[PDF] Top 20 Single Cell Gel Electrophoresis (Comet Assay) Mediated Evaluation of DNA Damage in Buccal Epithelial Cells Among Alcohol Users
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Single Cell Gel Electrophoresis (Comet Assay) Mediated Evaluation of DNA Damage in Buccal Epithelial Cells Among Alcohol Users
... Buccal epithelial cells were collected from subjects by using a soft bristle toothbrush gently from the oral ...the buccal cells and incubated for 30min. at 37°C. The cells were ... See full document
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Evaluation of the Genotoxicity of Chlorpyrifos in Common Indus Valley Toad, Bufo stomaticus Using Alkaline Single Cell Gel Electrophoresis (Comet) Assay
... the evaluation of DNA strand breakage, we selected to employ alkaline single-cell gel electrophoresis/comet assay to quantify DNA damage in the ... See full document
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THE ANTINEOPLASTIC ACTIVITY OF ECHITAMIDINE N OXIDEGLUCOPYRANOSIDE ISOLATED FROM STEM BARK EXTRACT OF ALSTONIA SCHOLARIS IN KB CELLS
... of DNA damage by EOG was further confirmed by comet assay or single cell gel ...The single cell gel electrophoresis assay developed by ... See full document
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LYMPHOCYTES DNA DAMAGE FROM PANMASALA, GUTKHA, KHARRAH, CHEWING TOBACCO USERS AND CHAIN SMOKERS OF CENTRAL INDIA, BY USING SINGLE-CELL GEL ELECTROPHORESIS ASSAY
... consumption. Single-cell gel electrophoresis assay or comet assay is a sensitive and rapid method for DNA strand breaks; it further provides information on amount ... See full document
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Evaluation of genotoxicity by comet assay (single-cell gel electrophoresis) in tissues of the fish Cyprinus carpio during sub-lethal exposure to Karanjin
... the comet assay which is an important biomarker of genotoxicity in fish (Mitchelmore & Chipman, ...1998). Comet assay is widely used, simple, and sensitive technique for evaluating in ... See full document
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Evaluation of Buffalo Bull Spermatozoal Dna Damage Using Single Cell Gel Electrophoresis
... sperm DNA integrity. The cryopreservation promotes DNA fragmentation in ...detectable DNA damage was positively correlated with the cryo- damaged cells in each treatment ( P ...sperm ... See full document
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Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
... value. Among the habitat drivers, temperature related effects on the genome were considered in this ...of single cells were assessed by Comet ...stages. Single Cell Gel ... See full document
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Evaluation of DNA Damage by Methyl Methane Sulfonate in Allium cepa Root Cells by Comet Assay
... root cells using a mild alkaline comet assay (Single Cell Gel ...Electrophoresis). Single Cell Gel Electrophoresis has recently been applied to ... See full document
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Assessment of DNA damage in pulmonary tuberculosis patients by single cell gel electrophoresis / comet assay
... the DNA and this damage may be induced by free radicals generated by this drug 9 ...reliable comet measurements that would truly reflect the extent of DNA damage induced by the ... See full document
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DNA damage and cell death assessment in patients with severe multiple trauma using comet assay
... stress-induced cell death by apoptosis, but its exact mechanisms is not fully understood ...[8-12]. DNA damage including oxidative DNA bases modifica- tion is one of the intrinsic signals ... See full document
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NATURAL ALTERNATIVES TO TREAT CANCER: A STUDY ON ANTICANCER ACTIVITY OF LAWSONIA INERMIS LINN
... Analysis: DNA fragmentation is the biochemical hallmark of ...Molt-4 cells and human lymphocytes were exposed to nisin at the cytotoxic concentration, and the cells were incubated at 37 ºC for 6 h as ... See full document
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Evaluation Of Genetic Damage In Buccal Epithelial Cells Of Asbestos Industry Workers Using Micronuclues Assay
... exfoliated cells in epithelial tissue have been used to evaluate the genotoxic ...micronucleus assay can be used to show both clastogenic and aneugenic ... See full document
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Impact of tire debris on in vitro and in vivo systems
... A549 cell line and X. laevis embryos to TD extracts. A549 cell proliferation decreased in a time- and dose-dependent manner, and a significant statistical decrease appeared at 24 h and became more evident ... See full document
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Optimization of Alkaline Single Cell Gel Electropohoresis (Comet Assay) Protocol for Cells from Oral Cavity
... with cells in suspension, while the volume of proteinase K digestion was increased from 100 (protocol 3) to 500 μl (protocol ...When cells were digested in suspension at the same temperature and time as ... See full document
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Hepatoprotective and antiapoptotic role of aged black garlic against hepatotoxicity induced by cyclophosphamide
... from DNA damage investigated by comet assay revealed that CP administra- tion caused marked degree of DNA damage as there was a significant increase number (P < ...in ... See full document
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DNA DAMAGE ANALYSIS IN MAMMALIAN CELLS BY SMA-DMSO COMPLEX USING COMET ASSAY
... mutation assay and Chromosomal Aberration ...CHO cells under experimental conditions, whereas, the above concentrations (1M and 10M) were ...pharmaceuticals.Comet Assay was conducted in current work ... See full document
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The prognostic value of DNA damage level in peripheral blood lymphocytes of chemotherapy-naïve patients with germ cell cancer
... limited DNA repair may be responsible for unique curability of this type of cancer [6, ...increasing DNA repair capacity, since the data reported so far are rather inconclusive [7, 8, ...from DNA ... See full document
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CELL VIABILITY AND CYTOTOXIC EFFECT OF ACANTHOPHORA SPICIFERA (RED SEAWEED) ON HT29 COLON CANCER CELL LINE
... of cell viability after the treatment of methanolic extract of Acanthophora spicifera (10-200 ...In DNA Fragmentation by comet assay showed the methanolic extract of Acanthophora spicifera, ... See full document
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Apoptosis induction in human leukemia cells by novel 2-amino-5-benzylthiazole derivatives
... particularly DNa damage in human leukemia cells, by the novel synthesized thiazole derivatives ‒ 2,8-dimethyl-7-(3-trifluo - romethyl-benzyl)pyrazolo[4,3-e]thiazolo[3,2-a]pyrimidin-4(2H)-one ... See full document
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Alkaline single cell gel electrophoresis: A rapid technique to evaluate DNA damage in type 2 diabetes mellitus patients
... the comet assay to measure the extent of DNA strand-break damage induced by T2DM, as the technique is mostly reliable and less susceptible to ...of damage (Collins et ...of DNA ... See full document
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