymerase Is Required for Viral Genome Replication
... The genome of influenza A viruses consists of eight single-stranded negative-sense viral RNA segments which together with the viral polymerase and nucleoprotein (NP) form viral ribonucleoprotein (vRNP) ... See full document
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The Coronavirus Infectious Bronchitis Virus Nucleoprotein Localizes to the Nucleolus
... Murine hepatitis virus has been shown to replicate in enu- cleated cells (38), indicating that the nucleus is not required for coronavirus replication. However, our data have shown that the IBV N ... See full document
7
Peroxiredoxin 1 Is Required for Efficient Transcription and Replication of Measles Virus
... RNA genome and belongs to the genus Morbillivirus in the family ...proteins: nucleoprotein (N), phosphoprotein (P), matrix pro- tein (M), fusion protein (F), hemagglutinin protein (H), and large protein ... See full document
7
Sulfatide Is Required for Efficient Replication of Influenza A Virus
... IAV replication through efficient translocation of the newly synthesized IAV nucleoprotein (NP) from the nucleus to the cytoplasm, by using genetically produced cells in which sulfatide expression ... See full document
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Host Factors in Coronavirus Replication
... SARS-CoV replication, including 40 ‘proviral’ proteins that promote efficient replication (de Wilde et ...SARS-CoV replication is more extensively influenced by cellular ...controlling ... See full document
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EWSR1 Binds the Hepatitis C Virus cis-Acting Replication Element and Is Required for Efficient Viral Replication
... RNA replication cannot occur at the same time on the same pos- itive-stranded RNA viral genome (57), and the switch from one process to another must be ...RNA replication elements in the 3= of the ... See full document
10
A Point Mutation within the Replicase Gene Differentially Affects Coronavirus Genome versus Minigenome Replication
... similar genome organization ...the genome and contains two overlapping open reading frames (ORF1a and ORF1b) that are translated by a ribosomal frameshift mechanism, leading to the expression of two large ... See full document
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The 3' untranslated region of picornavirus RNA: features required for efficient genome replication.
... stem-loops. First we tested if either of the PV3 stem-loops was functionally equivalent to the single stem-loop of the HRV14 3 9 UTR by removing each of the stems individually. Very low levels of CAT activity were ... See full document
10
cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging
... Predicted stem-loop. Secondary structure analysis of the en- tire 3⬘ UTRs of the 19 U.S., European, and Australian IBV strains referred to above, plus 3 strains of TCoV (2, 11), using the software package RNAdraw (23), ... See full document
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Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication.
... is required to further process the de-capped ...during coronavirus infection the EndoU activity conceptually fulfils the same task, namely the removal of dsRNA that would otherwise trigger host cell dsRNA ... See full document
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Timely Synthesis of the Adenovirus Type 5 E1B 55-Kilodalton Protein Is Required for Efficient Genome Replication in Normal Human Cells
... T he E1B gene of species C human adenoviruses, such as adeno- virus type 5 (Ad5), encodes unrelated proteins of 19 and 55 kDa that contribute to optimizing the environment for efficient viral replication ... See full document
9
Influenza A Virus M2 Protein Apical Targeting Is Required for Efficient Virus Replication
... IAV infects and buds primarily from the apical plasma membrane during infection of polarized epithelial cells. All three viral integral membrane proteins are targeted to the apical plasma membrane (4–9). Because of this, ... See full document
17
Norovirus Genome Circularization and Efficient Replication Are Facilitated by Binding of PCBP2 and hnRNP A1
... MNV-1 genome require RNA-RNA contacts in the CS but the participation of cellular proteins is required to stabilize these ...MNV-1 genome (references 14, 17, and 45 and unpublished ...domains ... See full document
17
Transmissible Gastroenteritis Coronavirus Genome Packaging Signal Is Located at the 5′ End of the Genome and Promotes Viral RNA Incorporation into Virions in a Replication-Independent Process
... dissect replication and packaging functions, a new experi- mental approach was designed, consisting of a comparative anal- ysis of the intracellular and virion RNAs levels of the engineered RNAs in noninfected and ... See full document
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Efficient Translation Initiation Is Required for Replication of Bovine Viral Diarrhea Virus Subgenomic Replicons
... Two biotypes can be distinguished following infection of cultured cells. Viruses that do not appear to cause any cell damage are considered noncytopathic (ncp), whereas cyto- pathic (cp) isolates cause cell shrinkage, ... See full document
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