[PDF] Top 20 Processing of the Epstein-Barr virus-encoded latent membrane protein p63/LMP.

... ment observed in the [3H]proline pulse-chase analysis, we performed immunoprecipitations with the S12 antibody on membrane and cytoplasmic fractions from the EBV+ BL line Jijoye and the [r] ... See full document

... Total membranes were solubilized in SDS, and immunoprecipitation was performed with the S12 monoclonal antibody + or an isotype-matched negative control -.. NP-40-soluble intracellular L[r] ... See full document

... The expression of LMP at high levels in lymphoid cells is cytotoxic and correlates with an increased expression of Bcl-2 following stable selection for the introduced LMP gene; 2 days af[r] ... See full document

... pstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus as- sociated with a wide variety of neoplasms, including Burkitt’s lymphoma (BL), nasopharyngeal carcinoma, posttransplant lym- phoproliferative ... See full document

... describe LMP-1’s negative regulation both of gene expression from EBV’s BamHI C (Bam C) and LMP-1 promoters and of its own stimulation of NF-␬B-dependent gene ...and LMP-1 promoters (14, 33, ...of ... See full document

... of Epstein-Barr virus (EBV) and its usual interaction with B lymphocytes is ...cases latent membrane protein 2A (LMP-2A), is expressed in this form of latency (7, 35, 36, ... See full document

... sites encoded in the Akata EBV strain genome and stably expressed in EBV-infected HK1 cells, shLMP1#1, was able to robustly knock down LMP1 expression under monolayer and ALI culture conditions ... See full document

... Histopathology analysis. Skin tumors and enlarged lymph nodes were harvested and fixed in buffered formalin. Tissues were embedded in paraffin blocks, sectioned, and stained with hematoxylin and eosin (H&E) by the ... See full document

... Crude membrane fractions were pre- pared from each tumor, and 100 m g of protein from each sample was subjected to SDS-PAGE with equal loadings dem- onstrated by Coomassie blue staining (data not ... See full document

... EBV LMP-1, LMP-2A and EA were retrieved from UniProKB Protein Database in Swiss Institute of Bioinformatics ...EBV LMP-1, LMP-2A and EA was analyzed by Protean module in DNAStar ...EBV ... See full document

... Another difference in our data is that coexistence of dual variants with and without 30-bp deletion in plasma but not NPC tissues from another group of patients. This dif- fers from other studies, whereby, existence of ... See full document

... 5-Azacytidine up regulates the expression of Epstein-Barr virus nuclear antigen 2 EBNA-2 through EBNA-6 and latent membrane protein in the Burkitt's lymphoma line Rael.. Activation of B-[r] ... See full document

... To our knowledge, the LMP1 gene might be the first viral oncogene identified as a p53 target. Interestingly, the LMP1 promoter region does not contain an obvious binding site for p53. Therefore, it is likely that p53 ... See full document

... In vitro evidence suggests that TRAF1 can affect LMP1 signaling by augmenting CTAR1-induced NF-␬B activation (7). In the present study, we have extended this finding and described a novel role for TRAF1 as a critical ... See full document

... and protein expression (expression of mRNA, translation of mRNA, expres- sion of DNA, translation of mRNA, translation of RNA, and expression of RNA) ...novel protein targets that contribute to the effects ... See full document

... detected virus, being found in ...EBV encoded LMP-1 protein in the tissue samples which removed from children aged between 2 and 12 years diagnosed with recurrent ...were LMP-1-positive ... See full document

... viral protein, a limited viral protein expression termed latency ...EBV latent genes—EBNA1, EBNA2, EBNA 3A, EBNA 3B, EBNA 3C, EBNA-LP, latent membrane protein 1 (LMP-1), ... See full document

... These experiments indicated that i at least 30% of the cytoplasmic LMP is in the plasma membrane, as opposed to other membrane compartments of the cell, ii the amino- terminal, carboxy-t[r] ... See full document

... Alteration of T-324 to a glycine residue had no detectable effect on the ability of LMP-1 to become serine phosphorylated or transform Rat-i cells.. Alteration of T-324 to a glutamate, h[r] ... See full document

... 10 min in 10 mM citrate buffer pH 6.0. They were then washed in phosphate buffered saline (PBS), covered by 5% bovine serum albumin (Sigma-Aldrich) for 30 min. Sections were treated with primary antibody for 2 h in a ... See full document