[PDF] Top 20 Over Production of Lactate in E.coli Engineered With an Acrylate Pathway
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Over Production of Lactate in E.coli Engineered With an Acrylate Pathway
... metabolically engineered lactic acid overproducers. This recombinant E. coli with a new acrylate pathway has great potential to meet the growing market demand of fermentation based ... See full document
8
High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway
... biosynthetic pathway in our ...violacein production from cheaper carbon sources is desirable from an economic ...for pathway engin- ...tion pathway in Escherichia coli, and achieved a ... See full document
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Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production
... synthesis pathway via the MVA pathway can be divided into three por- tions, referred to as “MvU” composed of acetyl-CoA acetyltransferase/3-hydroxy-3-methylglutaryl-CoA reductase (MvaE) and ... See full document
8
Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol
... an E. coli monoculture Several MA biosynthetic pathways have been assembled in heterologous microorganisms for de novo MA syn- thesis from simple carbon substrates [4, 17, ...this pathway [4, 20]. An ... See full document
10
Electrode-assisted acetoin production in a metabolically engineered Escherichia coli strain
... Interestingly, the deletion of frdA-D (fumarate reduc- tase), adhE (alcohol dehydrogenase), and ldhA (lactate dehydrogenase) led to a 1.25-fold increased growth rate under aerobic and decreased growth rates under ... See full document
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Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export
... Evolution has produced a huge variety of micro-organ- isms living in radically different environments. In particu- lar, some of these micro-organisms have evolved metabolic pathways leading to the synthesis of ... See full document
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Efficient Production of δ Guaiene, an Aroma Sesquiterpene Compound Accumulated in Agarwood, by Mevalonate Pathway Engineered Escherichia coli Cells
... MVA pathway enzymes, 3-hydroxy-3- methylglutaryl CoA (HMGCoA) synthase, HMG-CoA reductase, MVA kinase, phos- phomevalonate (PMVA) kinase, diphosphomevalonate (DPMVA) decarboxylase and type 2 isopentenyl ... See full document
11
Production of extracellular fatty acid using engineered Escherichia coli
... genetically engineered strains for fatty acid production only focused on the terminal pathway, such as the overexpression of the rate-limiting enzyme and removing feedback inhibi- tions in the fatty ... See full document
13
Deoxycytidine production by a metabolically engineered Escherichia coli strain
... EMP pathway, completely blocks isomerization of glucose 6-phosphate to fructose 6-phosphate, detouring the metabolism of glucose to the pentose phosphate (PP) ...PP pathway is to supply NADPH and ribose, ... See full document
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Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli
... and E. coli gldA in a strain defecient in lactate production and using an initial glucose concentration of ...Enhanced production of 1,2-propanediol in E. coli was also ... See full document
10
Efficient bio production of citramalate using an engineered Escherichia coli strain
... limitation, E. coli switches to fermentative metabolism, with wasteful formation of fermentation prod- ucts from ...Therefore, lactate dehydrogenase and pyruvate formate lyase were deleted to produce ... See full document
9
Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
... (MEP) pathway and the mevalonate (MVA) pathway (Figure 1) ...MEP pathway or MVA pathway to supply the intermediates DMAPP and IPP, they are unable to pro- duce the monoterpenes because of the ... See full document
10
MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli
... isopentenol production: The expression of codon-optimized nudF in E. coli W3110 led to the production of both isoprenol and prenol; overexpression of the HMBPP synthase gene ispG and DXP ... See full document
8
Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli
... The PCR fragments of gpps and ls were cloned into the BamHI–EcoRI and EcoRI–SalI sites of the pTrcHis2B vector to create p40Trc-ls-gpps and p40Trc-gpps-ls plas- mids. The generated ls-gpps and gpps-ls operons from ... See full document
10
An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli
... the production of new molecules to survival, we sought to drive the organism to produce new molecules or otherwise ...biosynthetic pathway and reconstructed a simple and reduced version incorporating only ... See full document
16
Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate
... (XI) pathway, ...from E. coli was introduced ...phosphate pathway (PPP) to increase the flux through the xylose metabolism ...[29] engineered a ...Weimberg pathway, which was ... See full document
17
Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli
... pHCA production of expressing in E. coli the genes encoding enzymes having PAL/TAL activities from ...strain production performance of the PTS + (W3110) and PTS − glucose + (VH33) phenotypes ... See full document
12
Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth
... Strains expressing different types of L-lactate dehydroge- nases had different growth rates when incubated at differ- ent temperatures, suggesting that L-lactate dehydrogenase activity may be the key ... See full document
11
Modular design of metabolic network for robust production of n-butanol from galactose–glucose mixtures
... we engineered robust Escherichia coli to accomplish high production of n-butanol from galactose–glucose mixtures via the design of modular pathway, an efficient and systematic way, to ... See full document
8
Direct conversion of theophylline to 3-methylxanthine by metabolically engineered E. coli
... biocatalytic production of several high value methylxanthines via metabolically engineered ...using E. coli engineered with ndmA and ... See full document
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