[PDF] Top 20 Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real Time PCR
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Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real Time PCR
... The PCR was carried out as described above. The PCR product was purified by adding 4 l of combined exonuclease I (10 U/ml) and shrimp alkaline phosphatase (2 U/l) (USB Corporation; distributor, Am- ... See full document
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Enhancing Gonococcal Antimicrobial Resistance Surveillance: a Real Time PCR Assay for Detection of Penicillinase Producing Neisseria gonorrhoeae by Use of Noncultured Clinical Samples
... noncultured clinical samples, providing 100% sensi- tivity and ...239 clinical specimens tested using both ...N. gonorrhoeae-negative samples and the samples tested in parallel with bacterial ... See full document
6
Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay
... for detection of pathogens in asymptomatic at-risk ...contrast, PCR and LCR assays utilizing urine, endocervical, or urethral samples have proven to be highly sensitive and specific and ... See full document
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Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens
... the detection of Chlamydia trachomatis and Neisseria ...the detection of infection in individuals with a low number of infectious units ...shown PCR (Roche Diagnostic Systems, Branchburg, ... See full document
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Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... the detection of DNA from serum, urine, and EDTA-anticoagulated blood, the most common materials from patients with invasive disease, was evaluated with spiking experiments, due to the absence of a corresponding ... See full document
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Characteristics of the m2000 Automated Sample Preparation and Multiplex Real Time PCR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
... and Neisseria gonorrhoeae are com- mon sexually transmitted ...invasive clinical samples, such as first-void urines (FVUs) and vaginal swabs, have been shown to be effective indicators of infection ... See full document
5
Detection of Coccidioides Species in Clinical Specimens by Real Time PCR
... Coccidioides PCR assay may be more sensitive than conventional laboratory methods, such as ...other PCR assays, it is sometimes difficult to interpret whether a positive PCR result indicates ... See full document
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Real Time PCR Assay for Detection of Quinolone Resistant Neisseria gonorrhoeae in Urine Samples
... urine specimens from N. gonorrhoeae-infected female patients who were tested as part of routine clinical ...urine specimens tested positive for N. gonorrhoeae at the San Francisco ... See full document
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sodC-Based Real-Time PCR for Detection of Neisseria meningitidis
... Culture detection of Nm is 100% specific and should be attempted if possible, but it is limited by low sensitivity [31,32] and 24- to 72-hour incubation ...Nm detection method in clinical and ... See full document
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Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of HBoV infection remain ...two sensitive and specific ... See full document
5
Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... of real-time-PCR, antigen detection, and tra- ditional-PCR assays on stool and liver abscess pus ...antigen detection test, traditional nested PCR, and ... See full document
5
Multiplex Real Time PCR Assay for Simultaneous Identification of Neisseria gonorrhoeae and Its Ciprofloxacin Susceptibility Status
... N. gonorrhoeae isolates (Table ...N. gonorrhoeae isolates were amplified and no non-N. gonorrhoeae isolates were amplified (Table ...N. gonorrhoeae isolates (a subset of panel 1) was analyzed by ... See full document
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Real Time PCR for Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar in Fecal Samples
... the specific identification of ...closed-tube, real-time PCR technology for the detection and differentiation of ...LightCycler PCR method employed here is fast, because it takes ... See full document
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Improved Safety for Molecular Diagnosis of Classical Rabies Viruses by Use of a TaqMan Real Time Reverse Transcription PCR “Double Check” Strategy
... ready-to-use, real-time reverse transcription-PCR (RT-PCR) assay was ...probes specific for rabies virus were selected from the consensus se- quence of the nucleoprotein gene of 203 ... See full document
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Real Time Reverse Transcription PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens
... the detection of immunoglobulin M-specific antibodies to mumps virus in acute-phase serum samples, the isolation of mumps virus in cell culture, or by detection of the RNA of the mumps virus by ... See full document
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Mismatch Amplification Mutation Assay Based Real Time PCR for Rapid Detection of Neisseria gonorrhoeae and Antimicrobial Resistance Determinants in Clinical Specimens
... identify clinical samples positive for some AMR mutations ...genital specimens (Table ...extragenital specimens, also typically associated with the inability to recover culture isolates, may also ... See full document
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Evaluation of Conventional and Real Time PCR Assays Using Two Targets for Confirmation of Results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae Test for Detection of Neisseria gonorrhoeae in Clinical Samples
... the detection of C. trachomatis and N. gonorrhoeae (4, 5, ...trachomatis/Neisseria gonorrhoeae (CA CT/NG) multiplex PCR test (Roche Diagnostics, Mannheim, Germany) provides a useful ... See full document
5
Comparison of an in house real time duplex PCR assay with commercial HOLOGIC® APTIMA assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urine and extra genital specimens
... N. gonorrhoeae infection due to antimicrobial resist- ance or reduced antimicrobial susceptibility are well docu- mented [23, ...N. gonorrhoeae infection of the oropharynx is more difficult to eradicate ... See full document
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Neisseria Species Identification Assay for the Confirmation of Neisseria gonorrhoeae Positive Results of the COBAS Amplicor PCR
... 408 specimens using the NsppID assay are shown in Table ...N. gonorrhoeae in only 102 (56%). Thirty-one specimens did not amplify detectable amplicons for any Neisseria ...with ... See full document
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Evaluation of the Abbott RealTime CT/NG Assay in Comparison to the Roche Cobas Amplicor CT/NG Assay
... for clinical testing in our hospital system and the Abbott RealTime CT/NG ...assay. Specimens were tested with the Roche system and either tested concurrently using the Abbott system or frozen at ⫺20°C and ... See full document
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