[PDF] Top 20 Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
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Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... the DNA substrate, all three W501 mutants (W501A, W501E, and W501R) showed significant unwinding activities ranging from 50 to 80% of that of the wild-type enzyme ...the RNA substrate, all three V432 ... See full document
12
Mutational analysis of the hepatitis C virus RNA helicase.
... the hepatitis C virus (HCV) NS3 protein has been shown to possess an RNA helicase activity, typical of members of the DEAD box family of RNA ...the ... See full document
10
RNA-Stimulated ATPase and RNA Helicase Activities and RNA Binding Domain of Hepatitis G Virus Nonstructural Protein 3
... G virus (HGV) nonstructural protein 3 (NS3) contains amino acid sequence motifs typical of ATPase and RNA helicase ...the RNA helicase activity of the ... See full document
7
Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... the NS3 helicase region of a hepatitis C virus (HCV) Con1 subgenomic replicon to ascertain the role of the helicase in viral ...the NS3 helicase ( ⌬ hel), and six ... See full document
8
Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
... bined helicase and nucleoside triphosphatase (NTPase), and an RNA-dependent RNA polymerase, are believed to be com- ponents of a complex responsible for viral RNA ...The C-terminal 450 ... See full document
10
Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... affect RNA helicase activity and the ...of RNA is an ATP-dependent process which requires ATP binding, ATP hydrolysis, and RNA ...ATPase activity is abol- ished will also impede ... See full document
9
The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase
... NS4A-stimulated RNA binding and unwinding by NS3 are essential for replicon ...of NS3 and strongly suggest that NS3 helicase activity and ATP-coupled RNA binding to ... See full document
8
The Arginine-1493 Residue in QRRGRTGR1493G Motif IV of the Hepatitis C Virus NS3 Helicase Domain Is Essential for NS3 Protein Methylation by the Protein Arginine Methyltransferase 1
... binding protein II, where Xaa is any amino acid, and in RG repeats in Sam68 (Src-associated protein during mitosis) were shown to be methylated by PRMT1, PRMT3, or yeast Rmt1 (5, ...Since ... See full document
14
Mutagenesis of the Dengue Virus Type 2 NS3 Protein within and outside Helicase Motifs: Effects on Enzyme Activity and Virus Replication
... putative RNA helicases of viral origin possess conserved amino acid sequence motifs enabling their classification into three distinct ...seven conserved motifs, while superfamily 3 has ... See full document
11
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... NS5B protein of the hepatitis C virus (HCV) is an RNA-dependent RNA polymerase (RdRp) ...be required for replication of the viral ...fusion protein was expressed ... See full document
13
Protease Inhibitors Block Multiple Functions of the NS3/4A Protease-Helicase during the Hepatitis C Virus Life Cycle
... protease activity of NS3 has been the focus of drug development efforts, NS3 is a bifunctional enzyme with sep- arate protease and helicase domains connected by a flexible ...The ... See full document
9
Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3′-Terminal Sequences of Viral Positive- and Negative-Strand RNA
... 3,000 amino acids, which is subsequently proteo- lytically processed by cellular signal peptidases and two HCV- encoded proteases to produce mature structural and functional proteins ...highly conserved se- ... See full document
14
Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
... NTPase activity. NS3FL was found to exhibit a substan- tially higher helicase activity than NS3H, but it did not affect the level of its NTPase activity ...the helicase without an ... See full document
11
Vaccinia virus gene A18R encodes an essential DNA helicase.
... vaccinia virus A18R protein is a DNA-dependent ATPase that contains the canonical sequence motifs associated with the DEXH group of DNA and RNA ...A18R protein function during ... See full document
9
A DNA-binding activity in BPV initiator protein E1 required for melting duplex ori DNA but not processive helicase activity initiated on partially single-stranded DNA
... E1 helicase structures (26,27) the domains of the collar adopt a symmetrical conforma- tion while the AAA+ domains are arranged asymme- ...domain DNA-binding segments assemble asym- metrically, tracking the ... See full document
9
Substitution of amino acid residue V1213 in the helicase domain of the genotype 3 hepatitis E virus reduces virus replication
... severe hepatitis in Japan, although the course of genotype 3 HEV infection is generally asymptomatic ...unique amino acid substitutions were ...the helicase or protease domain of HEV were ... See full document
8
Human Norovirus NS3 Has RNA Helicase and Chaperoning Activities
... NV NS3. (A) The NTPase activity of MBP-NS3 was measured as nanomoles of released inorganic phosphate in the presence of the indicated concentrations (0 to 10 mM) of ...standard RNA helix ... See full document
18
The RNA Helicase eIF4A Is Required for Sapovirus Translation
... PSaV RNA genome predicted by Mfold ...secondary RNA structures of Sapovirus genogroups I, II, IV, and V (Dresden, AY694184; Mc10, AY237420; Ehime 1107, DQ058829; and NK, AY646856) in schematic ... See full document
5
Vaccinia virus RNA helicase: nucleic acid specificity in duplex unwinding.
... The RNA-DNA hybrid structures that were so readily un- wound by NPH-II contained a 5 9 single-stranded tail on the bottom strand of the ...9 DNA tail was unwound just as well by NPH-II (data not ... See full document
5
The Amino Terminus of the Saccharomyces cerevisiae DNA Helicase Rrm3p Modulates Protein Function Altering Replication and Checkpoint Activity
... Rrm3p amino terminus nega- tively regulates protein ...of protein were loaded for each of the strains expressing rrm3 deletion al- ...full-length protein is marked with an ...of protein ... See full document
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