[PDF] Top 20 Herpes Simplex Virus 1 Protein UL37 Interacts with Viral Glycoprotein gK and Membrane Protein UL20 and Functions in Cytoplasmic Virion Envelopment
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Herpes Simplex Virus 1 Protein UL37 Interacts with Viral Glycoprotein gK and Membrane Protein UL20 and Functions in Cytoplasmic Virion Envelopment
... that glycoprotein K (gK) and its interacting partner, the UL20 protein, play crucial roles in virion ...either gK or UL20 fail to acquire an envelope, thus causing ... See full document
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The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment
... HSV-1 UL20 Previously, we reported on the construction and charac- terization of a panel of 31 mutations within the UL20 gene ...included: 1) cluster-to-alanine mutants in which a cluster of ... See full document
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UL20 Protein Functions Precede and Are Required for the UL11 Functions of Herpes Simplex Virus Type 1 Cytoplasmic Virion Envelopment
... and UL20 genes in cytoplasmic envelopment, we constructed the ⌬UL20/UL11 double-null ...double-null virus exhibited replication characteristics very similar to those of the ... See full document
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Phenylalanine Residues at the Carboxyl Terminus of the Herpes Simplex Virus 1 UL20 Membrane Protein Regulate Cytoplasmic Virion Envelopment and Infectious Virus Production
... for 1 h with 2% fetal calf serum in ...diluted 1:200 in PBS blocking buffer, followed by Alexa Fluor 555- conjugated goat anti-mouse IgG1 and Alexa Fluor 488-conjugated goat anti-mouse IgG2a secondary ... See full document
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Genetic Analysis of the Herpes Simplex Virus Type 1 UL20 Protein Domains Involved in Cytoplasmic Virion Envelopment and Virus-Induced Cell Fusion
... and membrane topol- ogy of ...infectious virus production and virus-induced cell ...in protein-protein interactions necessary for UL20p-associated viral ...the UL20-null ... See full document
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Herpes Simplex Virus 1 UL37 Protein Tyrosine Residues Conserved among All Alphaherpesviruses Are Required for Interactions with Glycoprotein K, Cytoplasmic Virion Envelopment, and Infectious Virus Production
... The herpes simplex virus 1 (HSV-1) UL37 protein functions in virion envelopment at trans-Golgi membranes, as well as in retro- grade and anterograde ... See full document
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Functional and Physical Interactions of the Herpes Simplex Virus Type 1 UL20 Membrane Protein with Glycoprotein K
... fusion protein did not contain the 3 ⫻ FLAG epitope; therefore, any potential inter- action of the 3 ⫻ FLAG epitope with the ProtC epitope could not be responsible for the observed physical interaction be- tween ... See full document
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The Herpes Simplex Virus Type 1 UL20 Protein and the Amino Terminus of Glycoprotein K (gK) Physically Interact with gB
... utilizes membrane fusion for virus entry into cells as well as for spreading from infected to uninfected ...These membrane fusion events are thought to be medi- ated by the concerted action of ... See full document
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The Herpes Simplex Virus Type 1 UL20 Protein Modulates Membrane Fusion Events during Cytoplasmic Virion Morphogenesis and Virus-Induced Cell Fusion
... the UL20 protein is required in transient expression assays for the Golgi- dependent glycosylation and cell surface expression of gK, implying a functional association between the UL20 ... See full document
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Functional Hierarchy of Herpes Simplex Virus 1 Viral Glycoproteins in Cytoplasmic Virion Envelopment and Egress
... the membrane-associated protein UL11 (18, 30, 32, 36, 43, 48, 57) ...of viral plaques pro- duced (5) and an increase in the accumulation of unenveloped capsids in the cytoplasm of ... See full document
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Plasma Membrane Topology of Syncytial Domains of Herpes Simplex Virus Type 1 Glycoprotein K (gK): the UL20 Protein Enables Cell Surface Localization of gK but Not gK-Mediated Cell-to-Cell Fusion
... of gK sug- gested the presence of four membrane-spanning domains ...vitro-translated gK, which con- tained truncations, as well as in-frame deletions of putative transmembrane regions, showed that ... See full document
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The Herpes Simplex Virus 1 UL34 Protein Interacts with a Cytoplasmic Dynein Intermediate Chain and Targets Nuclear Membrane
... the herpes simplex virus 1 capsid-tegument structure released by deenvelopment during entry into cells must be transported retrograde to the nuclear pore where viral DNA is released ... See full document
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The Herpes Simplex Virus 1 UL51 Protein Interacts with the UL7 Protein and Plays a Role in Its Recruitment into the Virion
... deletion virus. In fractions from wild-type virus-infected cells, pUL7 cosedimented with pUL51 and was abundant in the virion ...the virion fractions was greatly re- duced in the UL51 deletion ... See full document
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Herpes Simplex Virus Type 1 Glycoprotein K and the UL20 Protein Are Interdependent for Intracellular Trafficking and trans-Golgi Network Localization
... Final envelopment of the cytoplasmic herpes simplex virus type 1 (HSV-1) nucleocapsid is thought to occur by budding into trans-Golgi network (TGN)-derived ...highly ... See full document
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The Capsid Protein Encoded by UL17 of Herpes Simplex Virus 1 Interacts with Tegument Protein VP13/14
... 17 protein (pU L 17) of herpes simplex virus 1 (HSV-1) likely associates with the surfaces of DNA- containing capsids in a heterodimer with pU L ...with viral light ... See full document
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Direct and Specific Binding of the UL16 Tegument Protein of Herpes Simplex Virus to the Cytoplasmic Tail of Glycoprotein E
... the virion, the UL16- interaction network must interface in some manner with viral glycoproteins on the ...the membrane is via UL11; however, because that protein does not span the ... See full document
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The Herpes Simplex Virus Type 1 Glycoprotein D (gD) Cytoplasmic Terminus and Full-Length gE Are Not Essential and Do Not Function in a Redundant Manner for Cytoplasmic Virion Envelopment and Egress
... in virus replication and ...in cytoplasmic virion ...interactive protein complex “fusion machine” that in- volves complex interactions among gD, gB, and gH and, pos- sibly, other viral ... See full document
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Nucleolin Interacts with US11 Protein of Herpes Simplex Virus 1 and Is Involved in Its Trafficking
... Herpes simplex virus type 1 (HSV-1) infection induces profound nucleolar modifications at the functional and organiza- tional levels, including nucleolar invasion by several ... See full document
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Herpes Simplex Virus Type 1 Cytoplasmic Envelopment Requires Functional Vps4
... on viral genome and protein ...ated membrane budding, the inhibition of HSV-1 replication could potentially be due to secondary effects on other aspects of the virus life cycle, rather ... See full document
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Membrane Association of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... of viral proteins, including tegument proteins, glycoproteins, and as- sembled nucleocapsids, converge at cytoplasmic vesicles to be- come incorporated into ...of virion assembly (43, 59). Therefore, ... See full document
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