[PDF] Top 20 Sequencing Assays for Failed Genotyping with the Versant Hepatitis C Virus Genotype Assay (LiPA), Version 2 0

... fied in the NS5B region in this study (74.5%) was lower than the values normally observed in our laboratory (90% with HC3/HC5 primers during the same period of time) or described in other studies (82 to 97.3%) (10, 25). ... See full document

... HCV genotype 2 (14). This is particularly noteworthy, as genotype misclassification may lead to suboptimal treatment of patients infected with these recombinant strains, particularly where no ... See full document

... duration. Assays that are recommended for genotyping are those that can discrim- inate genotyping/subtyping with amplification of the 5’UTR region plus one other region, usually the NS5B or core ... See full document

... type 2 (40/312), ...and 2%, respectively, that has been reported previously ...HCV genotype 4 in our population doubles with the inclusion of mixed-genotype ...HCV genotype 4 could be ... See full document

... DNA sequencing of the partial C/E1 PCR products from a cohort of Italian dialysis patients confirmed the accuracy of HTA genotype assignment in all 15 individuals but failed to identify type ... See full document

... containing genotype 3b, initial Invader ...yielded genotype 3 and 4 assignments fol- lowing a 30-min incubation and genotype 3 assignments (both replicates) after a 60-min ...for assay F, ... See full document

... the assay for HCV genotypes 2 and 3, whereas the concordance of the results with those of the geno- typing assay in identifying these types remained ...serotyping assays in the diagnosis of ... See full document

... Hepatitis C virus genotyping assays are usually based on the sequence analysis of an amplified segment of the genome, which is commonly the 5’ untranslated ...based assays are ... See full document

... Hepatitis C viruses (HCVs) display a high level of sequence diversity and are currently divided into six ...probe assay (LiPA), which targets the 5 ⴕ untranslated region (5 ⴕ UTR) of the HCV ... See full document

... for 2 min on an Applied Biosystems thermal cycler model GeneAmp PCR System ...HCV genotyping results obtained with the NS5b sequencing method, the Versant HCV Genotype assay, and ... See full document

... HCV genotyping. All 63 samples were subjected to clonal sequencing of the NS5B sequence (222 bp, positions 8316 to 8537) as the reference method ...myeloblastosis virus (AMV) reverse transcriptase ... See full document

... both assays. The NS5b sequencing results for these samples showed that Versant HCV genotype assay (LiPA) ...HCV genotype and subtype and thereby showed an improvement in ... See full document

... both assays. Patients infected with genotypes 2 and 4 showed similar results, but the number of samples with these genotypes was too low for ...quantitation assays were in- dependent of the genotypes ... See full document

... HCV genotype can predict response to interferon or combi- nation therapy in patients with chronic hepatitis C (3, 4, 7, 17, 18, 19, ...HCV genotype has been implicated as a predictive factor ... See full document

... available assays for the quantification of ...1.0 assay (Chiron Corporation, Emeryville, ...quantitative assays made the task of accurately assessing the influence of HCV viral load and ... See full document

... leukemia virus reverse transcriptase and T7 RNA polymerase in HEPES-Tris buffer) was added to each tube and the mixture was incubated at ...protection assay, 100 ␮l of HCV probe reagent (chemiluminescent ... See full document

... (genotype 2) and 11 genotype (4), geno- typing was based on core region only (Table ...as genotype 4 while the NS5B was identified as genotype 1 indi- cating the presence of putative ... See full document

... position 2 99, respectively (12, 14). The current assay is improved by including the T-C polymorphism at position 2 ...at 2 99 were found (12, ...position 2 99 for the type 1 ... See full document

... AMPLICOR assay. This and other qualitative, nonquantitative assays can detect the presence of HCV RNA, but they cannot measure HCV viral ...commercial assays, namely, the noncompetitive PCR-based ... See full document

... HCV genotype 4 referred to our Hepatology Reference Center with a diagnosis of chronic HCV infection and tested in our labo- ratory or diagnosed with HCV infection at the Cerba ...HCV genotype 4 infection, ... See full document