[PDF] Top 20 Studies on the purification and characterization of human factor VIII
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Studies on the purification and characterization of human factor VIII
... purified factor VIII has been found to be a macromolecular glycoprotein with a major subunit of 240,000, as shown by sodium dodecyl sulfate (SDS)-polyacrylamide gel ...of factor VIII gave ... See full document
12
Purification and characterization of elongation factor 2 (EF2) and cyclic AMP-independent protein kinases from soybean (Glycine max, L) cotyledons.
... sented in Fig. 1 show that EF2 elutes as a single peak. A second small peak of EF2 activity was observed just after the main peak, which possibly represents some low molecular weight ADPR accepting protein. It was ... See full document
155
Characterization and partial purification of a factor from uremic human serum that induces insulin resistance
... In this study insulin-stimulated glucose metabolism by normal rat adipocytes was used as a bioassay to identify the resistance activity, assess the effect of chemical modification on it,[r] ... See full document
7
Characterization and partial purification of an endothelial cell growth factor from human platelets
... the characterization and partial purification from human platelets of a novel growth factor which can stimulate human endothelial cells to synthesize DNA and ... See full document
6
Studies of the human factor VIII/von Willebrand factor protein III Qualitative defects in von Willebrand's disease
... Factor VIII/von Willebrand factor protein had markedly reduced von Willebrand factor activity in a ristocetin ...of Factor VIII/von Willebrand factor protein, antigen, and ... See full document
15
Understanding and Improving Platelet-Delivered Factor (F) VIII Hemostatic Efficacy in Hemophilia A Mice
... functional Factor (F) ...platelet human B-domainless factor VIII (phBF8) in the presence of circulating inhibitors in FVIIInull ...Willebrand Factor (vWF), efficiently, we tested the ... See full document
138
Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization
... the purification protocol of hG-CSF utilizes only one cation exchange column, which is an improvement upon previous protocols that utilized multiple chromatographic steps ...G-CSF purification protocol has ... See full document
12
Purification and partial characterization of a megakaryocyte colony stimulating factor from human plasma
... a factor that promotes megakaryocyte colony formation by normal human marrow ...colony-stimulating factor was purified from such a plasma ...step purification scheme which included ammonium ... See full document
10
Human factor VIII procoagulant protein Monoclonal antibodies define precursor product relationships and functional epitopes
... Proteolysis studies of purified VIII:C using thrombin and activated protein C have suggested that the 92,000- and 80,000 (or 72,000)-mol wt polypeptides comprise activated ... See full document
9
Demonstration and characterization of specific binding sites for factor VIII/von Willebrand factor on human platelets
... platelets. Binding is ristocetin-dependent and increases in proportion to the concentration of ristocetin from 0.2 to 1 mg/ml. Binding of 125I-FVIII/vWF to platelets can be competitively inhibited by unlabeled ... See full document
10
Studies on the purification of antihemophilic factor (factor VIII): II Separation of partially purified antihemophilic factor by gel filtration of plasma
... chylomicronpoor human plasma through columns of agarose. The final product contained, on the average, 67 units of antihemophilic activity per mg of protein, and was 3360-fold purified compared with the filtered ... See full document
7
Purification and further characterization of human mononuclear cell histamine releasing factor
... Since human interleukin 3 and granulocyte- macrophage colony-stimulating factor possess histamine-releasing capability, it is clear that multiple cytokines can share this ...from human mononuclear ... See full document
8
Studies on the purification of antihemophilic factor (factor VIII): I Precipitation Of Antihemophilic Factor By Concanavalin A
... antihemophilic factor from normal ...from human plasma with rapidity. The molecular weight of antihemophilic factor was estimated to be greater than 2 ...antihemophilic factor is a ... See full document
9
Expression, Characterization and Metallation Studies of Human Metallothionein Isoform 2a Using Electrospray Ionization Mass Spectrometry
... binding studies of MT2a showed that at physiological and higher pH, MT2a followed a non-cooperative metal binding pathway, which means that Zn binds first with terminal thiols, making bead-like structures with no ... See full document
69
Survival of 125iodine labeled Factor VIII in normals and patients with classic hemophilia Observations on the heterogeneity of human Factor VIII
... Radiolabeled human Factor VIII was used to study its survival in normals and patients with classic hemophilia, and to study the heterogeneity of Factor VIII; Purified Factor ... See full document
13
THE SURVIVAL OF FACTOR VIII (ANTIHEMOPHILIC GLOBULIN) AND FACTOR IX (PLASMA THROMBOPLASTIN COMPONENT) IN NORMAL HUMANS
... Half-lives and standard errors of estimate of the second components of the Factor VIII survival curves in five Figure 2 shows the survival curve for Factor normal human recipients VIII i[r] ... See full document
9
Purification and characterization of an extracellular alkaline serine protease from bacillus subtilis nr18
... proteases, bacterial proteases are the most significant, compared with plant, animal and fungal proteases, and world market with predominant use in detergents especially for alkaline bacterial proteases (Ward, ., 1992; ... See full document
6
Localization of the binding regions of a murine monoclonal anti factor VIII antibody and a human anti factor VIII alloantibody, both of which inhibit factor VIII procoagulant activity, to amino acid residues threonine351 serine365 of the factor VIII heavy chain
... anti-factor VIII (FVIII) inhibitory antibody (C5) to amino acid residues Thr351-Ser365 of the thrombin- generated 54-kD fragment of the heavy chain of ... See full document
7
Interaction of factor VIII antigen in hemophilic plasmas with human antibodies to factor VIII
... nonhemophiliac patient and in a hemophiliac patient gave similar results. The VIIIc antigen so identified was removed from hemophilic plasmas with immobilized rabbit antibody which reacted with normal VIIIc and von ... See full document
7
The role of BCL 2 protein family in the development of the peripheral nervous system
... extracellular domain bears four conserved cysteine repeats which have been demonstrated to be responsible for ligand recognition. The cysteine repeats are common to members of a family of cell surface receptors that ... See full document
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