[PDF] Top 20 Structural studies of human immunodeficiency virus type 1 by cryo electron tomography

... Several observations revealed that a special capsid shape is heavily favored in vivo. Firstly, while purified CA protein can form sheets, rods, and various cones in different ratios depending on environmental conditions ... See full document

... Cellular structural biology by means of cryo-electron tomography (cryo-ET) offers unique insights into cellular processes at an unprecedented ...of electron microscopic imaging, ... See full document

... by cryo-ET. Nevertheless, even at lower resolution, valuable structural and functional information can be deduced, which often cannot be accomplished with biochemistry alone or by other structural ... See full document

... of Cryo-ET Cryo-ET has made great progress over the last decade, which is reflected by the increasing number of success- ful ...obtaining structural information for a mechanistic understanding of ... See full document

... used cryo-ET to study the bacteriophage infection cycle and a number of the specific events that take place have been visualized at unmatched resolution (Table ...of cryo-EM and cryo-ET, and the ... See full document

... on human health, many of the details of its particle assembly and structure remain poorly ...Previous structural analyses of HTLV-1 as- sembly have largely been limited to observing ... See full document

... influenza virus after 5 min at pH ...to studies in which fluorescence dequenching measurements were used to show that the fusion activity of X-31 virions was partly but not largely reduced by preincubation ... See full document

... line, and viral spread in the culture was monitored in the presence or absence of different concentrations of antibody. Viral replication curves were measured over an 8-day period (Fig. 4). D5-IgG1, which was suggested ... See full document

... Mature human immunodeficiency virus type 1 (HIV-1) particles contain a cone-shaped core structure consisting of the internal ribonucleoprotein complex encased in a proteinaceous ... See full document

... crystallographic studies have shown that the C terminus of the mature MA domain forms a 25- amino-acid-long ␣-helix that is distinct from the rest of the molecule ... See full document

... our structural data predict that some of these defective phe- notypes could have arisen simply because of a gross distortion of the overall structure of p17, rather than as a result of the substitution of a ... See full document

... and assembly of HIV-1. These constructs typically yield diffuse reticular staining throughout the cytoplasm along with addi- tional punctate signals of much brighter fluorescence intensity. These brighter signals ... See full document

... and electron microscopy to study the structure of human immunodeficiency virus type 1 (HIV-1) intracellular reverse transcription complexes ...microscopy. Electron ... See full document

... HIV-1 structural protein occurs during virion assembly. Nef FIG. 3. Electron microscopic analysis of HIV-1 ...with 1% uranyl ...transmission electron microscope operating at 120 ... See full document

... Rubella virus is the only member of the Rubivirus genus within the Togaviridae family and is the causative agent of the child- hood disease known as rubella or German ...of cryo-electron ... See full document

... scoring functions iteratively sort the subvolumes into classes based on relative similarity 18, 105, 109 . The recently developed autofocused 3D classification algorithm reduces human [r] ... See full document

... Members of the Paramyxoviridae such as measles, mumps, and parainfluenza viruses have pleomorphic, enveloped virions that contain negative-sense unsegmented RNA genomes. This is encapsidated by multiple copies of a viral ... See full document

... These mutants, wild type in structure but reduced in activity, identify residues in the wild-type protein that may directly contact other molecules during Tat function.. Human immunodefi[r] ... See full document

... PICs can be isolated from HIV-1-infected cells, and such particles are capable of carrying out integration reactions in vitro. We find that although the PICs are large, the cDNA must be condensed to fit into the ... See full document

... Abstract Cryo-electron tomography has been a valuable tool in the analysis of 3D structures of cilia at molecular and cellular ...2000s, cryo-electron tomography has provided us ... See full document