[PDF] Top 20 Is the 135S Poliovirus Particle an Intermediate during Cell Entry?
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Is the 135S Poliovirus Particle an Intermediate during Cell Entry?
... the cell surface induces a conformational transition which generates an altered particle with a sedimentation value of 135S versus the 160S of the native ...the 135S particle is a ... See full document
5
The Structure of the Poliovirus 135S Cell Entry Intermediate at 10-Angstrom Resolution Reveals the Location of an Externalized Polypeptide That Binds to Membranes
... the 135S-135S(V8) difference map ...the 135S particle that is absent in reconstructions of the V8- cleaved particle ...V8-cleaved 135S. (Lane 1) The 135S particle ... See full document
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Molecular Tectonic Model of Virus Structural Transitions: the Putative Cell Entry States of Poliovirus
... picornavirus cell entry is known only in outline (14, 48, 49, 51–53, ...the 135S, or A, particle. The 135S particle has a lower sedimentation coef- ficient (135S versus ... See full document
13
Characterization of Early Steps in the Poliovirus Infection Process: Receptor-Decorated Liposomes Induce Conversion of the Virus to Membrane-Anchored Entry-Intermediate Particles
... beled poliovirus was mixed with liposomes for 5 min at room temperature, and virus binding was measured as the extent of colocalization of radioactivity and liposomes by lipid flotation ...to cell ... See full document
9
The Poliovirus Empty Capsid Specifically Recognizes the Poliovirus Receptor and Undergoes Some, but Not All, of the Transitions Associated with Cell Entry
... the cell entry ...the cell entry intermediate does not rely on the rearrangements which occur in the inner surface of the capsid during the final stages of mature virus ... See full document
6
Morphological Changes in the T=3 Capsid of Flock House Virus during Cell Entry
... In this study we have investigated early cell entry events associated with FHV infection of Drosophila cells. We ob- served that nearly all input virus bound to cells at a low tem- perature, whereas a ... See full document
8
Dissecting the Cell Entry Pathway of Baculovirus by Single-Particle Tracking and Quantitative Electron Microscopic Analysis
... AcMNPV entry into Sf9 cells. After prebinding to the cell surface at 4°C, AcMNPV virus particles were incubated with cells for 1 ...the cell surface or in ...virus particle bound to the ... See full document
25
The poliovirus 135S particle is infectious.
... the 135S particle is a necessary intermediate in the process of ...eluted 135S particles accumulate early in infected cells (18, ...the 135S form by binding to and stabi- lizing the ... See full document
7
Nectin-Like Interactions between Poliovirus and Its Receptor Trigger Conformational Changes Associated with Cell Entry
... More recently, two high-resolution crystal structures were published that included glycosylated versions of domain 1 (D1) of Pvr. One structure shows the binding of D1 to the immune regu- lator TIGIT (40). The other is a ... See full document
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RNA Transfer from Poliovirus 135S Particles across Membranes Is Mediated by Long Umbilical Connectors
... a 135S-like intermediate should allow additional intermolecular contacts to form that reduce the flexi- bility of the tethers and rotate the virus so that a randomly chosen quasi-3-fold axis is brought into ... See full document
12
Structure and Function of a Genetically Engineered Mimic of a Nonenveloped Virus Entry Intermediate
... unit cell and one particle in each crystallographic asymmetric unit, defining a 60-fold noncrystallographic symmetry ...unit cell was further refined using a 1-dimensional (1D) locked rotation ... See full document
10
The μ1 72-96 Loop Controls Conformational Transitions during Reovirus Cell Entry
... Additionally, during cell entry, 1 undergoes structural rearrangements that facilitate deliv- ery of the viral cores across the ...for cell entry, we characterized viruses with ... See full document
11
Managing the risk of circulating vaccine derived poliovirus during the endgame: oral poliovirus vaccine needs
... Table 3 shows the model estimates of total expected tOPV and bOPV vaccine needs from RI and SIAs com- bined to manage cVDPV risks during the OPV cessa- tion period. The global RI vaccine needs of approximately 620 ... See full document
11
Single-Particle Detection of Transcription following Rotavirus Entry
... each particle, as well as the maximum projection of the overlay of all three ...The particle highlighted in the green box in panel A and shown in B is represented here as a green circle plotted within the ... See full document
18
Interaction of Translation Initiation Factor eIF4B with the Poliovirus Internal Ribosome Entry Site
... on poliovirus translation, the binding of eIF4B appears not to be affected by ...the poliovirus IRES ...the poliovirus IRES was slightly weaker (lane ... See full document
10
Dual Stem Loops within the Poliovirus Internal Ribosomal Entry Site Control Neurovirulence
... to poliovirus (PV) infection is largely confined to a specific subpopulation of neuronal ...by cell-external components such as the PV receptor CD155, as well as cell-internal constraints such as the ... See full document
7
Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology
... The third unique feature of annual killifish develop- ment is the ability to enter diapause. Diapause is a state of dormancy that retards or blocks embryonic develop- ment in anticipation of predictable cyclic hostile ... See full document
22
Inhibition of host cell transcription by poliovirus: cleavage of transcription factor CREB by poliovirus-encoded protease 3Cpro.
... cleaved during infection of HeLa cells with poliovirus, cell extracts were prepared from mock- and polio- virus-infected cells and examined by Western blot analysis us- ing a mouse monoclonal ... See full document
7
Cell type specific requirements for thiol/disulfide exchange during HIV 1 entry and infection
... the entry of HIV-1 in resting T cells, and the establishment of virus ...Trx during HIV-1 entry (Figures 3D,E and 5), the observed elevation of plasma Trx during chronic stages of HIV disease ... See full document
15
Effect of mutations downstream of the internal ribosome entry site on initiation of poliovirus protein synthesis.
... 68, 1994 DISCUSSION The initiation codon of the poliovirus polyprotein at AUG743 is separated from the 3' border of the IRES by a 154 nt spacer which may contain cis-acting elements that[r] ... See full document
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