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[PDF] Top 20 Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing

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Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing

Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing

... coli 16S rRNA gene J01695 (Table ...Cycle sequencing was performed by standard protocols (27), including the use of a 45°C annealing temperature and the 16 sequencing primers listed in ... See full document

7

Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing

Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing

... distinct species (7, 15, 24, ...Campylobacter species could clearly be differentiated, since the minimum 16S rDNA sequence variation among the most related taxa exceeded the 3% threshold (Table ...of ... See full document

10

Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates

Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates

... the 16S rRNA gene sequence among the large number of Brucella strains tested is consistent with previous studies using a limited number of strains (5, 7) and could also be used to support proposals ... See full document

6

Streptococcus sinensis sp  nov , a Novel Species Isolated from a Patient with Infective Endocarditis

Streptococcus sinensis sp nov , a Novel Species Isolated from a Patient with Infective Endocarditis

... DNA sequencing, compar- ison of the gene sequences of bacterial species showed that the 16S rRNA gene is highly conserved within a species and among species of the ... See full document

6

Evaluation of 16S rRNA Sequencing and Reevaluation of a Short Biochemical Scheme for Identification of Clinically Significant Bacteroides Species

Evaluation of 16S rRNA Sequencing and Reevaluation of a Short Biochemical Scheme for Identification of Clinically Significant Bacteroides Species

... and 16S rRNA gene sequencing methods provided the same ...a 16S rRNA sequence match with a species other than the species assigned by phenotypic ...discrepant ... See full document

7

Unraveling the diversity of sedimentary sulfate-reducing prokaryotes (SRP) across Tibetan saline lakes using epicPCR

Unraveling the diversity of sedimentary sulfate-reducing prokaryotes (SRP) across Tibetan saline lakes using epicPCR

... tase gene (dsrAB) as a molecular marker [2, 17], a recent study using publicly available metagenomics data has suggested that the distribution of SRP phyla has been significantly underestimated in various ... See full document

12

Identification of Brachyspira hyodysenteriae and Other Pathogenic Brachyspira Species in Chickens from Laying Flocks with Diarrhea or Reduced Production or Both

Identification of Brachyspira hyodysenteriae and Other Pathogenic Brachyspira Species in Chickens from Laying Flocks with Diarrhea or Reduced Production or Both

... Brachyspira species were further examined using selective anaerobic culture, followed by phenotypic analysis, species-specific PCRs (for Brachyspira hyodysenteriae, ...partial 16S rRNA ... See full document

8

Identification of bacteria on the surface of clinically infected and non infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture

Identification of bacteria on the surface of clinically infected and non infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture

... different species were identified that are thought to be involved in pros- thetic hip joint infections; Staphylococcus species have previ- ously been described in this context [7,20,22], but the other ... See full document

11

The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays

The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays

... the 16S rRNA ...of specific primers using pub- lished Streptococcus sequences, filtered for quality and annotation-reliability by profile-based methods (Smart- Gene, Zug, ...published ... See full document

8

Real Time Quantitative Broad Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification

Real Time Quantitative Broad Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification

... Finally, sequencing of the amplicons generated using the primer pair Bak11W/Bak2 allowed species identification of the detected bacterial ...the 16S rRNA gene in the quantitative ... See full document

10

Identification of Pathogenic Nocardia Species by Reverse Line Blot Hybridization Targeting the 16S rRNA and 16S 23S rRNA Gene Spacer Regions

Identification of Pathogenic Nocardia Species by Reverse Line Blot Hybridization Targeting the 16S rRNA and 16S 23S rRNA Gene Spacer Regions

... with species-specific oligonucleotides in a reverse line blot (RLB) assay (20, 32, ...different gene targets within the same assay ...format species- and group-specific probes targeting ... See full document

9

16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “Clostridium neonatale”

16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “Clostridium neonatale”

... novel species of Clostridium, “Clostridium ...isolation, identification, or clinical signifi- cance of this ...new species, rendering its identification ...neonatale 16S rRNA ... See full document

8

Comparison of MALDI TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates

Comparison of MALDI TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates

... Accurate identification of ...However, species identification remains difficult using conventional ...the species level: a biochemical method, matrix-assisted laser desorption ionization mass ... See full document

6

Leptotrichia Bacteremia in Patients Receiving High Dose Chemotherapy

Leptotrichia Bacteremia in Patients Receiving High Dose Chemotherapy

... Leptotrichia species have occasionally been re- ported to cause invasive disease in HSCT patients and other im- munocompromised hosts (6, 14, ...phenotypic identification systems also have problems ... See full document

5

Identification of Lactobacilli and Bifidobacteria

Identification of Lactobacilli and Bifidobacteria

... that 16S ribosomal RNA (rRNA) sequences can be used as evolutionary chronometers ...the 16S rRNA molecule are conserved throughout all bacterial species and can be used to align ... See full document

13

Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections

Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections

... Two 16S rRNA gene PCR assays performed on the same sample detected ...a specific PCR confirmed these ...One 16S rRNA gene PCR assay detected Peptoniphilus ...bacterial ... See full document

11

Direct Identification of Mycobacteria in Primary Liquid Detection Media by Partial Sequencing of the 65 Kilodalton Heat Shock Protein Gene

Direct Identification of Mycobacteria in Primary Liquid Detection Media by Partial Sequencing of the 65 Kilodalton Heat Shock Protein Gene

... the identification of 20 isolates at a cost to our laboratory of ...direct identification (39 tests ⫻ $19) of ...complex identification was confirmed by the Accuprobe ...confirmed ... See full document

7

Study of Bacterial Diversity of Mangroves Rhizosphere

Study of Bacterial Diversity of Mangroves Rhizosphere

... for identification of ...with 16S rRNA sequencing when limited to a specific genus in case of marine microbes ...biochemical identification shows a harmony in results making the ... See full document

9

Clinical and Laboratory Diagnostic Characteristics and Cytotoxigenic Potential of Hafnia alvei and Hafnia paralvei Strains

Clinical and Laboratory Diagnostic Characteristics and Cytotoxigenic Potential of Hafnia alvei and Hafnia paralvei Strains

... Hafnia species may have occurred that they could be dis- tinguished on the basis of ampC gene ...acc-3 gene or the acc-3a ...acc-1 gene or variant acc- 1a, acc-1b, acc-1c, or acc-1d (P ⬍ ...to ... See full document

5

Catheter associated bacteremia by Mycobacterium senegalense in Korea

Catheter associated bacteremia by Mycobacterium senegalense in Korea

... the 16S rRNA gene, rpoB, and 16S-23S rRNA gene internal transcribe spacer (ITS) sequence analyses for bacterial ...of 16S rRNA gene, rpoB, and ITS were ... See full document

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