[PDF] Top 20 Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
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Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
... bined helicase and nucleoside triphosphatase (NTPase), and an RNA-dependent RNA polymerase, are believed to be com- ponents of a complex responsible for viral RNA ...The C-terminal 450 amino acids of the ... See full document
10
Polynucleotide modulation of the protease, nucleoside triphosphatase, and helicase activities of a hepatitis C virus NS3-NS4A complex isolated from transfected COS cells.
... the NS3-NS4A ATPase was enhanced to a similar extent (four- to fivefold) by saturating concentrations of poly(U), poly(A), poly(C), and poly(dC) whereas the ntNS3 ATPase was stimulated (five- to sixfold) by ... See full document
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Crystal Structure of the NS3 Protease-Helicase from Dengue Virus
... (NS3 helicase domain [NS3hel]) (44– 46), the NS5 methyltransferase (10), and the RNA-dependent RNA polymerase catalytic domain (27, 49, ...complete NS3 and NS5 polypeptide chains are still lacking. ... See full document
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Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... detailed structure of wild-type and mutant HCV helicase complexed with an ssRNA or ssDNA substrate by using molecular modeling ...W501A helicase mutant with respect to the wild-type helicase ... See full document
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Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase.
... the hepatitis C virus polyprotein is accomplished by a series of cotranslational and posttranslational cleavages mediated by host cell signalases and two virally encoded ...the NS3 proteinase ... See full document
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Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
... of NS3 helicase by HCV RdRp. Both NS3 helicase and NS5B RdRp are required for HCV RNA replication ...that NS3 colocal- ized with NS5B in the cell (24). Whether the NS3 ... See full document
11
Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a
... weeks based on the genotype of ...[14]. NS3 and NS5B are considered to be the main targets of this regime because they can be directly used for inhibitors screening due to their enzymatic properties ... See full document
12
Protease Inhibitors Block Multiple Functions of the NS3/4A Protease-Helicase during the Hepatitis C Virus Life Cycle
... and virus production prior to egress at a late stage during assembly or particle ...by NS3-de- pendent cleavage of the polyprotein, inhibition of RNA synthesis by a PI such as telaprevir could be a ... See full document
9
Upregulation of Protein Phosphatase 2Ac by Hepatitis C Virus Modulates NS3 Helicase Activity through Inhibition of Protein Arginine Methyltransferase 1
... PRMT1. NS3 helicase is one of the substrates of PRMT1 and becomes meth- ylated on critical arginine ...the helicase activity of NS3, leading to a decrease of viral (or replicon) ...the ... See full document
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The NS3 Helicase and NS5B-to-3′X Regions Are Important for Efficient Hepatitis C Virus Strain JFH-1 Replication in Huh7 Cells
... Significance of JFH-1 N5BX for replication. We demon- strated the importance of both the NS5B-coding region and the 3 ⬘ UTR in JFH-1 replication in the present study. There are several reports regarding CREs ... See full document
11
Mutational analysis of the hepatitis C virus RNA helicase.
... the hepatitis C virus (HCV) NS3 protein has been shown to possess an RNA helicase activity, typical of members of the DEAD box family of RNA ...the NS3 protein contains four ... See full document
10
Multiple Enzymatic Activities Associated with Recombinant NS3 Protein of Hepatitis C Virus
... HCV helicase to interact preferentially with the HCV genomic or antigenomic RNA has not been addressed in this ...ATP, NS3 protein binds ssRNA tightly with no particular se- quence specificity, analogous to ... See full document
12
Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase
... the helicase to assay dimerization and helicase ...in helicase activity of the three mu- tants is not due to their inability to bind the substrate, since all three mutants shifted the substrate to a ... See full document
10
Mutagenesis of the Dengue Virus Type 2 NS3 Protein within and outside Helicase Motifs: Effects on Enzyme Activity and Virus Replication
... The helicase of the flavivirus Dengue virus type 2 (DEN-2) is a member of su- perfamily 2, which includes the helicases of the pestivirus Bo- vine viral diarrhea virus (BVDV) and the hepacivirus ... See full document
11
NS3 from Hepatitis C Virus Strain JFH-1 Is an Unusually Robust Helicase That Is Primed To Bind and Unwind Viral RNA
... ABSTRACT Hepatitis C viruses (HCV) encode a helicase enzyme that is essential for viral replication and assembly (nonstructural protein 3 ...This helicase has be- come the focus of extensive ... See full document
16
Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease
... investigating NS3 protease inhibitors, such as substrate or product mimetics (27, 37), high-throughput screening of chem- ical compounds (19), and a selection of affinity ligands from a large pool of ... See full document
9
Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3′-Terminal Sequences of Viral Positive- and Negative-Strand RNA
... (or NS3 [data not shown]) was observed ...double-stranded structure was impor- tant for interaction with ...4), C-to-U substitutions reduced binding by only 32% (mutant IX; lanes 5 and ...When ... See full document
14
Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone
... infectious virus production and ...protease, helicase and polymerase) of HCV ...protease, helicase and poly- merase activity and thereby inhibit intracellular HCV replication and ...JFH-1 ... See full document
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Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... HCV NS3 helicase is strongly stimulated by the presence of RNA, which agrees with previous analysis of the ATPase activity of the wild-type helicase protein in the presence or absence of ... See full document
9
The helicase activity associated with hepatitis C virus nonstructural protein 3 (NS3).
... RNA helicase activity of hepatitis C virus (HCV) nonstructural protein 3 (NS3), a polypeptide encompassing amino acids 1175 to 1657, which cover only the putative helicase ... See full document
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