[PDF] Top 20 Unexpected Structural Features of the Hepatitis C Virus Envelope Protein 2 Ectodomain

... The second potential CD81 binding region of E2 spans aa 522 to 551, and the third region is from aa 612 to 619. The E2c-Kong crystal structure provides clarity regarding which of these regions directly bind CD81. Most of ... See full document

... with hepatitis C virus (HCV), making HCV infection the leading cause of liver transplantation in developed ...HCV envelope glycoproteins (E1 and E2) are involved in receptor binding, ... See full document

... Hepatitis C virus has three structural genes named C, E1, and ...The C gene encodes the core (capsid) protein and the E1 and E2 genes encode the envelope ...E1 ... See full document

... simplex virus tk promoter (Fig. 2A). To determine which of the HCV structural proteins were affecting the grp78 and grp94 promoters, we used the same vector to drive the separate expression of each the ... See full document

... of hepatitis C virus (HCV) are intimately involved in orchestrating ...viral protein NS2 is suggested to play a central role in HCV particle ...NS2 protein-protein interactions ... See full document

... each virus, the sequence Ala-Thr-Gly is identical in HCV (aa 165 to 167) and GBV-B (aa 134 to 136), suggesting the same role in the capsid structure and/or ...the structural end of domain ... See full document

... 2000 Hepatitis C virus (HCV) is a major human pathogen causing chronic liver ...structure-function features of HCV-CD81 interaction by using recombinant E2 protein and a recombinant ... See full document

... of hepatitis C virus (HCV) remain incompletely ...infectious virus, we determined NS2 interaction partners among other HCV proteins during productive ...both structural and ... See full document

... In conclusion, we have shown that E2, which is presumably an ER-resident viral glycoprotein, is also found in large amounts as an unglycosylated protein in the cytosol. This new finding helps us to understand how ... See full document

... from protein sequences in the ab- sence of structural data, although it cannot distinguish a re- ceptor binding site from a site that interacts with another protein, whether viral or ...L ... See full document

... conserved features of the various structural re- gions were ...infectious virus pro- duction but indirectly affected RNA replication in the context of a monocistronic genome, most likely by ... See full document

... ABSTRACT Hepatitis C virus (HCV) envelope glycoprotein complex is composed of E1 and E2 ...receptor-binding protein as well as the major target of neutralizing antibodies, whereas the ... See full document

... HCV envelope glycoproteins play an essential role in virion morphogenesis as well as in HCV entry into liver ...which protein oligomerization often takes ...virion-associated envelope glycoproteins ... See full document

... The putative envelope protein of hepatitis C virus (HCV) was expressed in insect cells by using a baculovirus expression vector and in monkey COS cells under the control of exogenous pro[r] ... See full document

... by 2 log compared to HCVpp wild-type ...the envelope proteins in the viral particles, while the absence of infectivity of the W320A mutant indicates an effect of this mutation on virus ... See full document

... E1 protein, in vitro translation products of the HCV core and E1 polyprotein car- rying FLAG and HA tags in the N- and C-terminal regions of the E1 protein, respectively, were digested with trypsin, ... See full document

... Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that assemble as a noncovalent heterodimer which is mainly retained in the endoplasmic ...to structural changes in ... See full document

... HCV envelope proteins in ...gE2t-GST protein was efficiently recovered in coimmunopre- cipitates with anti-human lactoferrin antibody from the cell lysates as well as from the cell culture supernatants ... See full document

... E2 protein binds human CD81 ...certain protein molecules other than human CD81 and some glycosaminoglycans on the cell surface are also involved in the cell fusion induced by ... See full document

... France 2 ; and the Medical Research Council—University of Glasgow Centre for Virus Research, Glasgow, United Kingdom 3 Received 2 June 2010/Accepted 16 July 2010 Hepatitis C is caused ... See full document