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SP01 gene 27 is required for viral late transcription.
... RNA labeled at the indicated late times after infection with wildtype SPOt or with various DO mutants of SPOt was hybridized to nitrocellulose-bound EcoRI fragments of SPOt DNA.. Cell eq[r] ... See full document
6
Murine Cytomegalovirus Protein pM92 Is a Conserved Regulator of Viral Late Gene Expression
... the viral late transcription regulators, which includes recently re- ported pM79 ...in viral late transcription, we examined transcript accumula- tion of multiple early genes ... See full document
12
Murine Cytomegalovirus Protein pM79 Is a Key Regulator for Viral Late Transcription
... at late times is poorly ...(CMV) gene, UL79, is required for late gene ...nuclear viral replication compart- ments. M79 transcription was significantly decreased in the ... See full document
13
Analysis of an Autographa californica Multicapsid Nucleopolyhedrovirus lef-6-Null Virus: LEF-6 Is Not Essential for Viral Replication but Appears To Accelerate Late Gene Transcription
... lef-6 gene was not essential for virus replication in Sf9 ...not required for either viral DNA replication or late tran- ...However, late transcription was substantially delayed ... See full document
12
The Adenovirus L4 33-Kilodalton Protein Binds to Intragenic Sequences of the Major Late Promoter Required for Late Phase-Specific Stimulation of Transcription
... the late phase. In contrast, the proposition that a late protein encoded within the ML transcription unit participates in ML transcription appears paradoxical: the L4 33-kDa protein can- not ... See full document
12
Viral DNA Replication Orientation and hnRNPs Regulate Transcription of the Human Papillomavirus 18 Late Promoter
... two viral replication proteins, E1 and E2 ...is required for viral DNA replication initiation and is also necessary throughout the replication elongation process (29, ...not required during ... See full document
21
Viral Late Domains
... (83), gene transcription (32, 73), and regulation of the ubiquitination and stability of the Ub ligase MDM2 and the tumor suppressor p53 ...are required for interaction with other subunits of the ... See full document
9
Conditional repression of the E2 transcription unit in E1-E3-deleted adenovirus vectors is correlated with a strong reduction in viral DNA replication and late gene expression in vitro.
... layers were infected with defined volumes of virus preparations. The cells were fixed 16 h after infection before the onset of a second round of infection and stained with a monoclonal antibody specific for DBP (B6a72K ... See full document
5
The Human Cytomegalovirus Gene UL79 Is Required for the Accumulation of Late Viral Transcripts
... of viral late proteins (pp71 and pp28) during infection ...of viral factors in HCMV ...is required for the accumulation of late viral transcripts, it is possible that it is a ... See full document
12
A cis Repression Sequence Adjacent to the Transcription Start Site of the Human Cytomegalovirus US3 Gene Is Required To Down Regulate Gene Expression at Early and Late Times after Infection
... the viral IE72 and IE86 proteins are not fully under- ...and viral promoters, and has a sig- nificant synergistic effect on IE86-mediated activation of early viral promoters (8, 18, 20, 30, ... See full document
10
Human foamy virus reverse transcription that occurs late in the viral replication cycle.
... was required for its ...env gene-negative mutant may either indi- cate a physiological phenomenon or represent an artifact due to the assay ...marker gene (data not ... See full document
7
Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts
... and late gene transcription of DNA viruses, IE proteins recruit the general transcription factors, including TBP, to the ...general transcription factors, recognition of the TATA box in ... See full document
9
The vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression.
... of transcription. The components required for early gene transcription in vitro have been extracted from vaccinia virions (16, 40); they consist of the multisubunit RNA polymerase (4, 47), the ... See full document
7
The Human Cytomegalovirus Gene Products Essential for Late Viral Gene Expression Assemble into Prereplication Complexes before Viral DNA Replication
... for transcription of the UL44, UL75 (gH), and UL99 (pp28) late ...-95 viral proteins are expressed before viral DNA replication and assemble at the viral ...for viral gene ... See full document
16
Interaction between ORF24 and ORF34 in the Kaposi's Sarcoma-Associated Herpesvirus Late Gene Transcription Factor Complex Is Essential for Viral Late Gene Expression
... FIG 4 The ORF24-ORF34 interaction is required for activation of a late gene promoter and progeny virion production. (A) Cells were transfected with all six WT vTFs or the empty vector and assessed ... See full document
6
The Epstein-Barr Virus BDLF4 Gene Is Required for Efficient Expression of Viral Late Lytic Genes
... levels of the wild-type, frameshift mutant, and revertant vi- ruses were comparable (Fig. 3A). Disruption of BDLF4 de- creased virus yield to approximately 10% to 20% compared with levels seen with the wild-type and ... See full document
5
The Essential Human Cytomegalovirus Gene UL52 Is Required for Cleavage-Packaging of the Viral Genome
... UL52 gene encoding pUL52 tagged with the HA epitope (dark gray box) at the C terminus was inserted at an ectopic position (the previous UL1-10 locus) into the UL52-deleted genome, resulting in the BAC pHG- ...UL52 ... See full document
14
Genetic and Molecular Analysis of spe-27, a Gene Required for Spermiogenesis in Caenorhabditis elegans Hermaphrodites
... Moreover, the observation that wild-type male sperm can respond to the hermaphrodite activator when inseminated without seminal fluid shows that male sperm can respond to[r] ... See full document
11
The foot-and-mouth disease virus leader proteinase gene is not required for viral replication.
... eukaryotic initiation factor 4F complex, which helps to shut off host protein synthesis. Cleavage of p220 appears to be important for picornavirus replication, since rhinoviruses and enteroviruses utilize a different ... See full document
7
The A2L intermediate gene product is required for in vitro transcription from a vaccinia virus late promoter.
... vitro late transcription on the A2L gene product as well as a factor purified from hydroxyurea-treated vaccinia virus-infected HeLa ...intermediate gene should not be expressed under these ... See full document
5
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