[PDF] Top 20 Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... usual isolates. Three reports describe real-time PCR assays for the detection of different Brucella ...HRM analysis or are able to identify novel strains (6, 9, ...300 ... See full document
6
Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... The use of several real-time PCR chemistries in one tube is made possible by the multichannel format of the Qiagen Rotor-Gene 6000 ...techniques, real-time PCR and HRM ... See full document
7
Rapid Identification of Bacterial Pathogens in Positive Blood Culture Bottles by Use of a Broad Based PCR Assay Coupled with High Resolution Melt Analysis
... our PCR-HRMA assay, and melting curve data generated from each of the samples were compared to our reference library for pathogen ...microbiologic analysis of these blood culture samples (reculturing and ... See full document
5
Real Time PCR and High Resolution Melt Analysis for Rapid Detection of Mycobacterium leprae Drug Resistance Mutations and Strain Types
... multiple isolates represented each of these ...the PCR products were sequenced, which confirmed the presence of the mixed alleles at codon 91 (see ... See full document
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Rapid Detection of Isoniazid Resistance in Mycobacterium tuberculosis Isolates by Use of Real Time PCR Based Melting Curve Analysis
... tuberculosis isolates. Further analysis of the frequencies of the mu- tation types according to their geographical locations revealed no difference, suggesting that the above mutation frequency would be ... See full document
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... the MAC and whether they harbored or lacked the insertion sequences IS901, IS1245, and IS900, according to methods described previously by Wilton and Cousins (22), Álvarez et al. (23), and Castellanos et al. (24), ... See full document
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Rapid Detection and Identification of Mucormycetes in Bronchoalveolar Lavage Samples from Immunocompromised Patients with Pulmonary Infiltrates by Use of High Resolution Melt Analysis
... RQ PCR assays. We designed new species-specific real-time PCR as- says (targeting the internal transcribed spacer 2 [ITS2] region of ribo- somal DNA) with Primer Express ...the PCR ... See full document
5
High Resolution Melting Curve Analysis for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis: a Meta Analysis
... tuberculosis isolates and used for real-time PCR in all ...perform real-time PCR and ...perform real-time PCR and ...two real-time PCRs ... See full document
6
A PCR–High Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
... the high rates of misidentification of NTHi as the closely related commensal ...molecular identification of NTHi is essential for surveillance of NTHi-targeted ...NTHi identification that can also ... See full document
5
Identification of Bacterial Pathogens in Ascitic Fluids from Patients with Suspected Spontaneous Bacterial Peritonitis by Use of Broad Range PCR (16S PCR) Coupled with High Resolution Melt Analysis
... (29). Identification of the causative agent in ascitic fluid after 16S rRNA gene amplification has relied on either probe-based amplicon analysis, which limits testing to a finite number of anticipated ... See full document
5
High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... the use of endpoint PCR and sequencing (11, 29) or four separate single-plex (12) or two separate duplex (30) real-time PCR ...allow discrimination between all four ... See full document
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... Therefore, rapid detection and accurate discrimination of Babesia ...is time consuming, has a low sensitivity and is unable to distinguish species, which ultimately requires a combined approach with ... See full document
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... any real-time PCR assay but are espe- cially critical when HRM analysis is ...for analysis with this assay (5). Currently, there exists no rapid and simple procedure which can ... See full document
7
Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... and identification of ...multiplex PCR, PCR restriction analysis, allele-specific PCR, and real-time PCR using fluorescent resonance energy transfer (FRET) probes ... See full document
5
Species Level Identification of Staphylococcal Isolates by Real Time PCR and Melt Curve Analysis
... A real-time PCR assay was developed to identify common staphylococcal ...unique melt properties of the probes resulting from sequence variations in the amplicons from each ...(CoNS) ... See full document
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Development and Evaluation of a Real Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei
... ical identification methods for culture isolates are problematic, especially rapid biochemical assays which have been shown to misidentify ...45). Rapid bacterial detection from clinical ... See full document
6
Rapid Identification of Measles Virus Vaccine Genotype by Real Time PCR
... In response to the need for prompt differentiation between vaccine reactions and wild-type measles virus infection cases, laboratories have been developing methods that do not require sequencing of N-450. A method ... See full document
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Rapid Determination of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosis by Real Time PCR
... rifampin-resistant isolates. A total of 44.7% of the rifampin-resistant isolates were susceptible to rifabutin, and ...the isolates with codon 531 and 513 mutations were resistant to all four rifamy- ... See full document
5
Fast and accurate identification of cryptic and sympatric mayfly species of the Baetis rhodani group
... In order to evaluate the diagnostic ability of the HRM curve method for the three species studied, we per- formed a principal component analysis (PCA) using the IBM SPSS v. 23 (IBM SPSS Statistics for Windows, ... See full document
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Rapid and Accurate Identification of Coagulase Negative Staphylococci by Real Time PCR
... Biprobe and primer design. The 16S rRNA gene sequences of 35 species of Staphylococcus, Stomatococcus mucilaginosus, and Micrococcus luteus were retrieved from the GenBank database. The sequences were aligned using the ... See full document
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