[PDF] Top 20 Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
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Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
... blood, collected in tubes containing EDTA or acid- citrate-dextrose, was centrifuged at 1,200 3 g for 10 ...clarified plasma was removed, aliquoted into sterile ...prepare dried plasma ... See full document
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Evaluation of Dried Blood Spots for Human Immunodeficiency Virus Type 1 Drug Resistance Testing
... blood spots (DBS) are simpler to prepare, store, and transport than plasma or serum and may represent a good alternative for drug resistance genotyping, particularly in resource-limited ...large ... See full document
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Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing
... HIV RNA cutoffs for virological failure are set, for example, 10,000 versus 1,000 copies/ml, as recommended by WHO in an ART strategy for low-income countries ...collect dried spots, store them at ... See full document
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Diminished Human Immunodeficiency Virus Type 1 DNA Yield from Dried Blood Spots after Storage in a Humid Incubator at 37°C Compared to −20°C
... on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other ...be ... See full document
5
Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... the quantification of human immunodeficiency virus type 1 (HIV-1) RNA from plasma were compared at three ...HIV-1-infected human ... See full document
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Detection and Quantification of Human Immunodeficiency Virus Type 1 p24 Antigen in Dried Whole Blood and Plasma on Filter Paper Stored under Various Conditions
... and quantification of HIV-1 in plasma were similar when samples were stored on filter paper and submitted to an HD p24 Ag assay and when they were stored frozen in polypropylene tubes ... See full document
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Comparative stabilities of quantitative human immunodeficiency virus RNA in plasma from samples collected in VACUTAINER CPT, VACUTAINER PPT, and standard VACUTAINER tubes
... HIV-1 RNA quantification by the bDNA assay. HIV type 1 (HIV-1) RNA was quantified by bDNA signal amplification-based hybridization (7, 10, 13, 14) using the Quantiplex HIV ... See full document
5
Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots
... of human immunodeficiency virus type 1 (HIV-1) drug resistance testing in resource-poor settings is still ...extracted from either whole blood or dried blood ... See full document
10
Performance of a Novel Human Immunodeficiency Virus (HIV) Type 1 Total Nucleic Acid Based Real Time PCR Assay Using Whole Blood and Dried Blood Spots for Diagnosis of HIV in Infants
... lection from infants by heel or finger stick is much easier to perform than liquid blood collection because it requires signif- icantly less phlebotomy ...HIV-1 RNA is detected together with proviral ... See full document
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Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... including human immunodeficiency virus (HIV) (2, 3–6, 7, 11, 12, 16, 17, ...19). Filter papers are especially useful for blood collection in resource-poor settings with limited access to ... See full document
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Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... man immunodeficiency virus (HIV)-infected patients ...HIV RNA in plasma, including (i) the Cobas TaqMan HIV type 1 (HIV-1) test (Roche Molecular Systems, Basel, Swit- ... See full document
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Use of Dried Spots of Whole Blood, Plasma, and Mother's Milk Collected on Filter Paper for Measurement of Human Immunodeficiency Virus Type 1 Burden
... viral RNA measurements is limiting and has mandated the search for suitable alternatives validated against this standard (18, ...Samples collected in this way may be subsequently transported to centralized ... See full document
6
Mapping and Characterization of the N-Terminal I Domain of Human Immunodeficiency Virus Type 1 Pr55Gag
... Data from the present study indicate that detergent resistance is mediated by the I ...units from subdomains other than the N-terminal I domain and the first zinc finger, must also be sufficient based upon ... See full document
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Pediatric Viral Human Immunodeficiency Virus Type 1 RNA Levels, Timing of Infection, and Disease Progression in African HIV-1-Infected Children
... HIV-1 RNA decrease over time may reflect a selection bias, considering the declining number of samples tested during ...specimens from healthier children may have been more often available for ... See full document
11
Detection of human immunodeficiency virus type 1 RNA in plasma samples from high risk pediatric patients by using the self sustained sequence replication reaction
... Detection of Human Immunodeficiency Virus Type 1 RNA in Plasma Samples from High-Risk Pediatric Patients by Using the Self-Sustained Sequence Replication Reaction.. There is an urgent ne[r] ... See full document
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Intra Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... plasma specimens. The laboratories performing the tests were chosen by the manufacturers. Enhanced-sensitivity bDNA as- says (ES-bDNA) were performed at the Chiron Reference Testing Laboratory (Emeryville, ... See full document
5
Stability of Human Immunodeficiency Virus Serological Markers in Samples Collected as HemaSpot and Whatman 903 Dried Blood Spots
... As DBS devices are more widely employed, the issues attendant to the variability of test results due to the inappropriate handling of specimens, contamination, or inade- quate laboratory quality control (26, 27) need to ... See full document
11
RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.
... C RNA in ...obtained from primer extension analyses in which the template consisted of either untreated C RNA (RNA only) or the same RNA following exposure to the chemical or enzyme ... See full document
9
Use of Dried Blood Spot to Improve the Diagnosis and Management of HIV in Resource Limited Settings
... Over 75% of people infected with HIV live in countries where health resources are very limited for the diagnosis and biological monitoring of people infected by the virus. In resource-limited settings, the use of ... See full document
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Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... The RNA concentrations measured by the standard and ultrasensitive methods differed by less than a factor of 2 for 91% (78 of 86) of the specimens that had RNA titers within the linear range of both ...The ... See full document
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