[PDF] Top 20 Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers
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Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers
... the V3 tip shields the remainder of ...the antigenicity and low- resolution EM data together show that no such destabilization ...the SOSIP.664-E64K.M1M7 trimers are fully ... See full document
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Differential binding of neutralizing and non neutralizing antibodies to native like soluble HIV 1 Env trimers, uncleaved Env proteins, and monomeric subunits
... the SOSIP modifications were present in the uncleaved gp140, ...when Env is ...the native-like SOSIP.R6 trimers and the other Env variants were even starker than for ...different ... See full document
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A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene
... the HIV-1 ...For HIV-1, one approach involves using recombinant, soluble protein mimics of the native ...make native-like, soluble trimers in ... See full document
16
Influences on the Design and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers
... nonnative trimers. EM images show that the latter are not true trimers but are oligomeric proteins that contain three splayed-out gp120 subunits linked to a central gp41 ECTO core via the uncleaved ... See full document
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Immunogenicity in Rabbits of HIV-1 SOSIP Trimers from Clades A, B, and C, Given Individually, Sequentially, or in Combination
... Recombinant soluble HIV-1 envelope glycoprotein (Env) SOSIP trimers are a design platform for inducing broadly neutralizing antibodies (bNAbs) by vacci- ...of ... See full document
15
Effects of Adjuvants on HIV-1 Envelope Glycoprotein SOSIP Trimers In Vitro
... ABSTRACT Native-like, soluble, recombinant SOSIP trimers of various designs and based on several env genes of human immunodeficiency virus type 1 (HIV-1) are ... See full document
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Improving the Expression and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers by Targeted Sequence Changes
... high-resolution Env structures, including those of BG505 SOSIP.664 trimers in complex with PGT145, are now available, the mechanism underlying the subtle breathing of the trimer apex remains to be ... See full document
23
Changes in Structure and Antigenicity of HIV-1 Env Trimers Resulting from Removal of a Conserved CD4 Binding Site-Proximal Glycan
... for HIV-1 broadly neutralizing antibodies ...that HIV has evolved to escape the host’s immune response is to mask conserved epitopes on Env with dense ...ing native-like ... See full document
13
Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers
... Dispersed Env elements increase stability of the NFL trimers. The BG505 Env-derived NFL glycoprotein readily forms trimers that adopt a native-like ...These trimers can be ... See full document
12
Epitope-Independent Purification of Native-Like Envelope Trimers from Diverse HIV-1 Isolates
... variant-specific Env structural features, which may underlie differences in antigenicity and ...of Env SOSIP trimers demonstrated that while our purified trimers retain the ... See full document
12
Influences on Trimerization and Aggregation of Soluble, Cleaved HIV-1 SOSIP Envelope Glycoprotein
... desired trimers can be purified and are stable in the absence of ...resulting SOSIP.664 gp140 trimers are more suitable than their predeces- sors for structural and, perhaps, immunogenicity ... See full document
13
Structural Characterization of Cleaved, Soluble HIV-1 Envelope Glycoprotein Trimers
... the SOSIP trimer as it is soluble, does not aggregate, and is easier to express and ...the SOSIP trimer is ideally suited for further structural investigation of bNAb com- ...the HIV-1 ... See full document
8
Structure-Based Design of a Soluble Prefusion-Closed HIV-1 Env Trimer with Reduced CD4 Affinity and Improved Immunogenicity
... neutralizing V3 antigenicity, we found further stabilization of the DS-SOSIP variant of the HIV-1 Env trimer in the prefusion-closed conformation to reduce the affinity to CD4 to ... See full document
18
Murine Antibody Responses to Cleaved Soluble HIV-1 Envelope Trimers Are Highly Restricted in Specificity
... BG505 SOSIP.664 trimer immunogens because they are more native-like in conformation (27–29) than previous versions of Env ...more native-like epitopes on BG505 SOSIP.664 ... See full document
16
Native Conformation and Canonical Disulfide Bond Formation Are Interlinked Properties of HIV-1 Env Glycoproteins
... successful HIV-1 vaccine will include a recombinant form of the Env protein, a trimer located on the virion ...purification, Env proteins are often made in truncated, soluble ...the ... See full document
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High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers
... an adjacent monomer only when PGT151 is bound between the two monomers (Fig. 5C). We hypothesized that replacing threonine with the larger phenylalanine moiety produced more extensive contacts between gp41 monomers and ... See full document
18
Single-Chain Soluble BG505.SOSIP gp140 Trimers as Structural and Antigenic Mimics of Mature Closed HIV-1 Env
... trimeric HIV-1 envelope glycoprotein (Env) is the sole target of virus-directed neutralizing antibody responses and a pri- mary focus of vaccine ...design. Soluble mimics of Env have ... See full document
12
Presenting native like HIV 1 envelope trimers on ferritin nanoparticles improves their immunogenicity
... neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope ...potent HIV-1 neutraliza- tion by a human antibody that binds the gp41-gp120 ... See full document
5
Antigenic and 3D structural characterization of soluble X4 and hybrid X4 R5 HIV 1 Env trimers
... Results: Soluble trimeric gp140 Env constructs were used as immunogenic mimics of the native spikes to analyze their antigenic properties in the context of their overall 3D ...generated ... See full document
12
Enzyme Digests Eliminate Nonfunctional Env from HIV-1 Particle Surfaces, Leaving Native Env Trimers Intact and Viral Infectivity Unaffected
... the HIV Env VLPs was preserved in the face of ...other Env VLPs were preserved after ...(VSV-G) Env VLP infectivity was also reduced to back- ground ...of native HIV-1 ... See full document
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