[PDF] Top 20 Rapid and Sensitive Detection of Mycobacterium ulcerans by Use of a Loop Mediated Isothermal Amplification Test
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Rapid and Sensitive Detection of Mycobacterium ulcerans by Use of a Loop Mediated Isothermal Amplification Test
... under isothermal conditions by relying on an en- zyme with strand displacement ...allow rapid detection using varied formats ...are rapid ( ⬃ 1 h) with sensitivity higher than or equal to that ... See full document
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Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... primers based on the unique, repetitive Neospora-specific Nc5 sequence which has not been detected in other taxa [7, 23]. We also checked the primer specificity against the GenBank database to ensure that non-specific ... See full document
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Sensitive and Rapid Detection of the New Delhi Metallo Beta Lactamase Gene by Loop Mediated Isothermal Amplification
... under isothermal con- ditions, LAMP is highly specific for the target sequence, and LAMP is simple and easy to perform), it shows a high rate of false-positive results in its current ...the amplification ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
... a loop-mediated isothermal amplification (LAMP) assay for rapid detection of ...of detection of 3 and 8 viral genome copies, ...diagnostic test results between the ... See full document
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Visual Detection of High Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
... simple, rapid, sensitive, qualitative, colorimetric loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye (HNB) was established to detect high-risk human ... See full document
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Original Article Rapid diagnosis of cisplatin-sensitive and resistant cervical squamous cell carcinomas by reverse transcription loop-mediated isothermal amplification
... its use is limited by the development of drug resistance, which is often rationalized via effects on cellular ...transcription loop-mediated isothermal amplification (RT-LAMP) analysis ... See full document
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Rapid, simple, and sensitive detection of the ompB gene of spotted fever group rickettsiae by loop mediated isothermal amplification
... The specificity of the LAMP assay was determined using a total of 42 strains of the order Rickettsiales (Table 1). These strains were provided by Dr Raoult D from the WHO Collaborating Centre for Rickettsial Reference ... See full document
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Rapid detection of the common avian leukosis virus subgroups by real time loop mediated isothermal amplification
... early detection and limi- tation of the virus carriers to prevent the vertical and horizontal ...specific, sensitive, inexpensive and real-time monitored LAMP assay for the detection of major ALV ... See full document
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Use of Loop Mediated Isothermal Amplification of the IS900 Sequence for Rapid Detection of Cultured Mycobacterium avium subsp paratuberculosis
... Primer design for LAMP. Four primers were used for LAMP reactions, two inner primers and two outer primers (Fig. 1). The two inner primers are called the forward inner primer (FIP) and the backward inner primer (BIP), ... See full document
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A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification
... years, isothermal amplifications, espe- cially loop-mediated isothermal amplification (LAMP) [12] has revolutionized the field of molecular diagnostics by offering rapid and ... See full document
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Rapid Detection of Candida auris Based on Loop Mediated Isothermal Amplification (LAMP)
... to use for DNA isolation, the swab was rubbed across the surface of an SDA plate, and this plate then was incubated at 37°C; the resulting small creamy colonies also were identified as ... See full document
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A simple and rapid method for detection of Goose Parvovirus in the field by loop mediated isothermal amplification
... of amplification can be accomplished with naked eye inspection through visual ...required use of gel electro- phoresis and staining with ethidium ...for amplification products could be per- formed by ... See full document
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Loop mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata
... the detection limit of the LAMP assay was 1 fg of ...the detection limit of the PCR is 100 pg, which shows that LAMP assay is more sensitive than PCR ...is sensitive and effective for ... See full document
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Rapid Detection of the Marek's Disease Viral Genome in Chicken Feathers by Loop Mediated Isothermal Amplification
... simple, rapid, and reliable methods for the diagnosis of serotype 1 MDV to prevent further disease transmission or ...the use of expensive equipment and costly con- ...under isothermal conditions and ... See full document
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Original Article Rapid identification of imipenem-resistant pseudomonas aeruginosa by loop-mediated isothermal amplification based detection of OprL and OprD2 genes
... The sensitivity of LAMP, which showed a detec- tion limit of 17.41 μg/L DNA, was 10-fold higher than that of conventional PCR. Several possible factors may contribute to this fact. LAMP is inherently tolerant of ... See full document
8
Rapid detection of benzimidazole resistance in Botrytis cinerea by loop-mediated isothermal amplification
... a loop-mediated isothermal amplification (LAMP) method was established for rapid detection of benzimidazole resistance in ...no amplification was detected with other ... See full document
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Loop Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species
... acid amplification method termed loop-mediated isothermal amplification (LAMP) has been de- veloped ...under isothermal con- ditions. Since LAMP is done under isothermal ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus
... DNA amplification as- ...the use of unprocessed samples (versus what was found for extracted DNA samples), the assay could still be very useful in clinical ... See full document
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Sensitive and Rapid Detection of Herpes Simplex Virus and Varicella Zoster Virus DNA by Loop Mediated Isothermal Amplification
... each deoxynucleoside triphosphate, a 500 nM concentration of each primer, and 0.5 unit of Taq DNA polymerase. Initial denaturation was done at 95°C for 3 min, followed by 40 cycles of denaturation (30 s at 95°C), ... See full document
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Specific, sensitive and rapid detection of human plasmodium knowlesi infection by loop mediated isothermal amplification (LAMP) in blood samples
... There are however, limitations to this method. For visualization of results under UV light, the product tubes need to be opened for the dye to be added and this might lead to cross-contamination. This limitation can be ... See full document
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