[PDF] Top 20 Phosphorylation of the UL31 Protein of Herpes Simplex Virus 1 by the US3-Encoded Kinase Regulates Localization of the Nuclear Envelopment Complex and Egress of Nucleocapsids
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Phosphorylation of the UL31 Protein of Herpes Simplex Virus 1 by the US3-Encoded Kinase Regulates Localization of the Nuclear Envelopment Complex and Egress of Nucleocapsids
... The cells were then dehydrated at 4°C using a graduated series of ethanol concentrations (20%, 50%, 70%, and 100%), a subsequent acetone-ethanol mixture (1:1), and, finally, 100% acetone. This was followed ... See full document
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Herpes Simplex Virus 1 Protein Kinase Us3 and Major Tegument Protein UL47 Reciprocally Regulate Their Subcellular Localization in Infected Cells
... a protein kinase is tightly regu- lated; ...other protein kinases, or interaction with other proteins ...activated protein kinase phosphorylates a substrate(s), and as a result, the ... See full document
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Herpes Simplex Virus 1 Induces Phosphorylation and Reorganization of Lamin A/C through the γ134.5 Protein That Facilitates Nuclear Egress
... erpes simplex virus 1 (HSV-1) replicates and packages its DNA in the cell ...the nucleocapsids traverse the nucleoplasm and cross the nuclear ...the nuclear membranes in a ... See full document
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Differences in the Regulatory and Functional Effects of the Us3 Protein Kinase Activities of Herpes Simplex Virus 1 and 2
... Us3 protein kinases encoded by herpes simplex virus 1 (HSV-1) and 2 (HSV-2) are serine/threonine protein kinases with amino acid sequences that are conserved ... See full document
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Effects of Phosphorylation of Herpes Simplex Virus 1 Envelope Glycoprotein B by Us3 Kinase In Vivo and In Vitro
... the Us3 functions de- scribed above have been ...that Us3 phosphorylates Thr-887 in the cytoplasmic tail of gB, which appears to downregulate cell surface expression of gB ...recombinant virus ... See full document
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Regulation of the Catalytic Activity of Herpes Simplex Virus 1 Protein Kinase Us3 by Autophosphorylation and Its Role in Pathogenesis
... The herpes simplex virus 1 (HSV-1) Us3 gene encodes a serine/threonine protein kinase with an amino acid sequence that is conserved in the subfamily ... See full document
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Characterization of a Herpes Simplex Virus 1 (HSV-1) Chimera in Which the Us3 Protein Kinase Gene Is Replaced with the HSV-2 Us3 Gene
... cell nuclear membrane is a unique mecha- nism by which herpesvirus nucleocapsids traverse the inner nu- clear membrane (INM) and outer nuclear membrane (ONM): progeny nucleocapsids acquire ... See full document
17
Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress
... the herpes simplex virus 1 (HSV-1) major virion structural protein UL47 (also designated VP13/14), we screened cells overexpressing UL47 for UL47-binding cellular ...cell ... See full document
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Role of the Herpes Simplex Virus 1 Us3 Kinase Phosphorylation Site and Endocytosis Motifs in the Intracellular Transport and Neurovirulence of Envelope Glycoprotein B
... dorabies virus (PRV), human cytomegalovirus (HCMV), and varicella-zoster virus (VZV) (4, 13, 18, ...proper localization and accumulation of gB in infected cells, and HSV-1 pathogenesis in vivo ... See full document
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Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... IE protein, ICP22, a 420-residue protein encoded by the Us1 gene, conserved in the subfamily Alphaherpesvirinae of the family Her- ...ICP22 regulates the expression of specific viral genes ... See full document
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Herpes Simplex Virus 1 Protein Kinase Us3 Phosphorylates Viral Envelope Glycoprotein B and Regulates Its Expression on the Cell Surface
... viral protein kinase acts in infected cells, the kinase’s physiological substrate(s) and its phosphorylation site(s) must be ...mutant virus lacking protein kinase activity also ... See full document
12
Effects of Lamin A/C, Lamin B1, and Viral US3 Kinase Activity on Viral Infectivity, Virion Egress, and the Targeting of Herpes Simplex Virus UL34-Encoded Protein to the Inner Nuclear Membrane
... cytosolic nuclear localization signals to target them past the nuclear pore membrane to the INM via a karyopherin  1-driven pathway (13, ...the nuclear lamina (4). The nuclear ... See full document
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Herpes Simplex Virus 1-Encoded Protein Kinase UL13 Phosphorylates Viral Us3 Protein Kinase and Regulates Nuclear Localization of Viral Envelopment Factors UL34 and UL31
... factor 1 ␦ (EF-1 ␦ ), casein kinase II  subunit, and RNA polymerase II have been reported to be putative substrates for UL13 (4, 10, 20, 29, 32, 37, 44, 51, 57, ...UL13-mediated ... See full document
11
Herpes Simplex Virus 1 UL47 Interacts with Viral Nuclear Egress Factors UL31, UL34, and Us3 and Regulates Viral Nuclear Egress
... that Us3 phosphorylated UL47 and promoted its nuclear localization in HSV-1-infected cells ...a Us3 kinase-dead mutant or carrying a mutation in the Us3 ... See full document
11
The Primary Enveloped Virion of Herpes Simplex Virus 1: Its Role in Nuclear Egress
... NEC protein (23), whence it follows that they disengage from the nucleocapsid as it enters the cytoplasm (see ...a nuclear sac from a US3-null ... See full document
14
Reconstitution of Herpes Simplex Virus Type 1 Nuclear Capsid Egress In Vitro
... Herpes simplex virus type 1 (HSV-1) replicates and assem- bles its capsids in the ...smaller nuclear pores, it has been assumed that the capsids could escape the nuclei only by ... See full document
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Nuclear Egress and Envelopment of Herpes Simplex Virus Capsids Analyzed with Dual-Color Fluorescence HSV1(17+)
... subcellular localization of nuclear pore complexes, capsids, and envelope proteins al- lowed us to distinguish both nuclear from cytoplasmic capsids and virions in vesicles from cytosolic ...the ... See full document
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US3 and US3.5 Protein Kinases of Herpes Simplex Virus 1 Differ with Respect to Their Functions in Blocking Apoptosis and in Virion Maturation and Egress
... expressing Us3 or BC2609 expressing ...against Us3 and the mitochondrial marker voltage-dependent anion-selective channel protein 1 ... See full document
13
Nuclear Localization and Shuttling of Herpes Simplex Virus Tegument Protein VP13/14
... a nuclear targeting signal by ...heterologous protein to the ...as nuclear targeting signals, at least in the context of the full-length ...simian virus 40 (SV40) large T antigen, most of ... See full document
9
Roles of Us8A and Its Phosphorylation Mediated by Us3 in Herpes Simplex Virus 1 Pathogenesis
... The herpes simplex virus 1 (HSV-1) Us8A gene overlaps the gene that encodes glycoprotein E ...its phosphorylation at serine 61 (Ser-61), which we recently identi- fied as a ... See full document
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